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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 29-36.doi: 10.3724/SP.J.1006.2014.00029

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning a Novel Gene TaNRX of Trx Superfamily and Developing Its Molecular Markers Related to Drought Resistance in Common Wheat

ZHANG Fan,JIANG Lei,JU Li-Ping,JIN Xiu-Feng,WANG Xuan,ZHANG Xiao-Ke*,WANG Hong-Li,FU Xiao-Jie   

  1. College of Agronomy, Northwest A&F University / Yangling Subcenter of National Wheat Improvement Center, Yangling 712100, China
  • Received:2013-05-06 Revised:2013-08-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 张晓科, E-mail: zhangxiaoke66@126.com

Abstract:

Molecular markers associated with genes for drought resistance play an important role in wheat breeding aiming at improvement of drought resistance. In this study, we obtained the full-length cDNA of a novel gene of thioredoxin (Trx) superfamily, TaNRX (GenBank accession number KC890769), from common wheat (Triticum aestivumresidues with a putative molecular mass of 63.79 kD. The TaNRX is composed of three Trx-like modules arranged as direct repeats of the classic Trx domain. The first and third modules contain the amino acid sequence Cys-X1-X2-Cys, with the potential for Trx oxidoreductase activity. Gene TaNRXcomplementary dominant markers were developed. In 150 wheat varieties (lines), two genotypes on TaNRX locus, TaNRX-a and TaNRX-b, were identified to be associated with drought resistance. As revealed by the four molecular markers, the average RGer in TaNRX-a genotype was significantly higher than that in TaNRX-b genotype (P < 0.01). This result suggests that the molecular markers developed in this study are effective to be used in selection of wheat varieties with drought resistance. has four exons and three introns, and is mapped on chromosome 5BS of wheat. The variations of TaNRX sequences were mainly concentrated in the first intron according to genomic sequence variances analysis between two types of varieties with different relative germination rates (RGer). Based on the variations within the first intron of TaNRX, four L.) using the protocol of gene homology cloning, electronic cloning, RACE, and bioinformatics analysis. The open reading frame (ORF) of TaNRX is 1734 bp in length, and the 5′ and 3′ UTRs are 99 and 182 bp, respectively. The ORF encodes a protein of 577 amino acid

Key words: Common wheat, Drought resistance, TaNRX, Molecular markers

[1]Zheng W J, Xu Z S, Chen M, Li L C, Chai S C, Ma Y Z. Isolation and characterization of receptor-like protein kinase WELP1 in wheat. Afr J Microbiol Res, 2012, 6: 2410–2418



[2]杨召恩, 杨作仁, 刘坤, 刘传亮, 张朝军, 李付广. 一个亚洲棉MYB家族新基因的克隆及特征分析. 中国农业科学, 2013, 46: 195–204



Yang Z E, Yang Z R, Liu K, Liu C L, Zhang C J, Li F G. Cloning and characterization of a novel gene of MYB family from Gossypium arboreum L. Sci Agric Sin, 2013, 46: 195–204 (in Chinese with English abstract)



[3]Shinozaki K, Yamaguchi-Shinozaki K. Gene networks involved in drought stress response and tolerance. J Exp Bot, 2007, 58: 221–227



[4]Kosová K, Vítámvás P, Prášil I T, Renaut J. Plant proteome changes under abiotic stress: contribution of proteomics studies to understanding plant stress response. J Proteomics, 2011, 74: 1301–1322



[5]Caruso G, Cavaliere C, Foglia P, Gubbiotti R, Samperi R, Laganà A. Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry. Plant Sci, 2009, 177: 570–576



[6]Peng Z, Wang M, Li F, Lv H, Li C, Xia G. A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. Mol Cell Proteomics, 2009, 12: 2676–2686



[7]Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem, 2010, 48: 909–930



[8]Arnér E S J, Holmgren A. Physiological functions of thioredoxin and thioredoxin reductase. Eur J Biochem, 2001, 267: 6102–6109



[9]Wong J H, Balmer Y, Cai N, Tanaka C K, Vensel W H, Hurkman W J, Buchanan B B. Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics. FEBS Lett, 2003, 547: 151–156



[10]Meyer Y, Vignols F, Reichheld J P. Classification of plant thioredoxins by sequence similarity and intron position. Methods Enzymol, 2002, 347: 394–402



[11]夏德习, 管清杰, 金淑梅, 李宇佳, 梁涵, 张欣欣, Shunskau N, Tetsuo T, 柳参奎. 拟南芥硫氧还蛋白M1型基因(AtTRX m1)与环境逆境之间的关系. 分子植物育种, 2007, 5: 21–26



Xia D X, Guan Q J, Jin S M, Li Y J, Liang H, Zhang X X, Shunskau N, Tetsuo T, Liu S K. The relationship of Arabidopsis thaliana thioredoxin M-type 1 (AtTRX m1) gene with environmental stress. Mol Plant Breed, 2007, 5: 21–26 (in Chinese with English abstract)



[12]Broin M, Rey P. Potato plants lacking the CDSP32 plastidic thioredoxin exhibit overoxidation of the BAS1 2-cysteine peroxiredoxin and increased lipid peroxidation in thylakoids under photooxidative stress. Plant Physiol, 2003, 132: 1335–1343



[13]Laughner B J, Sehnke P C, Ferl R J. A novel nuclear member of the thioredoxin superfamily. Plant physiol, 1998, 118: 987–996



[14]Deshmukh V, Kurtkoti R. Secondary Structure Prediction and Phylogenetic Analysis of Salt Tolerant Proteins. Global J Mol Sci, 2010, 5: 30–36



[15]Morya V K., Yadav S, Kim E K, Yadav D. In silico characterization of alkaline proteases from different species of Aspergillus. Appl Biochem Biotechnol, 2012, 166: 243–257



[16]Akash M S H, Rehman K, Gillani Z, Sun H, Chen S. Cross-species amino acids sequence comparison and computational docking of human IL-1Ra and rat IL-1Ra on rat receptor. J Proteomics Bioinform, 2013, 6: 38–42



[17]Chen Z, Ao J, Yang W, Jiao L, Zheng T, Chen X. Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment. Appl Microbiol Biotechnol, 2013, DOI 10.1007/s00253-013-4800-6



[18]Zhang A, Qiu L, Huang L, Yu X, Lu G, Cao J. Isolation and characterization of an anther-specific polygalacturonase gene, BcMF16, in Brassica campestris ssp. chinensis. Plant Mol Biol Rep, 2012, 30: 330–338



[19]Steinway S, Dannenfelser R, Laucius C, Hayes J, Nayak, S. JCoDA: a tool for detecting evolutionary selection. BMC Bioinf, 2010, 11: 284



[20]Ma W, Zhang W, Gale K R. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica, 2003, 134: 51–60



[21]Guruprasad K, Reddy B V, Pandit M W. Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence. Protein Eng, 1990, 4: 155–161



[22]Kurooka H, Kato K, Minoguchi S, Takahashi Y, Ikeda J E, Habu S, Osawa N, Buchberg A M, Moriwaki K, Shisa H, Honjo T. Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin. Genomics, 1997, 39: 331–339



[23]Funato Y, Miki H. Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation. Antioxidants & Redox Signaling, 2007, 9: 1035–1058



[24]王磊, 陈景堂, 张祖新. 主要禾谷类作物比较基因组学研究策略与进展. 遗传, 2007, 29: 1055–1060



Wang L, Chen J T, Zhang Z X. Strategies and progresses on cereal comparative genomics. Hereditas, 2007, 29: 1055–1060 (in Chinese with English abstract)



[25]周江鸿, 赵素珍, 漆小泉. 短柄草与麦类作物的比较基因组学研究进展. 植物生理学报, 2011, 47: 421–426



ZhouJ H, Zhao S Z, Qi X Q. Recent progresses in comparative genomics of Brachypodium and Triticeae crops. Plant Physiol J, 2011, 47: 421-426 (in Chinese with English abstract)



[26]He X Y, Zhang Y L, He Z H, Wu Y P, Xiao Y G, Ma C X, Xia X C. Characterization of phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker. Theor Appl Genet, 2008, 116: 213–221



[27]Fu D, Sz?cs P, Yan L, Helguera M, Skinner J S, von Zitzewitz J, Hayes M, Dubcovsky J. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Mol Genet Genomics, 2005, 273: 54–65

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