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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1561-1569.doi: 10.3724/SP.J.1006.2012.01561

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Pattern of Arabidopsis RBCS-1A Gene in Response to Light Treatments and Application Evaluation of Its Promoter in Transgenic Engineering

XI Yu-Lin1,2,ZHOU Peng1,SONG Mei-Fang1,LI Zhi-Yong1,3,MENG Fan-Hua1,YANG Jian-Ping1,4,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Agronomy College, Henan Agricultural University, Zhengzhou 450002, China; 4 College of Bio-information, Chongqing University of Posts and Tele-communication, Chongqing 400065, China
  • Received:2012-03-06 Revised:2012-04-20 Online:2012-09-12 Published:2012-07-03
  • Contact: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859

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Abstract: RBCS gene encodes the small subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is very important for crop improvement to study the gene expression pattern and promoter activity of RBCS in response to light. In this study, we analyzed the gene expression pattern of Arabidopsis RBCS-1A in response to light by Real-time quantitative PCR. The results indicated that the expression of AtRBCS-1A was induced by light, and had the tissue-specific feature. In silico analysis revealed that many cis-acting elements for light regulation were found in the promoter of AtRBCS-1A. Based on this information, we isolated a genomic sequence with 1 691 bp upstream of the translation start codon of AtRBCS-1A using PCR, constructed plasmid vector with AtRBCS-1A promoter fused with GUS (encoding β-glucuronidase) reporter gene, and then generated transgenic Arabidopsis by floral dip. Histochemical analysis of the transgenic lines suggested that AtRBCS-1A promoter was a light-induced and tissue-specific promoter. All the analyses preliminarily prove that AtRBCS-1A promoter has important application value and can be used in plant transgenic engineering.

Key words: Arabidopsis, RBCS-1A gene, Expression pattern, Light-induced expression, cis-acting elements

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