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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 2094-2098.doi: 10.3724/SP.J.1006.2013.02094

• RESEARCH NOTES • Previous Articles     Next Articles

Molecular Detection and Take-all Response Assays of TiERF1-RC7 Transgenic Wheat

LIU Fei1,YANG Li-Hua1,2,WANG Ai-Yun3,MA Xiao-Fei1,DU Li-Pu2,LIU Xin2,LI Pan-Song1,ZHANG Zeng-Yan2,*,MA Ling-Jian1,*   

  1. 1 Northwest A&F University, Yangling 712100, China; 2 National Key Facility of Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Beijing 100081, China; 3 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
  • Received:2013-03-25 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 张增艳, E-mail: zhangzenmgyan@caas.cn; 马翎健, E-mail: malingjian@nwsuaf.edu.cn

Abstract:

The aim of this study was to understand the role of TiERF1 and RC7 genes in wheat defense response to take-all. Both genes were simultaneously transformed into wheat cultivar Yangmai 18. The resistance to talk-all in five transgenic lines were analyzed by PCR and RT-PCR methods, and evaluated by artificial inoculation in greenhouse and field tests. Scanning electron microscope technique was used to observe the morphology and number of the pathogen mycelia on the root surfaces of resistant transgenic and susceptible receptor wheat plants. The PCR assay showed that both TiERF1 and RC7 genes were integrated into the transgenic lines and inherited to the T3 generation. The RT-PCR assay confirmed expressions of both genes in the transgenic lines. The results showed that five transgenic lines exhibited stable and efficient resistance to take-all with disease severity lower than 10% at seedling stage in greenhouse and rate of white spike lower than 13% at maturity in field. In the contrast of Yangmai18, the severity was 62.98%, rate of white spike was 26.09%. Electron microscope observation revealed that the number and growth tendency of hyphae on roots of the resistant transgenic wheat were significantly lower and weaker than those of the wild type. The expressions of TiERF1 and RC7 inhibit the growth of pathogen hyphae in the transgenic wheat plants, leading to improved resistance to take-all.

Key words: Take-all, Gaeumannomyces graminis var. tritici, Transgenic wheat, Scanning electron microscope

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