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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1254-1258.doi: 10.3724/SP.J.1006.2017.01254

• RESEARCH NOTES • Previous Articles    

Application of Pisum sativum Terminator rbc-T in Wheat Transformation

WANG Xiao-Ting1,2,HUANG Suo2,XU Zhao-Shi2,LI Lian-Cheng2,MA You-Zhi2,CHEN-Ming2,*,MIN Dong-Hong1,*   

  1. 1College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-01-20 Revised:2017-04-20 Online:2017-08-12 Published:2017-04-27
  • Contact: Min Donghong, E-mail: mdh2493@126.com, Tel: 13609123593; Chen Ming, E-mail: chenming02@caas.cn, Tel: 010-82108750 E-mail:daisy19920829@foxmail.com
  • Supported by:

    This study was supported by the National Major Project for Developing New GM Crops (2014ZX08002-003B, 2016ZX08002002, 2016ZX08002005).

Abstract:

The fungal- or bacterial-origin vector elements are commonly used in transgenic crops, which raise great concerns about the safety of GM foods. In the study, we isolated the pea (Pisum sativum) terminator, rbc-T, and constructed a transformation vector by replacing the Agrobacterium terminator nos-T with rbc-T. The minimum transformation fragment consisting of Ubiquitin promoter, GUS reporter gene and terminator rbc-T was transformed into common wheat by particle bombardment method. Meanwhile a minimum transformation fragment with ?the Agrobacterium terminator nos-T was used as the control. The T2 transgenic lines were regenerated after PCR validation of the transformation. In the transgenic lines carrying both kinds of terminators, GUS gene expression was detected by GUS histochemical staining and enzyme activity analysis, indicating that the pea terminator rbc-T is applicable as an alternative of the Agrobacterium terminator nos-T in transgenic wheat research.

Key words: Nopaline synthase terminator (nos-T), Pea terminator (rbc-T), Transgenic wheat, GUS reporter gene

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