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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 789-794.doi: 10.3724/SP.J.1006.2017.00789


Designing, Construction and Functional Characterization of Tissue-specific Synthetic Promoter in Rice

WANG Rui1,2,ZHU Meng-Lin1,GAO Fang-Yuan2,REN Juan-Sheng2,LU Xian-Jun2,REN Guang-Jun2,LIN Yong-Jun1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
  • Received:2016-11-05 Revised:2017-03-01 Online:2017-06-12 Published:2017-03-24
  • Contact: Lin Yongjun,E-mail: yongjunlin@mail.hzau.edu.cn E-mail:wangray1987@163.com
  • Supported by:

    This study was supported by the National Major Project for Developing New GM Crops (2014ZX08001001) and the National High Technology Research and Development Program of China (863 Program)a.


As an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. Rice is one of the most important food crops in the world and a model plant for functional genomic research in cereals. The purpose of the present study was to design and construct tissue-specific synthetic promoters in rice. We selected several tissue-specific cis-elements based on previous reports and assembled them in different patterns. The combinations were respectively fused to Mini 35S promoter. GUS assays of the transgenic plants indicated that our work successfully created tissue-specific synthetic promoter with the above approach and also revealed that the assembly patterns of cis-elements are critical for the expression patterns and expression efficiencies of synthetic promoters. Our study provides useful information and a practical basis for the design ideas and construction approaches of synthetic promoters in plants.

Key words: Rice, Synthetic promoter, GUS reporter gene, cis-element

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