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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2123-2129.doi: 10.3724/SP.J.1006.2011.02123


Mining and Analyzing Genetic Diversity for Maize Rough Dwarf Disease Resistant Gerplasms and Its Application in Maize Breeding

XUE Lin1,ZHANG Dan2,XU Liang2,JIN Meng-Meng2,PENG Chang-Jun1,XU Chen-Wu2,*   

  1. 1 Jiangsu Yanjiang Institute of Agricultural Sciences, Rugao 226541, China; 2 Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China
  • Received:2011-03-14 Revised:2011-07-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 徐辰武, E-mail: qtls@yzu.edu.cn, Tel: 0514-87979358

Abstract: Maize rough dwarf disease generally caused by rice black-streaked dwarf virus (RBSDV)has been one of the main diseases in parts of maize production area in recent 10 years. Mining maize rough dwarf virus (MRDV) resistant germplasm and then breeding resistant variety is an economical and effective approach to control the disease. One hundred and eighty-four inbred lines including 111 common maize and 73 waxy maize were screened for reaction to MRDV under field conditions during 2009 and 2010 at Nantong, Jiangsu province, China. The genetic diversity of the tested materials was investigated based on 117 polymorphic SSR markers. Cluster analysis of 184 inbred lines was conducted based on genetic similarities from SSR data by using Powermarker 3.0 software with UPGMA method. The disease evaluation resulted in identification of two and eight inbred lines with high resistance and resistance to MRDV,respectively, among them two waxy inbred lines were identified. UPGMA analysis indicated that 184 inbred lines could be classified into nine sub-groups, which were generally consistent with their known pedigree information and breeder’s experiences. Two sub-groups that separately derived from 4S and US hybrid 78599 had better resistance to MRDV than other seven sub-groups and could be used as core germplasm in genetic improvement of maize for rough dwarf disease resistance in Jiangsu province.

Key words: Maize inbreeds, Maize rough dwarf disease, Resistance identification, Genetic diversity

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