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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 22-28.doi: 10.3724/SP.J.1006.2014.00022

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of AcAMP-sn Transgenic Wheat with Enhanced Resistance to Wheat Take-all

YANG Kun,LIU Xin,DU Li-Pu,YE Xing-Guo,ZHANG Zeng-Yan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-06-17 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 张增艳, E-mail: zhangzengyan@caas.cn, Tel: 010-82108781

Abstract:

Antimicrobial peptides, small molecular weight proteins with broad spectrum antimicrobial activity, play important roles in plant defense responses. The open-reading-frame sequence of AcAMP-sn was synthesized and used to construct the gene transformation vector pAHC25::AcAMP-sn, in which AcAMP-sn gene was driven by maize ubiquitin promoter and should be highly expressed in monocot plants. The vector pAHC25::AcAMP-sn DNA was introduced into Yangmai 18 via particle bombardment. AcAMP-sn transgenic wheat plants were subjected to PCR, semi-RT-PCR, Q-RT-PCR analyses, and disease response assessments. PCR analyses revealed that theintroducedgene AcAMP-sn could be stably inherited in five transgenic wheat lines from T0 to T4 generations. Semi-RT-PCR and Q-RT-PCR analyses showed that the AcAMP-sn gene was highly expressed in transgenic wheat lines compared with untransformed Yangmai 18. Based on disease response assessments for T4 generations, the significantly enhanced-resistance to take-all accompanied with decreased fungal abundance, in the five independent AcAMP-sn transgenic lines. Over-expression of AcAMP-sn gene in transgenic wheat plants confers increased resistance to wheat take-all.

Key words: Antimicrobial peptide from onion, Transgenic wheat, Wheat take-all, Resistance

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