Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2286-2296.doi: 10.3724/SP.J.1006.2012.02286

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity and Population Structure of Germplasm Resources in Sesame (Sesamum indicum L.) by SSR Markers

YUE Wen-Di1,WEI Li-Bin2,ZHANG Ti-De2,LI Chun2,MIAO Hong-Mei2,ZHANG Hai-Yang2,*   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China; 2 Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2012-04-24 Revised:2012-09-05 Online:2012-12-12 Published:2012-10-08
  • Contact: 张海洋, E-mail: zhy@hnagri.org.cn, Tel: 0371-65715936

RichHTML

PDF

1499

Cited

7

Abstract:

A total of 545 sesame accessions, including domestic and foreign germplasm resources, were analyzed systematically with 42 SSR primer pairs. The results showed that 106 allele bands were detected among the sesame accessions, and the Shannon’s information index (I) was 0.4092. Analysis results of UPGMA clustering, two-dimension principal components and population structure were basically consistent; 545 varieties were divided into three groups in UPGMA dentrogram or three subgroups in population structure. There were the lower genetic differentiation coefficient and the closer sibship in worldwide sesame. The similarity coefficient of seven ecological groups in China ranged from 0.9811 to 0.5462, and there was no close genetic relationship between varieties from northern regions and other regions in China. The similarity coefficients for foreign varieties were from 0.9726 to 0.7442, and the genetic relationships of varieties from Africa zone was close to those from Japan zone, but not from China. It showed that the genetic basis of varieties from China was relatively narrow, and the genetic diversities of varieties were not completely related to geopraphic distribution, but the genetic diversities of varieties from abroad presented abundance. Therefore, the introduction of foreign resources should be reinforced, and the domestic resources from different regions should be utilized in the future sesame breeding.

Key words: Sesamum indicum.L, Germplasm resource, SSR marker, Genetic diversity, Population structure

[1]Bedigian D, Harlan J. Evidence for cultivation on sesame in the ancient world. Econ Bot, 1986, 40: 137–154



[2]Ashri A. Sesame breeding. Plant Breed Rev, 1998, 16: 179–228



[3]Bisht I S, Mahajan R K, Loknathan T R, Agrawal R C. Diversity in Indian sesame collection and stratification of germplasm accessions in different diversity groups. Genet Resour Crop Evol, 1998, 45: 325–335



[4]Shen J-X(沈金雄), Guo Q-Y(郭庆元), Zhang X-R(张秀荣), Zhao Y-Z(赵应忠), Feng X-Y(冯祥运), Chen H-X(陈和兴), Wu X-M(伍晓明). Cluster analysis of sesame germplasm collection in China. Huazhong Agric Univ (华中农业大学学报), 1995, 14(6): 532–536 (in Chinese with English abstract)



[5]Shiro I, Teruhisa U. Genetic variations of isozymes in cultivated sesame (Sesamum indicum L.). Euphytica, 1997, 93: 375–377



[6]Zhao Y-Z(赵应忠), Liu H-Y(刘红艳). Genetic distance and heterosis between male sterile lines and core collection in sesame. Chin J Oil Crop Sci (中国油料作物学报), 2005, 27(1): 36–40 (in Chinese with English abstract)



[7]Zhang P(张鹏), Zhang H-Y(张海洋), Zheng Y-Z(郑永战), Guo W-Z(郭旺珍), Wei L-B(魏利斌). Factor and cluster analysis of sesame (Sesamum indicum L.) germplasm resources. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(1): 71–78 (in Chinese with English abstract)



[8]Zhang X-R(张秀荣), Chen K-R(陈坤荣), Peng J(彭俊), Xu Z-Y(许泽永). The RAPD analysis and genetic diversity of selected sesame germplasms. Chin J Oil Crop Sci (中国油料作物学报), 2004, 26(4): 34–37 (in Chinese with English abstract)



[9]Gulhan E A, Melih T, Kenan T. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol, 2004, 51: 599–607



[10]Che Z(车卓), Zhang Y-X(张艳欣), Sun J(孙建), Zhang X-R(张秀荣), Shang X-W(尚勋武), Wang H-J(王化俊). Genetic diversity analysis of black sesame (Sesamum indicum DC.) core collection of China using SRAP markers. Acta Agron Sin (作物学报), 2009, 35(10): 1936–1941 (in Chinese with English abstract)



[11]Cho Y, Park J. Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L) using microsatellite markers. Genes & Genom, 2011, 33: 187–195



[12]Kim D H, Zur G, Danin P Y, Lee S W, Shim K B, Kang C W, Kashi Y. Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. Plant Breed, 2002, 121: 259–262



[13]Hernán E L, Petr K. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genet, 2006, 7: 10



[14]Wei L B, Zhang H Y, Zheng Y Z, Miao H M, Zhang T Z, Guo W Z. A genetic linkage map construction for sesame (Sesamum indicum L.). Genes & Genom, 2009, 31(2): 199–208



[15]Chen C-Y(陈翠云), Feng X-Y(冯祥运). Sesame Germplasm Resources in China (中国芝麻品种志). Beijing: Agriculture Press, 1990. pp 1–17 (in Chinese)



[16]Paterson A H, Brubaker C, Wendel J F. A rapid method for extraction of cotton (Gossypium spp) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol, 1999, 11: 122–127



[17]Liu K J, Muse S V. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21: 2128–2129



[18]Pritchard J K, Stephens M, Donnelly P.2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945–959



[19]Rohlf F J. NTSYSpc: Numerical Taxonomy System, Ver.2.20. Setauket, NY, USA: Exeter Publishing Ltd., 2008



[20]Yeh F C, Boyle T J B. Population genetic analysis of codominant and dominant markers and quantitative traits. Belg J Bot, 1997, 129: 157



[21]Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24: 1596–1599



[22]Xiao J, Li J, Yuan L, McCouch S R, Tanksley S D. Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers, Theor Appl Genet. 1996, 92: 637–643



[23]Liu Z W, Biyashev R M, Saghai Maroof M A. Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet, 1996, 93: 869–876



[24]McCouch S R, Lu H, Rutger J N, Coburn J R, Tai T H. Population structure and breeding patterns of 145 US rice cultivars based on SSR marker analysis, Crop Sci, 2005, 45: 66–76



[25]Zhang P(张鹏), Zhang H-Y(张海洋), Guo W-Z(郭旺珍), Zheng Y-Z(郑永战), Wei L-B(魏利斌), Zhang T-Z(张天真). Genetic diversity analysis of Sesamum indicum L. germplasms using SRAP and SSR markers. Acta Agron Sin (作物学报), 2007, 33(10): 1696–1702 (in Chinese with English abstract)



[26]Zhang Y-X(张艳欣), Zhang X-R(张秀荣), Che Z(车卓), Wang L-H(王林海). Genetic diversity analysis of core collection of white coat sesame seed (Sesamum indicum L.) in China using SRAP markers. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32(1): 46–52 (in Chinese with English abstract)



[27]Laurentin H E, Karlovsky P. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genet, 2006, 7: 10



[28]Erca A G, Taskin M, Turgut K. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol, 2004, 51: 599–607
[1] XIAO Ying-Ni, YU Yong-Tao, XIE Li-Hua, QI Xi-Tao, LI Chun-Yan, WEN Tian-Xiang, LI Gao-Ke, HU Jian-Guang. Genetic diversity analysis of Chinese fresh corn hybrids using SNP Chips [J]. Acta Agronomica Sinica, 2022, 48(6): 1301-1311.
[2] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[3] HU Liang-Liang, WANG Su-Hua, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage [J]. Acta Agronomica Sinica, 2022, 48(2): 367-379.
[4] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[5] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[6] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[7] SUN Qian, ZOU Mei-Ling, ZHANG Chen-Ji, JIANG Si-Rong, Eder Jorge de Oliveira, ZHANG Sheng-Kui, XIA Zhi-Qiang, WANG Wen-Quan, LI You-Zhi. Genetic diversity and population structure analysis by SNP and InDel markers of cassava in Brazil [J]. Acta Agronomica Sinica, 2021, 47(1): 42-49.
[8] SUN Zhi-Guang, WANG Bao-Xiang, ZHOU Zhen-Ling, FANG Lei, CHI Ming, LI Jing-Fang, LIU Jin-Bo, Bello Babatunde Kazeem, XU Da-Yong. Screening of germplasm resources and QTL mapping for germinability under submerged condition in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(1): 61-70.
[9] Meng-Liang ZHAO,Li-Hui WANG,Yan-Jing REN,Xue-Mei SUN,Zhi-Qiang HOU,Shi-Peng YANG,Li LI,Qi-Wen ZHONG. Genetic diversity of phenotypic traits in 257 Jerusalem artichoke accessions [J]. Acta Agronomica Sinica, 2020, 46(5): 712-724.
[10] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[11] MA Yan-Ming, FENG Zhi-Yu, WANG Wei, ZHANG Sheng-Jun, GUO Ying, NI Zhong-Fu, LIU Jie. Genetic diversity analysis of winter wheat landraces and modern bred varieties in Xinjiang based on agronomic traits [J]. Acta Agronomica Sinica, 2020, 46(12): 1997-2007.
[12] MA Yan-Ming, LOU Hong-Yao, CHEN Zhao-Yan, XIAO Jing, XU Lin, NI Zhong-Fu, LIU Jie. Genetic diversity assessment of winter wheat landraces and cultivars in Xinjiang via SNP array analysis [J]. Acta Agronomica Sinica, 2020, 46(10): 1539-1556.
[13] LIU Yi-Ke,ZHU Zhan-Wang,CHEN Ling,ZOU Juan,TONG Han-Wen,ZHU Guang,HE Wei-Jie,ZHANG Yu-Qing,GAO Chun-Bao. Revealing the genetic diversity of wheat varieties (lines) in China based on SNP markers [J]. Acta Agronomica Sinica, 2020, 46(02): 307-314.
[14] ZHANG Xiao-Jun,XIAO Jin,WANG Hai-Yan,QIAO Lin-Yi,LI Xin,GUO Hui-juan,CHANG Li-Fang,ZHANG Shu-Wei,YAN Xiao-Tao,CHANG Zhi-Jian,WU Zong-Xin. Evaluation of resistance to Fusarium head blight in Thinopyrum-derived wheat lines [J]. Acta Agronomica Sinica, 2020, 46(01): 62-73.
[15] GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui. Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus [J]. Acta Agronomica Sinica, 2019, 45(9): 1416-1430.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd