Rice,Panicle,Apical abortion,QTL,Genetic analysis,"/> Genetic Analysis of Rice Mutant L-05261 with Panicle Apical Abortion Trait
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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 1935-1941.doi: 10.3724/SP.J.1006.2011.01935

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis of Rice Mutant L-05261 with Panicle Apical Abortion Trait

GAO Su-Wei,ZHANG Ling,MAO Bi-Gang,WANG Jiu-Lin,CHENG Zhi-Jun*,WAN Jian-Min   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-03-04 Revised:2011-06-25 Online:2011-11-12 Published:2011-09-06
  • Contact: 程治军, E-mail: chengzj5rs@yahoo.com.cn

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Abstract: In rice, panicle apical abortion (PAA) has a detrimental effect on the final yield. Using one PAA mutant L-05261, we found that the occurrence of PAA is related to the excess accumulation of hydrogen peroxides (H2O2). The hybrid plants of four cross-combinations from two less PAA varieties of 9311, PA64, and two non-PAA varieties of Balilla and IRAT129 crossed to L-05261 showed PAA phenotypes, on their F2 populations, the percentages of PAA showed a continuous distribution and a bias towards non-PAA parents. The ratio of PAA plants and non-PAA plants in the L-05261×IRAT129 F2 fitted to 63:1, while the ratio in the BC1F1consisted of 7:1, corresponding to a genetic model involving three or more partial dominant genes. One hundred and eighty-two individuals from F2 of L-05261×IRAT129 were employed for QTL analysis, and four QTLs designated as qPAA3, qPAA4, qPAA5, and qPAA8 respectively, were detected out.However, no significantinteraction was found among these QTLs. Totally, all four QTLs were able to explain 46.32% of observed phenotypic variation. The remaining phenotypic variation was likely caused by environment effect, which should be considered in the following study.

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[1]Wei X J, Xu J F, Guo H N, Jiang L, Chen S H, Yu C Y, Zhou Z L, Hu P S, Zhai H Q, Wan J M. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol, 2010, 153: 1747–1758
[2]Xue W Y, Xing Y Z, Weng X Y, Zhao Y, Tang W J, Wang L, Zhou H J, Yu S B, Xu C G, Li X H, Zhang Q F. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40: 761–767
[3]Ashikari M, Sakakibara H, Lin S Y, Yamamoto T, Takashi T, Nishimura A, Angeles E R, Qian Q, Kitano H, Matsuoka M. Cytokinin oxidase regulates rice grain production. Science, 2005, 309: 741–745
[4]Xing Y Z, Tang W J, Xue W Y, Xu C G, Zhang Q F. Fine mapping of a major quantitative trait loci, qSSP7, controlling the number of spikelets per panicle as a single Mendelian factor in rice. Theor Appl Genet, 2008, 116: 789–796
[5]Deshmukh R, Singh A, Jain N, Anand S, Gacche R, Singh A, Gaikwad K, Sharma T, Mohapatra T, Singh N. Identification of candidate genes for grain number in rice (Oryza sativa L.). Functional & Integrative Genomics, 2010, 10: 339–347
[6]Liu T M, Mao D H, Zhang S P, Xu C G, Xing Y Z. Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa). Theor Appl Genet, 2009, 118: 1509–1517
[7]Fan C C, Xing Y Z, Mao H L, Lu T T, Han B, Xu C G, Li X H, Zhang Q F. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet, 2006, 112: 1164–1171
[8]Wan X Y, Weng J F, Zhai H Q, Wang J K, Lei C L, Liu X L, Guo T, Jiang L, Su N, Wan J M. Quantitative Trait Loci (QTL) analysis for rice grain width and fine mapping of an identified QTL allele gw-5 in a recombination hotspot region on chromosome 5. Genetics, 2008, 179: 2239–2252
[9]Song X J, Huang W, Shi M, Zhu M Z, Lin H X. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet, 2007, 39: 623–630
[10]Shomura A, Izawa T, Ebana K, Ebitani T, Kanegae H, Konishi S, Yano M. Deletion in a gene associated with grain size increased yields during rice domestication. Nat Genet, 2008, 40: 1023–1028
[11]Bai X F, Luo L J, Yan W H, Kovi M R, Zhan W, Xing Y Z. Genetic dissection of rice grain shape using a recombinant inbred line population derived from two contrasting parents and fine mapping a pleiotropic quantitative trait locus qGL7. BMC Genet, 2010, 11: 16
[12]Wang E T, Wang J J, Zhu X D, Hao W, Wang L Y, Li Q, Zhang L X, He W, Lu B R, Lin H X, Ma H, Zhang G Q, He Z H. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nat Genet, 2008, 40: 1370–1374
[13]Li J M, Thomson M, McCouch S R. Fine mapping of a grain-weight quantitative trait locus in the Pericentromeric region of rice chromosome 3. Genetics, 2004, 168: 2187–2195
[14]Xie X B, Jin F X, Song M H, Suh J P, Hwang H G, Kim Y G, McCouch S R, Ahn S N. Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa × O. rufipogon cross. Theor Appl Genet, 2008, 116: 613–622
[15]Tabuchi M, Sugiyama K, Ishiyama K, Inoue E, Sato T, Takahashi H, Yamaya T. Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1:1. Plant J, 2005, 42: 641–651
[16]Zhou Y, Zhu J Y, Li Z Y, Yi C D, Liu J, Zhang H G, Tang S Z, Gu M H, Liang G H. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics, 2009, 183: 315–324
[17]Zhu K M, Tang D, Yan C J, Chi Z C, Yu H X, Chen J M, Liang J S, Gu M H, Cheng Z K. ERECT PANICAL2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics, 2010, 184: 343–350
[18]Ikeda K, Ito M, Nagasawa N, Kyozuka J, Nagato Y. Rice ABERRANT PANICLE ORGANIZATION 1, encoding an F-box protein, regulates meristem fate. Plant J, 2007, 51: 1030–1040
[19]Yamagishi J, Miyamoto N, hirotsu S, Laza R C, Nemoto K. QTLs for branching, floret formation, and pre-flowering floret abortion of rice panicle in a temperate japonica tropical japonica cross. Theor Appl Genet, 2004, 109: 1555–1561
[20]Li S B, Qian Q, Fu Z M, Zeng D L, Meng X B, Kyozuka J, Maekawa M, Zhu X D, Zhang J, Li J Y, Wang Y H. Short panicle1 encodes a putative PTR transporter and determines rice panicle size. Plant J, 2009, 58: 592–605
[21]Xu H-S(徐华山), Sun Y-J(孙永建), Zhou H-J(周红菊), Yu S-B(余四斌). Development and characterization of contiguous segment substitution lines with background of elite restorer line. Acta Agron Sin (作物学报), 2007, 33(6): 979–986 (in Chinese with English abstract)
[22]Tan C J, Sun Y J, Xu H S, Yu S B. Identification of quantitative trait locus and epistatic interaction for degenerated spikelets on the top of panicle in rice. Plant Breed, 2011, 130:177-184
[23]Isono P D, Varner J E. Hydrogen peroxide and lignification. Plant J, 1993, 4: 887–892
[24]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325
[25]Sanguinetti C J, Dias N E, Simpson A J G. Rapid silver staining and recover of PCR products separated on polyacrylamide gels. Biotechniques, 1994, 17: 915–919
[26]McCouch S R, Teytelman L, Xu Y B, Lobos K B, Clare K, Walton M, Fu B Y, Maghirang R, Li Z K, Xing Y Z, Zhang Q F, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9: 257–279
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