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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 980-987.doi: 10.3724/SP.J.1006.2012.00980

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of Magnaporthe oryzae-Induced Promoter OsQ16p in Rice

WANG Guang,WU Zhi-Dan,ZHANG Lei,LIU Feng-Quan,SHAO Min*   

  1. College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China
  • Received:2011-12-22 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 邵敏, E-mail: minshao@njau.edu.cn

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Abstract: The qRT-PCR analysis showed that, in Nipponbare (Oryza sativa L. ssp japonica), the expressionof OsQ16 gene was induced by Magnaporthe grisea. The 1 299 bp-fragment of 5'-end of OsQ16 gene, named as OsQ16p,was amplified by PCR from Nipponbare. The plasmids pBIQ16p was constructed by replacing the CaMV35S promoter of pBI121 with the OsQ16p, and transformed into Nipponbare through Agrobacterium-mediated transformation. The analysis of GUS activity and qRT-PCR showed that gus gene could express in transgenic plants and callui. The expression of gus gene in transgenic plants was obviously enhanced by M. grisea. After treatment with the resistance-related signaling molecules SA and MeJA, the GUS activities in transgenic plants were increased by 3.1- and 3.5-fold, respectively. It suggested that M. grisea, SA and MeJA were inductive factors of OsQ16p promoter.

Key words: Transgenic rice, Inducible promoter, OsQ16p, GUS, Magnaporthe grisea

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