Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1904-1909.doi: 10.3724/SP.J.1006.2011.01904

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice

FANG Xiao-Liang1,2,3,LIU Wei1,2,3,*,AN Jing1,WANG Qing-Guo1,2   

  1. 1 High-Tech Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
  • Received:2011-03-07 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572

PDF

1491

Cited

1

Abstract: A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG, anda 1.4 kb fragement named OsESP1was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating theOsESP1 is an embryo-specific promoter.

Key words: Rice, Embryo-specific promoter, Expression patern analysis, GUS reporter gene

[1]Stone J M, Walker J C. Plant protein kinase families and signal transduction. Plant Physiol, 1995, 108: 451?457
[2]Li J, Chory J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell, 1997, 90: 929?938
[3]Stein J C, Howlett B, Boyes D C. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of B rassicaoleracea. Prot Natl Acad Sci USA, 1991, 88: 8816?8820
[4]Song W Y, Wang G L, Chen L L. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270: 1804?1806
[5]Stone S L, Kwong L W, Yee K M, Pelletier J, Lepiniec L, Fischer R L, Coldberg R B, Harada J J. LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development. Prot Natl Acad Sci USA, 2001, 98: 11806?11811
[6]Cai M, Wei J, Li X, Xu C, Wang S. A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific biotechnology gene expression in transgenic plants. Plant J, 2007, 5: 664?674
[7]Zhang X-Y(张宪银), Xue Q-Z(薛庆中). Cloning of a rice endosperm-specific promoter Gt l and its functional verifiction. Acta Agron Sin (作物学报), 2002, 28(1): 110?114 (in Chinese with English abstract)
[8]Qu L Q, Takaiwa F. Evaluation of tissue specificity and expression strength of rice seed component gene promoters in transgenic rice. Plant Biotech J, 2004, 2: 113?125
[9]Danny W, Timothy C. PvALF and FUS3 activate expression from the phaseolin promoter by different mechanisms. Plant Mol Biol, 2008, 66: 233?244
[10]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321?4325
[11]Hofgen R, Willmitzer L. Storage of competent cell for agrobacterium transformation. Nucl Acids Res, 1988, 16: 9877
[12]Liu Q-Q(刘巧泉), Gu M-H(顾铭洪). A highly efficient transformation system mediated by agrobacterium tumefaciens in rice (Oryza sativa L.). J Plant Physiol Mol Biol (植物生理学报), 1998, 24: 259?271 (in Chinese with English abstract)
[13]Jefferson R A. Assaying chimeric genes in plants: the GLJS gene fusion system. Plant Mol Biol Rep, 1987, 5: 387?405
[14]Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucl Acids Res, 27: 297?300
[15]Yamaguchi-Shinozaki K, Shinozaki K. Arabidopsis DNA encoding two desiccation-responsive rd29 genes. Plant Physiol, 1993, 101: 1119?1120
[16]Reyes J C, Muro-Pastor M I, Florencio F J. The GATA family of transcription factors in Arabidopsis and rice. Plant Physiol, 2004, 134: 1718?1732
[17]Nishiuchi T, Shinshi H, Suzuki K. Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves: possible involvement of NtWRKYs and autorepression. J Biol Chem, 2004, 279: 55355?55361
[18]Ellerstrom M, Stalberg K, Ezcurra I, Rask L. Functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcription. Plant Mol Biol, 1996, 32: 1019?1027
[19]Kim S Y, Chung H J. Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo- specification elements in the Dc3 promoter using a modified yeast one-hybrid system. Plant J, 1997, 11: 1237?1251
[20]Thomas M S, Flavell R B. Identification of an enhancer element for the endosperm-specific expression of high molecular weight glutenin. Plant Cell, 1990, 2: 1171?1180
[21]Rubio-Somoza I, Martinez M, Abraham Z, Diaz I, Carbonero P. Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds. Plant J, 2006, 47: 269?281
[22]Bate N, Twell D. Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol, 1998, 37: 859?869
[23]Shirsat A, Wilford N, Croy R, Boulter D. Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco. Mol Gen Genet, 1989, 215: 326?331
[24]Yoshida K, Shinmyo A. Transgene expression systems in plant, a natural bioreactor. J Biosci Bioeng, 2000, 90: 353?362
[25]Rossak M, Smith M, Kunst L. Expression of the FAE1 gene and FAE1 promoter activity in developing seeds of Arabidopsis thaliana. Plant Mol Biol, 2001, 46: 717?725
[26]Kuwano M, Mimura T, Talcaiwa F. Generation of stable ‘low phytic acid’ transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene (RINOl) using the 18-kDa oleosin promoter. Plant Biotech J, 2009, 7: 96?105
[27]Wu C Y, Adachi T, Hatano T, Washida H, Suzuki A, Takaiwa F. Promoters of rice seed storage protein genes direct endosperm-specific gene expression in transgenic rice. Plant Cell Physiol, 1998, 39: 885?889
[28]Hwang Y S, Yang D, Mccuar C. Analysis of the rice specific globulin promoter in transfomed rice cells. Plant Cell, 2002, 20: 842?847
[29]Jose-Estanyol M, Perez P. Expression of the promoter of HyPRP, an embryo-specific gene from Zea mays in maize and tobacco transgenic plants. Gene, 2005, 356: 146?152
[30]Hwang Y S, Nichol S, Nandi S, Jernstedt J. Aleurone- and embryo-specific expression of the β-glucuronidase gene controlled by the barley Chi26 and Ltp1 promoters in transgenic rice. Plant Cell Rep, 2001, 20: 647?654
[31]Doshi K M, Eudes F, Laroche A, Gaudet D. Transient embryo-specific expression of anthocyanin in wheat. Biomed Life Sci, 2006, 42: 432?438
[32]Kim J H, Jung I J, Kim D Y, Fanata W I, Son B H, Yoo J Y, Harmoko R, Ko K S, Moon J C, Jang H H, Kim W Y, Kim J Y, Lim C O, Lee S Y, Lee K O. Proteomic identification of an embryo-specific 1Cys-Prx promoter and analysis of its activity in transgenic rice. Biochem Biophys Res Commun, 2011, 408: 78?83
[33]Gu Y-H(古英红), Tang H-R(汤浩茹), Zhang Y-Z(张义正). Molecular cloning, sequence analysis of polygalacturonase inhibiting protein gene from Prunus persica and its expression in E. coli. Sci Agric Sin (中国农业科学), 2008, 41(10): 3191?3199 (in Chinese with English abstract)
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[15] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd