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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1904-1909.doi: 10.3724/SP.J.1006.2011.01904

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Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice

FANG Xiao-Liang1,2,3,LIU Wei1,2,3,*,AN Jing1,WANG Qing-Guo1,2   

  1. 1 High-Tech Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
  • Received:2011-03-07 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572

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Abstract: A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG, anda 1.4 kb fragement named OsESP1was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating theOsESP1 is an embryo-specific promoter.

Key words: Rice, Embryo-specific promoter, Expression patern analysis, GUS reporter gene

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