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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 649-656.doi: 10.3724/SP.J.1006.2013.00649

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of Saccharum spontaneum L. Core Collections

QI Yong-Wen,FAN Li-Na,LUO Qing-Wen,WANG Qin-Nan,CHEN Yong-Sheng,HUANG Zhong-Xing,LIU Rui,LIU Shao-Mou,DENG Hai-Hua*,LI Qi-Wei*   

  1. Guangdong Key Laboratory of Sugarcane Improvement and Biorefine, Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China
  • Received:2012-09-18 Revised:2012-12-12 Online:2013-04-12 Published:2013-01-28
  • Contact: 李奇伟, E-mail: liqiwei66@163.com, Tel: 020-84168478; 邓海华, E-mail: haihuadeng@126.com, Tel: 020-84178327

Abstract:

Five hundred and forty accessions of Saccharum spontaneum L. from ten provinces of China were characterized by geographical information, molecular markers on 20 SSRs and 15 agro-morphological traits to establish a core collection. Ten different sampling ratios of accessions were tested, including 5% and 10% to 90% with an interval of 10%. The results showed that 10% of samples could conserve more than 70% of variations, which indicated that 10% was an adequate sampling ratio for establishing the core collection. A comparison of the efficiencies of five sampling strategies by geographical grouping (constant, proportional, square root, logarithmic, and genetic diversity-depend) and two non-grouping sampling strategies (maximization and random sampling), indicated proportional strategy was optimal in term of establishing the most representative core collection. Thus, a core collection of 60 accessions, including 54 selected based on proportional strategy and six accessions selected by their specific agro-morphological traits, was constructed. The tests on SSR data and agro-morphological characters demonstrated that the core collection have high genetic diversity and a good representative to the entire collection.

Key words: Saccharum spontaneum L., Core collection, SSR, Agro-morphological traits

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