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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 2046-2052.doi: 10.3724/SP.J.1006.2011.02046

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Characteristics of Photosynthesis in Transgenic Wheat with Maize C4-PEPC Gene under Field Conditions

WU Qiong1,2,3, XU Wei-Gang2,3,*, LI Yan2,3, QI Xue-Li2,3,HU Lin2,3,ZHANG Lei2,3,HAN Lin-Lin2,3   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Key Laboratory of Wheat Biology, Zhengzhou 450002, China
  • Received:2011-04-01 Revised:2011-06-15 Online:2011-11-12 Published:2011-09-06
  • Contact: 许为钢, E-mail: xuwg1958@sohu.com, Tel: 0371-65712307

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Abstract: Transformation and high efficiency expression of key enzymes gene of C4 photosynthetic characteristics are the important approach to improve photosynthetic efficiency and seed yield for C3 crop wheat. Phosphoenolpyruvate carboxylase (PEPC) plays an important role in C4 pathway. The authors have obtained several transgenic wheat lines with maize C4 type PEPC gene. To explore the expression and functional characteristics of PEPC gene in transgenic wheat plants, PCR test, PEPC activity, gas exchange parameters, chlorophyll fluorescence parameters and yield related characteristics of transgenic wheat lines were determined, using untransformed wheat as the control, and the value of diurnal photosynthesis cumulative (VDPC) at the four stages and the correlations between net photosynthesis rate and stomata conductance, intercellular CO2 concentration and transpiration rate were analyzed. The results showed that in the four determination stages, net photosynthetic rate of transgenic wheat obviously increased For instance VDPC was increased by 29.1% and 23.3% on the 15th day after anthesis. Stomatal conductance and transpiration rate in flag leaves of transgenic wheat obviously increased compared to the control, and intercellular CO2 concentration decreased. Net photosynthetic rate of transgenic wheat enhanced by 23.2%, and variation scope of Fv/Fm, qp, NPQ, and ΦPSII of transgenic wheat was smaller than that of the control from 8:00 to 12:00 on the 15th day after anthesis; the major yield characteristics, such as weight per stem, thousand grain weight, single spike weight and harvest index of transgenic wheat, were significantly higher than those of the control. The results described above indicated that the transgenic wheat expressing maize PEPC gene showed better photosynthetic characteristics than untransformed plant and has the potential to increase wheat yield.

Key words: Phosphoenolpyruvate carboxylase, Transgenic wheat, Photosynthetic characteristics

[1]Ku M S B, Agarie S, Nomura M, Fukayama H, Tsuchida H, Ono K, Hirose S, Toki S, Miyao M, Matsuoka M. High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants. Nat Biotech, 1999, 17: 76-80
[2]Jeanneau M, Vidal J, Gousset Dupont A, Lebouteiller B, Hodges M, Gerentes D, Perez P. Manipulating PEPC levels in plants. J Exp Bot, 2002, 53: 1837-1845
[3]Von Caemmerer S. C4 photosynthesis in a single C3 cell is theoretically inefficient but may ameliorate internal CO2 diffusion limitations of C3 leaves. Plant Cell & Environ, 2003, 26: 1191-1197
[4]Hibberd J M, Sheehy J E, Langdale J A. Using C4 photosynthesis to increase the yield of rice: rationale and feasibility. Curr Opin Plant Biol, 2008, 11: 228-231
[5]Wu M(吴梅), Zhang B-J(张边江), Chen Q-Z(陈全战), Wang R-F(王荣富). Research progress on introducing efficient photosynthetic C4 genes into C3 plant. Chin Agric Sci Bull (中国农学通报), 2010, 26(3): 68-71 (in Chinese with English abstract)
[6]Veronika D, Helena R. What can enzymes of C4 photosynthesis do for C3 plants under stress? Plant Sci, 2011, 180: 575-583
[7]Zhang G-F(张桂芳), Zhao M (赵明), Ding Z-S(丁在松), Zhang L(张丽), Xiao J-T(肖俊涛). Cloning and characterization of phosphoenolpyruvate carboxylase gene from Echinochloa crusgalli. Acta Agron Sin (作物学报), 2005, 31(10): 1365-1369 (in Chinese with English abstract)
[8]Zhang J F, Bandyopadhyay A, Krisnan S, Wang G Y, Xie H A, Datta K, Datta S K. Characterization of a C4 maize pyruvate orthophosphate dikinase expressed in C3 transgenic rice plants. Afr J Biotechnol, 2010, 9: 234-242
[9]Wang J M, Li R Z. Integration of C4-specific ppdk gene of Echinochloa to C3 upland rice and its photosynthesis characteristics analysis. Afr J Biotechnol, 2008, 7: 783-787
[10]Tsuchida H, Tamai T, Fukayama H, Agarie S, Nomura M, Onodera H, Ono K, Nishizawa Y, Lee B H, Hirose S, Toki S, Ku M S B, Matsuoka M, Miyao M. High level expression of C4-specific NADP-malic enzyme in leaves and impairment of photoautotrophic growth in a C3 plant, rice. Plant Cell Physiol, 2001, 42: 138-145
[11]Chi W, Zhou J S, Zhang F, Wu N H. Photosynthetic features of transgenic rice expressing sorghum C4 type NADP-ME. Acta Bot Sin, 2004, 46: 873-882
[12]Fahnenstich H, Saigo M, Niessen M, Zanor M I, Andreo C S, Fernie A R, Drincovich M F, Flügge U I, Maurino V G. Alteration of organic acid metabolism in Arabidopsis overexpressing the maize C4 NADP-malic enzyme causes accelerated senescence during extended darkness. Plant Physiol, 2007, 145: 640-652
[13]Ding Z-S(丁在松), Zhao M(赵明), Jing Y-X(荆玉祥), Li L-B(李良璧), Kuang T-Y(匡廷云). Effect of overexpression of maize pepc gene on photosynthesis in transgenic rice plants. Acta Agron Sin (作物学报), 2007, 33(5): 717-722 (in Chinese with English abstract)
[14]Ku M S B, Cho D, Ranade U, Hsu T P, Li X, Jiao D M, Ehleringer J, Miyao M, Matsuoka M. Photosynthetic performance of transgenic rice plants overexpressing maize C4 photosynthesis enzymes. In: Sheehy J, Mitchell P L, Hardy B, eds. Redesign Rice Photosynthesis to Improve Yield. Los Baños, the Philippines and Amsterdam, the Netherlands: Elsevier, 2000. pp 193-204
[15]Jiao D M, Li X, Ji B H. Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition. Photosynthetica, 2005, 43: 501-508
[16]Jiao D-M(焦德茂), Li X(李霞), Huang X-Q(黄雪清), Chi W(迟伟), Kuang T-Y(匡廷云), Ku M S B. Characteristics of photosynthetic CO2 assimilation and chlorophyll fluorescence in transgenic rice plants with PEPC gene. Chin Sci Bull (科学通报), 2001, 46(13): 1080-1084 (in Chinese)
[17]Jiao D-M(焦德茂), Kuang T-Y(匡廷云), Li X(李霞), Qiao Y(巧英), Huang X-Q(黄雪清), Hao N-B(郝乃斌), Bai K-Z(白克智). Physiological characteristics of transgenic PEPC rice plants with primary CO2 concentrating mechanism. Sci China (Ser C) (中国科学C辑), 2003, 33(1): 33-39 (in Chinese)
[18]Ji B-H(季本华), Zhu S-Q(朱素琴), Jiao D-M(焦德茂). Photosynthetic C4 microcycle in transgenic rice plant lines expressing the maize C4 photosynthetic enzymes. Acta Agron Sin (作物学报), 2004, 30(6): 536-543 (in Chinese with English abstract)
[19]Chen C-Q(陈绪清), Zhang X-D(张晓东), Liang R-Q(梁荣奇), Zhang L-Q(张立全), Yang F-P(杨凤萍), Cao M-Q(曹鸣庆). Cloning maize C4 phosphoenolpyruvate carboxylase gene and transformation in wheat. Chin Sci Bull (科学通报), 2004, 48(19): 1976-1982 (in Chinese)
[20]Zhang Q-C(张庆琛), Xu W-G(许为钢), Hu L(胡琳), Li Y(李艳), Zhang L(张磊), Qi X-L(齐学礼). Development of transgenic wheat plants with maize C4-specific pepc gene by particle bombardment. J Triticeae Crops (麦类作物学报), 2010, 30(2): 194-197 (in Chinese with English abstract)
[21]Zhang B(张彬), Ding Z-S(丁在松), Zhang G-F(张桂芳), Shi Y-L(石云鹭), Wang J-M(王金明), Fang L-F(方立锋), Guo Z-J (郭志江), Zhao M(赵明). Introduction of phosphoenolpyruvate carboxylase gene from Echinochloa crusgalli into wheat mediated by Agrobacterium tumefaciens. Acta Agron Sin (作物学报), 2007, 33(3): 356-362 (in Chinese with English abstract)
[22]Li Y(李艳), Xu W-G(许为钢), Hu L(胡琳), Zhang L(张磊), Qi X-L(齐学礼), Zhang Q-C(张庆琛), Wang G-S(王根松). Construction of a high efficient expression vector for maize phosphoenolpyruvate carboxylase gene and its transformation in wheat. J Triticeae Crops (麦类作物学报), 2009, 29(5): 741-746 (in Chinese with English abstract)
[23]Li Y(李艳). Cloning of Photosynthesis Key Enzyme (PEPC, PPDK) Genes from Maize and PEPC Gene Transformation of Wheat. PhD Dissertation of Henan Agricultural University, 2009 (in Chinese with English abstract)
[24]Lin R C, Ding Z S, Li L B, Kuang T Y. A rapid and efficient DNA minipreparation suitable for screening transgenic plants. Plant Mol Biol Rep, 2001, 19: 379a-379e
[25]Jeanneau M, Gerentes D, Foueillassar X, Zivy M, Vidal J, Toppan A, Perez P. Improvement of drought tolerance in maize: towards the functional validation of the Zm-Asr1 gene and increase of water use efficiency by over-expressing C4 PEPC. Biochimie, 2002, 84: 1127-1135
[26]Wang S-Z(王绍中), Tian Y-F(田云峰), Guo T-C(郭天才), Wang Z-H(王志和). Wheat Cultivation in Henan(河南小麦栽培学). Beijing: China Agricultural Science and Technology Press, 2010. pp 17-23 (in Chinese)
[27]Wang L-L(王兰兰), He X-Y(何兴元), Chen W(陈玮), Li X-M(李雪梅). Effects of elevated O3 or/and CO2 on growth in leaves of Quercus mongolia. China Environ Sci (中国环境科学), 2011, 31(2): 340-345 (in Chinese with English abstract)
[28]Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta, 1989, 990: 87-92
[29]Agarie S, Miura A, Sumikura R, Tsukamoto S, Nose A, Arima S, Matsuoka M, Miyao-Tokutomi M. Overexpression of C4 PEPC caused O2-insensitive photosynthesis in transgenic rice plant. Plant Sci, 2002, 162: 257-265
[30]Fukayama H, Hatch M D, Tamai T, Tsuchida H, Sudoh S, Furbank R T, Miyao M. Activity regulation and physiological impacts of maize C4-specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants. Photosyn Res, 2003, 77: 227-239
[31]Taniguchi Y, Ohkawa H, Masumoto C, Fukuda T, Tamai T, Lee K, Sudoh S, Tsuchida H, Sasaki H, Fukayama H, Miyao M. Overproduction of C4 photosynthetic enzymes in transgenic rice plants: an approach to introduce the C4-like photosynthetic path way into rice. J Exp Bot, 2008, 59: 1799-1809
[32]Jiao D M, Huang X Q, Li X, Chi W, Kuang T Y, Zhang Q D, Ku M S B, Cho D. Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes. Photosyn Res, 2002, 72: 85-93
[33]Chi W(迟伟), Jiao D-M(焦德茂), Huang X-Q(黄雪清), Li X(李霞), Ku M S B. Photosynthetic characteristics of transgenic rice plant overexpressing maize phosphoenolpyruvate carboxylase. Acta Bot Sin (植物学报), 2001, 43(6): 657-660 (in Chinese with English abstract)
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