Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1904-1909.doi: 10.3724/SP.J.1006.2011.01904

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice

FANG Xiao-Liang1,2,3,LIU Wei1,2,3,*,AN Jing1,WANG Qing-Guo1,2   

  1. 1 High-Tech Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
  • Received:2011-03-07 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572

PDF

1829

Cited

1

Abstract: A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG, anda 1.4 kb fragement named OsESP1was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating theOsESP1 is an embryo-specific promoter.

Key words: Rice, Embryo-specific promoter, Expression patern analysis, GUS reporter gene

[1]Stone J M, Walker J C. Plant protein kinase families and signal transduction. Plant Physiol, 1995, 108: 451?457
[2]Li J, Chory J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell, 1997, 90: 929?938
[3]Stein J C, Howlett B, Boyes D C. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of B rassicaoleracea. Prot Natl Acad Sci USA, 1991, 88: 8816?8820
[4]Song W Y, Wang G L, Chen L L. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270: 1804?1806
[5]Stone S L, Kwong L W, Yee K M, Pelletier J, Lepiniec L, Fischer R L, Coldberg R B, Harada J J. LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development. Prot Natl Acad Sci USA, 2001, 98: 11806?11811
[6]Cai M, Wei J, Li X, Xu C, Wang S. A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific biotechnology gene expression in transgenic plants. Plant J, 2007, 5: 664?674
[7]Zhang X-Y(张宪银), Xue Q-Z(薛庆中). Cloning of a rice endosperm-specific promoter Gt l and its functional verifiction. Acta Agron Sin (作物学报), 2002, 28(1): 110?114 (in Chinese with English abstract)
[8]Qu L Q, Takaiwa F. Evaluation of tissue specificity and expression strength of rice seed component gene promoters in transgenic rice. Plant Biotech J, 2004, 2: 113?125
[9]Danny W, Timothy C. PvALF and FUS3 activate expression from the phaseolin promoter by different mechanisms. Plant Mol Biol, 2008, 66: 233?244
[10]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321?4325
[11]Hofgen R, Willmitzer L. Storage of competent cell for agrobacterium transformation. Nucl Acids Res, 1988, 16: 9877
[12]Liu Q-Q(刘巧泉), Gu M-H(顾铭洪). A highly efficient transformation system mediated by agrobacterium tumefaciens in rice (Oryza sativa L.). J Plant Physiol Mol Biol (植物生理学报), 1998, 24: 259?271 (in Chinese with English abstract)
[13]Jefferson R A. Assaying chimeric genes in plants: the GLJS gene fusion system. Plant Mol Biol Rep, 1987, 5: 387?405
[14]Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucl Acids Res, 27: 297?300
[15]Yamaguchi-Shinozaki K, Shinozaki K. Arabidopsis DNA encoding two desiccation-responsive rd29 genes. Plant Physiol, 1993, 101: 1119?1120
[16]Reyes J C, Muro-Pastor M I, Florencio F J. The GATA family of transcription factors in Arabidopsis and rice. Plant Physiol, 2004, 134: 1718?1732
[17]Nishiuchi T, Shinshi H, Suzuki K. Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves: possible involvement of NtWRKYs and autorepression. J Biol Chem, 2004, 279: 55355?55361
[18]Ellerstrom M, Stalberg K, Ezcurra I, Rask L. Functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcription. Plant Mol Biol, 1996, 32: 1019?1027
[19]Kim S Y, Chung H J. Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo- specification elements in the Dc3 promoter using a modified yeast one-hybrid system. Plant J, 1997, 11: 1237?1251
[20]Thomas M S, Flavell R B. Identification of an enhancer element for the endosperm-specific expression of high molecular weight glutenin. Plant Cell, 1990, 2: 1171?1180
[21]Rubio-Somoza I, Martinez M, Abraham Z, Diaz I, Carbonero P. Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds. Plant J, 2006, 47: 269?281
[22]Bate N, Twell D. Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol Biol, 1998, 37: 859?869
[23]Shirsat A, Wilford N, Croy R, Boulter D. Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco. Mol Gen Genet, 1989, 215: 326?331
[24]Yoshida K, Shinmyo A. Transgene expression systems in plant, a natural bioreactor. J Biosci Bioeng, 2000, 90: 353?362
[25]Rossak M, Smith M, Kunst L. Expression of the FAE1 gene and FAE1 promoter activity in developing seeds of Arabidopsis thaliana. Plant Mol Biol, 2001, 46: 717?725
[26]Kuwano M, Mimura T, Talcaiwa F. Generation of stable ‘low phytic acid’ transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene (RINOl) using the 18-kDa oleosin promoter. Plant Biotech J, 2009, 7: 96?105
[27]Wu C Y, Adachi T, Hatano T, Washida H, Suzuki A, Takaiwa F. Promoters of rice seed storage protein genes direct endosperm-specific gene expression in transgenic rice. Plant Cell Physiol, 1998, 39: 885?889
[28]Hwang Y S, Yang D, Mccuar C. Analysis of the rice specific globulin promoter in transfomed rice cells. Plant Cell, 2002, 20: 842?847
[29]Jose-Estanyol M, Perez P. Expression of the promoter of HyPRP, an embryo-specific gene from Zea mays in maize and tobacco transgenic plants. Gene, 2005, 356: 146?152
[30]Hwang Y S, Nichol S, Nandi S, Jernstedt J. Aleurone- and embryo-specific expression of the β-glucuronidase gene controlled by the barley Chi26 and Ltp1 promoters in transgenic rice. Plant Cell Rep, 2001, 20: 647?654
[31]Doshi K M, Eudes F, Laroche A, Gaudet D. Transient embryo-specific expression of anthocyanin in wheat. Biomed Life Sci, 2006, 42: 432?438
[32]Kim J H, Jung I J, Kim D Y, Fanata W I, Son B H, Yoo J Y, Harmoko R, Ko K S, Moon J C, Jang H H, Kim W Y, Kim J Y, Lim C O, Lee S Y, Lee K O. Proteomic identification of an embryo-specific 1Cys-Prx promoter and analysis of its activity in transgenic rice. Biochem Biophys Res Commun, 2011, 408: 78?83
[33]Gu Y-H(古英红), Tang H-R(汤浩茹), Zhang Y-Z(张义正). Molecular cloning, sequence analysis of polygalacturonase inhibiting protein gene from Prunus persica and its expression in E. coli. Sci Agric Sin (中国农业科学), 2008, 41(10): 3191?3199 (in Chinese with English abstract)
[1] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[2] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[3] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[4] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[5] Shi Shao-Jie, Liu Kai, Chen Zi-Yi, Wang Hui-Ying, Li San-He, Zhou Lei, You Ai-Qing. Cloning and functional analysis of the dwarf and multi-tiller gene DMT1 in rice [J]. Acta Agronomica Sinica, 2026, 52(4): 1022-1034.
[6] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[7] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[8] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[9] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[10] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[11] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[12] GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng. Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive [J]. Acta Agronomica Sinica, 2025, 51(9): 2433-2453.
[13] CHEN Hui-Ying, HE Jia-Xin, ZHU Bin, HUANG Shi-Xuan, ZHOU Xing-You, WU Jun-Quan, YANG Mei-Yan. Whole genome analysis and biological characterization of phage vB_XaS_ HDB2 infected with Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2025, 51(8): 2087-2099.
[14] YANG Hai-Yang, WU Lin-Xuan, LI Bo-Wen, SHI Han-Feng, YUAN Xi-Long, LIU Jin-Zhao, CAI Hai-Rong, CHEN Shi-Yi, GUO Tao, WANG Hui. OsWRI3, identified based on QTL mapping, regulates seed shattering in rice [J]. Acta Agronomica Sinica, 2025, 51(7): 1712-1724.
[15] WANG Fen, WU Dong-Li, ZHANG Quan-Jun. Response of phenological phase stages of single-cropping rice to climate change in Hubei province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1934-1948.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd