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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2206-2216.doi: 10.3724/SP.J.1006.2012.02206


Establishment of Molecular ID of Soybean Varieties (Lines) with SSR Marker Linked to Resistance Genes of Cercospora sojina

DING Jun-Jie1,JIANG Cui-Lan2,GU Xin1,YANG Xiao-He1,ZHAO Hai-Hong1,SHEN Hong-Bo1,SHI Xiang-Lin3,LIU Chun-Yan3,HU Guo-Hua3,*,CHEN Qing-Shan4,*   

  1. 1 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences / Observation and Experiment Station of Crop Pests of Jiamusi, Ministry of Agriculture, Jiamusi 154007, China; 2 Institute of Crop, Heilongjiang Academy of Land Reclamation Science, Jiamusi 154007, China; 3 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 4 College of Agriculture, Northeast Agricultural University, Harbin 150030, China
  • Received:2012-02-22 Revised:2012-08-05 Online:2012-12-12 Published:2012-10-08
  • Contact: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@sohu.com


One hundred and three soybean varieties (lines) developed by 29 breeding units from six accumulated temperature zones in Heilongjiang were investigated. The resistances of 103 soybean varieties (lines) were identified with three races of Cercospora sonija. Eighty-six polymorphic alleles were detected with 19 SSR markers, for each primer, two to six allele variations were detected in all varieties (lines), with an average of 4.42. The diversity index of the 19 SSR loci ranged from 0.198 to 0.751 with an average of 0.606. The results of calculating specific index and genetic similarity among varieties (lines) showed that specific index of varieties (lines) ranged from 46.592 to 481.541, and the average was 87.415. According to fragment size of allele variation, the data calculated from the PAGE bands were analyzed by the software ID Analysis 1.0. Seven markers (Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, and Sat_151) linked to the gene controlling resistance to Cercospora sojina were used to identify all 103 soybean lines, so that a set of molecular ID of soybean varieties (lines) was established.

Key words: Soybean frogeye leaf spot, Physiological race, SSR, Molecular ID

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