Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2286-2296.doi: 10.3724/SP.J.1006.2012.02286

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity and Population Structure of Germplasm Resources in Sesame (Sesamum indicum L.) by SSR Markers

YUE Wen-Di1,WEI Li-Bin2,ZHANG Ti-De2,LI Chun2,MIAO Hong-Mei2,ZHANG Hai-Yang2,*   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China; 2 Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2012-04-24 Revised:2012-09-05 Online:2012-12-12 Published:2012-10-08
  • Contact: 张海洋, E-mail: zhy@hnagri.org.cn, Tel: 0371-65715936

RichHTML

PDF

1904

Cited

7

Abstract:

A total of 545 sesame accessions, including domestic and foreign germplasm resources, were analyzed systematically with 42 SSR primer pairs. The results showed that 106 allele bands were detected among the sesame accessions, and the Shannon’s information index (I) was 0.4092. Analysis results of UPGMA clustering, two-dimension principal components and population structure were basically consistent; 545 varieties were divided into three groups in UPGMA dentrogram or three subgroups in population structure. There were the lower genetic differentiation coefficient and the closer sibship in worldwide sesame. The similarity coefficient of seven ecological groups in China ranged from 0.9811 to 0.5462, and there was no close genetic relationship between varieties from northern regions and other regions in China. The similarity coefficients for foreign varieties were from 0.9726 to 0.7442, and the genetic relationships of varieties from Africa zone was close to those from Japan zone, but not from China. It showed that the genetic basis of varieties from China was relatively narrow, and the genetic diversities of varieties were not completely related to geopraphic distribution, but the genetic diversities of varieties from abroad presented abundance. Therefore, the introduction of foreign resources should be reinforced, and the domestic resources from different regions should be utilized in the future sesame breeding.

Key words: Sesamum indicum.L, Germplasm resource, SSR marker, Genetic diversity, Population structure

[1]Bedigian D, Harlan J. Evidence for cultivation on sesame in the ancient world. Econ Bot, 1986, 40: 137–154



[2]Ashri A. Sesame breeding. Plant Breed Rev, 1998, 16: 179–228



[3]Bisht I S, Mahajan R K, Loknathan T R, Agrawal R C. Diversity in Indian sesame collection and stratification of germplasm accessions in different diversity groups. Genet Resour Crop Evol, 1998, 45: 325–335



[4]Shen J-X(沈金雄), Guo Q-Y(郭庆元), Zhang X-R(张秀荣), Zhao Y-Z(赵应忠), Feng X-Y(冯祥运), Chen H-X(陈和兴), Wu X-M(伍晓明). Cluster analysis of sesame germplasm collection in China. Huazhong Agric Univ (华中农业大学学报), 1995, 14(6): 532–536 (in Chinese with English abstract)



[5]Shiro I, Teruhisa U. Genetic variations of isozymes in cultivated sesame (Sesamum indicum L.). Euphytica, 1997, 93: 375–377



[6]Zhao Y-Z(赵应忠), Liu H-Y(刘红艳). Genetic distance and heterosis between male sterile lines and core collection in sesame. Chin J Oil Crop Sci (中国油料作物学报), 2005, 27(1): 36–40 (in Chinese with English abstract)



[7]Zhang P(张鹏), Zhang H-Y(张海洋), Zheng Y-Z(郑永战), Guo W-Z(郭旺珍), Wei L-B(魏利斌). Factor and cluster analysis of sesame (Sesamum indicum L.) germplasm resources. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(1): 71–78 (in Chinese with English abstract)



[8]Zhang X-R(张秀荣), Chen K-R(陈坤荣), Peng J(彭俊), Xu Z-Y(许泽永). The RAPD analysis and genetic diversity of selected sesame germplasms. Chin J Oil Crop Sci (中国油料作物学报), 2004, 26(4): 34–37 (in Chinese with English abstract)



[9]Gulhan E A, Melih T, Kenan T. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol, 2004, 51: 599–607



[10]Che Z(车卓), Zhang Y-X(张艳欣), Sun J(孙建), Zhang X-R(张秀荣), Shang X-W(尚勋武), Wang H-J(王化俊). Genetic diversity analysis of black sesame (Sesamum indicum DC.) core collection of China using SRAP markers. Acta Agron Sin (作物学报), 2009, 35(10): 1936–1941 (in Chinese with English abstract)



[11]Cho Y, Park J. Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L) using microsatellite markers. Genes & Genom, 2011, 33: 187–195



[12]Kim D H, Zur G, Danin P Y, Lee S W, Shim K B, Kang C W, Kashi Y. Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. Plant Breed, 2002, 121: 259–262



[13]Hernán E L, Petr K. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genet, 2006, 7: 10



[14]Wei L B, Zhang H Y, Zheng Y Z, Miao H M, Zhang T Z, Guo W Z. A genetic linkage map construction for sesame (Sesamum indicum L.). Genes & Genom, 2009, 31(2): 199–208



[15]Chen C-Y(陈翠云), Feng X-Y(冯祥运). Sesame Germplasm Resources in China (中国芝麻品种志). Beijing: Agriculture Press, 1990. pp 1–17 (in Chinese)



[16]Paterson A H, Brubaker C, Wendel J F. A rapid method for extraction of cotton (Gossypium spp) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol, 1999, 11: 122–127



[17]Liu K J, Muse S V. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21: 2128–2129



[18]Pritchard J K, Stephens M, Donnelly P.2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945–959



[19]Rohlf F J. NTSYSpc: Numerical Taxonomy System, Ver.2.20. Setauket, NY, USA: Exeter Publishing Ltd., 2008



[20]Yeh F C, Boyle T J B. Population genetic analysis of codominant and dominant markers and quantitative traits. Belg J Bot, 1997, 129: 157



[21]Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24: 1596–1599



[22]Xiao J, Li J, Yuan L, McCouch S R, Tanksley S D. Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers, Theor Appl Genet. 1996, 92: 637–643



[23]Liu Z W, Biyashev R M, Saghai Maroof M A. Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet, 1996, 93: 869–876



[24]McCouch S R, Lu H, Rutger J N, Coburn J R, Tai T H. Population structure and breeding patterns of 145 US rice cultivars based on SSR marker analysis, Crop Sci, 2005, 45: 66–76



[25]Zhang P(张鹏), Zhang H-Y(张海洋), Guo W-Z(郭旺珍), Zheng Y-Z(郑永战), Wei L-B(魏利斌), Zhang T-Z(张天真). Genetic diversity analysis of Sesamum indicum L. germplasms using SRAP and SSR markers. Acta Agron Sin (作物学报), 2007, 33(10): 1696–1702 (in Chinese with English abstract)



[26]Zhang Y-X(张艳欣), Zhang X-R(张秀荣), Che Z(车卓), Wang L-H(王林海). Genetic diversity analysis of core collection of white coat sesame seed (Sesamum indicum L.) in China using SRAP markers. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32(1): 46–52 (in Chinese with English abstract)



[27]Laurentin H E, Karlovsky P. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genet, 2006, 7: 10



[28]Erca A G, Taskin M, Turgut K. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol, 2004, 51: 599–607
[1] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[2] Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[3] Xu Jian-Xia, Ding Yan-Qing, Cao Ning, Cheng Bin, Gao Xu, Li Wen-Zhen, Wang Ruo-Ruo, Wang Lei, Zhang Li-Yi. Phenotypic diversity analysis and comprehensive evaluation of 397 sorghum germplasm resources in Guizhou, China [J]. Acta Agronomica Sinica, 2026, 52(4): 1073-1087.
[4] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[5] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
[6] Li Shi-Qing, Wang Qian, Wang Su-Hua, Zhang Yao-Wen, Wang Li-Xia. Evaluation of salt tolerance at the seedling stage and related gene mining in mung bean germplasm resources [J]. Acta Agronomica Sinica, 2026, 52(2): 376-388.
[7] MEI Piao, LIU Ding-Ding, YE Yuan-Yuan, ZHANG Chen-Yu, DING Shi-Qi, LI Ya-Qi, WANG Pei-Xin, MEI Ju-Fen, MA Chun-Lei. Genetic diversity analysis of domestic albino tea germplasm resources based on the tea plant liquid phase functional chip [J]. Acta Agronomica Sinica, 2025, 51(9): 2358-2370.
[8] WANG Tian-Yi, YANG Xiu-Juan, ZHAO Jia-Jia, HAO Yu-Qiong, ZHENG Xing-Wei, WU Bang-Bang, LI Xiao-Hua, HAO Shui-Yuan, ZHENG Jun. Gliadin diversity and its effects on flour quality in wheat from Shanxi province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1784-1800.
[9] LIANG Hong-Kai, ZHAO Su-Meng, LU Qiong, ZHOU Peng, ZHI Hui, DIAO Xian-Min, HE Qiang. A mini-core collection of foxtail millet [J]. Acta Agronomica Sinica, 2025, 51(6): 1435-1444.
[10] WANG Mu, ZHUO Ga, ZHA Sang, XIRUO Qu-Zong, DAWA Dondup, GUO Gang-Gang, ZHANG Jing, ZHUO Ga, LHUNDRUP Namgyal. Genetic diversity analysis and comprehensive evaluation of Qingke germplasm based on six phenotypic traits [J]. Acta Agronomica Sinica, 2025, 51(6): 1526-1537.
[11] WANG Run-Feng, LI Wen-Jia, LIAO Yong-Jun, LU Qing, LIU Hao, LI Hai-Fen, LI Shao-Xiong, LIANG Xuan-Qiang, HONG Yan-Bin, CHEN Xiao-Ping. Evaluation of pod maturity and identification of early-maturing germplasm for core peanut germplasm resources [J]. Acta Agronomica Sinica, 2025, 51(2): 395-404.
[12] SUN Xian-Jun, YU Tai-Fei, HU Zheng, SHEN Xin-Ping, GE Wen-Yi, JIANG Xue-Min, WANG Shi-Jia, YU Si-Jia, WU Shu-Yu, HAN Long-Zhi, ZHANG Hui, JIANG Qi-Yan. Assessment of salt-alkali tolerance throughout the rice growth period and germplasm screening based on the coefficient of standard deviation weighting method [J]. Acta Agronomica Sinica, 2025, 51(12): 3369-3376.
[13] TANG Yong-Yan, YIN Jun-Jie, HOU Qing-Qing, FENG Jun-Yan, LI Jun, YANG Wu-Yun, CHEN Xue-Wei, HU Pei-Song, WAN Jian-Min. Current status and countermeasures for crop seed industry development in Sichuan province, China [J]. Acta Agronomica Sinica, 2025, 51(11): 2845-2859.
[14] GE Jia-Hao, LEI Xin-Yue, WANG Qing-Ming, HAN Hui-Bing, LI Shao-Fei, WANG Qi-Xuan, FENG Bai-Li, GAO Jin-Feng. Screening of low-phosphorus tolerant germplasm and comprehensive evaluation of low phosphorus tolerance in Tartary buckwheat at seedling stage [J]. Acta Agronomica Sinica, 2025, 51(11): 2911-2922.
[15] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd