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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 2046-2052.doi: 10.3724/SP.J.1006.2011.02046

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Characteristics of Photosynthesis in Transgenic Wheat with Maize C4-PEPC Gene under Field Conditions

WU Qiong1,2,3, XU Wei-Gang2,3,*, LI Yan2,3, QI Xue-Li2,3,HU Lin2,3,ZHANG Lei2,3,HAN Lin-Lin2,3   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Key Laboratory of Wheat Biology, Zhengzhou 450002, China
  • Received:2011-04-01 Revised:2011-06-15 Online:2011-11-12 Published:2011-09-06
  • Contact: 许为钢, E-mail: xuwg1958@sohu.com, Tel: 0371-65712307

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Abstract: Transformation and high efficiency expression of key enzymes gene of C4 photosynthetic characteristics are the important approach to improve photosynthetic efficiency and seed yield for C3 crop wheat. Phosphoenolpyruvate carboxylase (PEPC) plays an important role in C4 pathway. The authors have obtained several transgenic wheat lines with maize C4 type PEPC gene. To explore the expression and functional characteristics of PEPC gene in transgenic wheat plants, PCR test, PEPC activity, gas exchange parameters, chlorophyll fluorescence parameters and yield related characteristics of transgenic wheat lines were determined, using untransformed wheat as the control, and the value of diurnal photosynthesis cumulative (VDPC) at the four stages and the correlations between net photosynthesis rate and stomata conductance, intercellular CO2 concentration and transpiration rate were analyzed. The results showed that in the four determination stages, net photosynthetic rate of transgenic wheat obviously increased For instance VDPC was increased by 29.1% and 23.3% on the 15th day after anthesis. Stomatal conductance and transpiration rate in flag leaves of transgenic wheat obviously increased compared to the control, and intercellular CO2 concentration decreased. Net photosynthetic rate of transgenic wheat enhanced by 23.2%, and variation scope of Fv/Fm, qp, NPQ, and ΦPSII of transgenic wheat was smaller than that of the control from 8:00 to 12:00 on the 15th day after anthesis; the major yield characteristics, such as weight per stem, thousand grain weight, single spike weight and harvest index of transgenic wheat, were significantly higher than those of the control. The results described above indicated that the transgenic wheat expressing maize PEPC gene showed better photosynthetic characteristics than untransformed plant and has the potential to increase wheat yield.

Key words: Phosphoenolpyruvate carboxylase, Transgenic wheat, Photosynthetic characteristics

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