Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 476-486.doi: 10.3724/SP.J.1006.2014.00476

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

An Efficient Approach to Identify Salt Tolerance of Upland Cotton at Seedling Stage

PENG Zhen,HE Shou-Pu,SUN Jun-Ling,XU Fei-Fei,JIA Yin-Hua,PAN Zhao-E,WANG Li-RuDU Xiong-Ming*   

  1. Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China
  • Received:2013-08-06 Revised:2013-12-05 Online:2014-03-12 Published:2014-01-16
  • Contact: 杜雄明, E-mail: dxm630723@163.com, Tel: 0372-2562252

Abstract:

Three-leaf cotton seedlings of two salt-tolerant varieties and two salt-sensitive varieties were treated by water and 4% (40 g L–1) NaCl solution, respectively. A total of 13 parameters related to salt tolerance including salt injury index (SII), shoot fresh weight (SRW), root fresh weight (RFW), leaf relative water content (RWC), chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm), relative conductivity (RC), manlondialdehyde (MDA) content and the activity of antioxidant enzymes were monitored after 72h of treatment. Comprehensive assessment of salinity tolerance based on grey relation clustering method, principal component analysis and stepwise regression analysis indicated that themaximum quantum yield of PSII was the most significant correlated indices with salt tolerance in upland cotton, which could be used as a single parameter to assess salt tolerance based on the equation: y = 1.943x – 0.882 (where y is the salt tolerance index, x is the related value of maximum efficiency of photosystem II). The other two salt-tolerant varieties and two salt-sensitive varieties were used to rate the classification of salt tolerance. Salt tolerance index (y) of 23 varieties with known salt tolerance were calculated to validate the accuracy of the equation, the result was consistent with field investigation. In this study, the maximum efficiency of photosystem II was used as the main index to validate the salt tolerance in cotton, together with the construction of salt tolerance index equation and salt tolerance rating, which could greatly improve the efficiency of salinity tolerance evaluation for massive germplasm in future.

Key words: Upland cotton, Seedling stage, Salt tolerance evaluation, Maximum quantum yield of PSII, Index of salinity tolerance

[1]黄滋康, 季道藩, 潘家驹. 中国棉花遗传育种学. 济南: 山东科学技术出版社, 2003. pp 533–544



Huang Z K, Ji D F, Pan J J. Genetics and Breeding of Cotton in China. Jinan: Shandong Science and Technology Press, 2003. pp 533–544 (in Chinese)



[2]张国伟, 路海玲, 张雷, 陈兵林, 周治国.棉花萌发期和苗期耐盐性评价及耐盐指标筛选. 应用生态学报, 2011, 22: 2045–2053



Zhang G W, Lu H L, Zhang L, Chen B L, Zhou Z G. Salt tolerance evaluation of cotton (Gossypium hirsutum L.) at its germinating and seedling stages and selection of related indices. Chin J Appl Ecol, 2011, 22: 2045–2053 (in Chinese with English abstract)



[3]辛承松, 董合忠, 唐薇, 温四民. 棉花盐害与耐盐性的生理和分子机制研究进展. 棉花学报, 2005, 17: 309–313



Xin C S, Dong H Z, Tang W, Wen S M. Physiological and molecular mechanisms of salt injury and salt tolerance in cotton. Cotton Sci, 2005, 17: 309–313 (in Chinese with English abstract)



[4]Basal H. Response of cotton (Gossypium hirsutum L.) genotypes to salt stress. Pak J Bot, 2010, 42: 505–511



[5]Basel S. Salt stress alters physiological indicators in cotton (Gossypium hirsutum L.). Soil Environ, 2012, 31:113–118



[6]Higbie S M, Wang F, Stewart M D, Sterling T M, LindemannW C, Hughs E, Zhang J F. Physiological response to salt (NaCl) stress in selected cultivated tetraploid cottons. Intl J Agron, 2010, 1: 1–10



[7]Shaheen H L, Shahbaz M. Salt-induced effects on some key morpho-physiological attributes of cotton (Gossypium hirsutum L.) at various growth stages. Soil Environ, 2012, 31: 125–133



[8]孙小芳, 刘友良. 棉花品种耐盐性鉴定指标可靠性的检验. 作物学报, 2001, 27: 794–796



SunXF, Liu YL.Test on criteria of evaluating salt tolerance of cotton cultivars. Acta Agron Sin, 2001, 27: 794–796 (in Chinese with English abstract)



[9]李寒暝, 白灯莎•买买提艾力, 张少民, 阿依夏木•沙吾尔, 蒋平安. 新疆棉花品种的耐盐性综合评价. 核农学报, 2010, 24: 160–165



Li H M, Baidengsha M M T A L, Zhang S M, A Yixiamu S W E, Jiang P A. Evaluation of salt resistance of seven cotton (Gossypium hirsutum L.) varieties in Xinjiang. J Nucl Agric Sci, 2010, 24: 160–165 (in Chinese with English abstract)



[10]刘国强, 香黎明, 刘金定. 棉花品种资源耐盐性鉴定研究. 作物品种资源, 1993, 2: 21



Liu G Q, Lu L L, Liu J D. Salt tolerance of cotton germplasm identification. Crop Genet Resour, 1993, 2: 21 (in Chinese)



[11]张保青, 杨丽涛, 李杨瑞. 自然条件下甘蔗品种抗寒生理生化特性的比较. 作物学报, 2011, 37: 496–505



Hang B Q, Yang L T, Li Y R. Comparison of physiological and biochemical characteristics related to cold resistance in sugarcane under field conditions. Acta Agron Sin, 2011, 37: 496–505 (in Chinese with English abstract)



[12]刘少卿, 何守朴, 米拉吉古丽, 周忠丽, 孙君灵, 杜雄明. 不同棉花种质资源耐热性鉴定. 植物遗传资源学报, 2013, 14: 219–220



Liu S Q, He S P, Milajiguli, Zhou Z L, Sun J L, Du X M. Identification for the thermotolerance of different germplasm in cotton. J Plant Genet Resour, 2013, 14: 214–221 (in Chinese with English abstract)



[13]胡标林, 扬平, 万勇, 李霞, 罗世友, 罗向东, 谢建坤. 东乡野生稻BILs群体苗期抗寒性综合评价及其遗传分析. 植物遗传资源学报, 2013, 14: 249–255



Hu BL, Yang P, Wan Y, Li X, Luo S Y, LuoX D, Xie J K. Comprehensive assessment of drought resistance of BILs population derived from Dongxiang wild rice (Oryza rufupogon Griff.) at seedling stage and its genetic analysis. J Plant Genet Resour, 2013, 14: 249–256 (in Chinese with English abstract)



[14]胡标林, 余守武, 万勇, 张铮, 邱兵余, 谢建坤. 东乡普通野生稻全生育期抗旱性鉴定. 作物学报, 2007, 33:425–432



Hu B L, Yu S W, Wan Y, Zhang Z, Qiu B Y, Xie J K. Drought-resistance identification of dongxiang common wild rice (Oryza rufipogon Griff.) in whole growth period. Acta Agron Sin, 2007, 33: 425–432 (in Chinese with English abstract)



[15]孙晓东, 胡劲松, 焦玥. 基于主成分分析和灰色关联聚类分析的指标综合方法研究. 中国管理科学, 2005, 13: 18–22



SunX D, Hu J S, Jiao Y. Research on index integration method based on principal component analysis and grey relation clustering analysis. Chin J Manag Sci, 2005, 13: 18–22 (in Chinese with English abstract)



[16]杜雄明, 孙君灵, 周忠丽, 贾银华, 潘兆娥, 何守朴, 庞保印, 王立如. 棉花种质资源收集、保存评价与利用现状及未来. 植物遗传资源学报, 2012, 13: 163–168



Du X M, Sun J L, ZhouZ L, Jia Y H, Pan Z E, He S P, Pang B Y, Wang L R. Current situation and the future in collection, preservation, evaluation and utilization of cotton germplasm in China. J Plant Genet Resour, 2012, 13: 163–168 (in Chinese with English abstract)



[17]河北省地方标准. 棉花耐盐性鉴定评价技术规范(DB13/T 1339-2010). 唐山:河北省质量技术监督局, 2011



The local standard for Hebei Province. Rules for characterization and evaluation of cotton salt tolerance (DB13/T1339-2010). Tangshan: Hebei Provincial Administration of Quality and Technical Supervision, 2011 (in Chinese).



[18]陈永坤, 汪宇. PEG模拟干旱胁迫对漾濞核桃幼苗抗性物质的影响. 西南林业大学学报, 2013, 33:103–106



Chen Y K, Wang Y. Effects of drought stress simulated by PEG on resistant substances of Juglans sigillata seedlings. J Southwest For Univ, 2013, 33:103–106 (in Chinese with English abstract)



[19]Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol, 1995, 61: 32–42 (in Chinese)



[20]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000



Li H S. Plant physiological and Biochemical Experiment Principle and Technology. Beijing: Higher Education Press, 2000 (in Chinese)



[21]李忠光, 李江鸿, 杜朝昆, 黄号栋, 龚明. 在单一提取系统中同时测定五种植物抗氧化酶. 云南师范大学学报, 2002, 22: 44–45



Li Z G, Li J H, Du C K, Huang H D, Gong M. Simultaneous measurement of five antioxidant enzyme activities using a single extraction system. J Yunan Norm Univ (Nat Sci Edn), 2002, 22: 44–45 (in Chinese with English abstract)



[22]钮福祥, 华希新, 郭小丁, 邬景禹, 李洪民, 丁成伟.甘薯品种抗旱性生理指标及其综合评价初探. 作物学报, 1996, 22: 293–295



Niu F X, Hua X X, Guo X D, Wu J Y, Li H M, Ding C W. Studies on several physiological indexes of the drought resistance of sweet potato and its comprehensive evaluation. Acta Agron Sin, 1996, 22: 293–295 (in Chinese with English abstract)



[23]孙效功, 杨作升. 基于灰色关联度的聚类分析方法. 青岛海洋大学学报, 1995, 25: 229–231



Sun X G, Yang Z S. Cluster analysis based on the gray relational grades. Period Ocean Univ China, 1995, 25: 229–231 (in Chinese with English abstract)



[24]吴凤祥. 多指标评价中指标权重的计算方法研究. 河北林业科技, 1992, 3: 44–46



Wu F X. Multi-index evaluation index weight calculation method. J Hebei For Sci Technol, 1992, 3: 44–46 (in Chinese)



[25]Ashraf M, Ahmad S. Influence of sodium chloride onion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Res, 2000, 66: 115–127



[26]张锦伟, 许键, 杨改刚, 谭学林. 用不同浓度NaCl溶液筛选水稻苗期耐盐抗旱材料. 西南农业学报, 2004, 17: 81–83



Zhang J W, Xu J, Yang G G, Tan X L. Screening of rice lines in salt and drought tolerance with NaCl solutions. Southwest China J Agricl Sci, 2004, 17:81–83(in Chinese with English abstract)



[27]解松峰, Kansaye A, 杜向红, 聂小军, 方桂英. 30份引进大麦品种(系)苗期耐盐性综合分析.草业科学, 2010, 27: 127–133



Xie S F, Aly K, Du X H, Nie X J, Fang G Y. Comprehensive analysis of salt tolerance of 30 introduced barley varieties or lines in seedling period. Pratac Sci, 2010, 27: 127–133 (in Chinese with English abstract)



[28]张国新, 王秀萍, 鲁雪林, 刘雅辉. 隶属函数法鉴定水稻品种耐盐性. 安徽农学通报, 2011, 17: 36–39



Zhang G X, Wang X P, Lu X L, Liu Y H. Identification of membership functions salt tolerance of rice varieties. Anhui Agric Sci Bull, 2011, 17: 36–39 (in Chinese)



[29]刘雅辉, 王秀萍, 张国新, 鲁雪林, 张亚丽.棉花苗期耐盐生理指标的筛选及综合评价. 中国农学通报, 2012, 28: 73–78



Liu Y H, Wang X P, Zhang G X, Lu X L, Zhang Y L. Study on selection of physiological indices for salt tolerance and comprehensive evaluation of cotton during seedling stage. Chin Agric Sci Bull, 2012, 28: 73–78 (in Chinese with English abstract)



[30]Ashraf M. Breeding for salinity tolerance in plants. Critical Rev Plant Sci, 1994, 13:17–42



[31]王俊娟, 王德龙, 樊伟莉, 宋贵方, 王帅, 叶武威. 陆地棉萌发至三叶期不同生育阶段耐盐特性. 生态学报, 2011, 31: 3720–3726



Wang J J, Wang D L, Fan W L, Song G F, Wang S, Ye W W. The characters of salt-tolerance at different growth stages in cotton. Acta Ecol Sin, 2011, 31: 3720–3727 (in Chinese with English abstract)



[32]Powle S B. Photo inhibition of photosynthesis induced by visible light. Rev Plant Physiol, 1984, 35: 15–44



[33]林世青, 许春辉, 张其德, 徐黎, 毛大璋, 匡廷云. 叶绿素荧光动力学在植物抗性生理学、生态学和农业现代化中的应用. 植物学通报, 1992, 9: 1–16



Lin S Q, Xu C H, Zhang Q D, Xu L, Mao D Z, Kuang T Y. Some application of chlorophyll fluorescence kinetics to plant stress physiologyphy toecology and agricultural modernization. Chin Bull Bot, 1992, 9: 1–16 (in Chinese with English abstract)



[34]Kalaji H M, Govindjee, Bosa K, Ko?cielniak J, ?uk-Go?aszewska K. Effects of salt stress on photosystem II efficiency and CO2 assimilation of two Syrian barley landraces. Environ Exp Bot, 2011, 73: 64–72



[35]王仁雷, 华春, 刘友良. 盐胁迫对水稻光合特性的影响. 南京农业大学学报, 2002, 25: 11–14



Wang R L, Hua C, Liu Y L. Effect of salt stress on photosynthetic characteristics in rice. J Nanjing Agric Univ, 2002, 25: 11–14 (in Chinese with English abstract)



[36]Everard J D, Gucci R, Kann S C, Flore J A, Loescher W. H. Gas exchange and carbon partitioning in the leaves of celery (Aptium gravealens L.) at various levels of root zone salinity. Plant Physiol, 1994, 106: 281–292



[37]张国伟, 张雷, 唐明星, 周玲玲, 陈兵林, 周治国. 土壤盐分对棉花功能叶气体交换参数和叶绿素荧光参数日变化的影响. 应用生态学报, 2011, 22: 1772–1773



Zhang G W, Zhang L, Tang M X, Zhou L L, Chen B L, Zhou Z G. Diurnal variation of gas exchange and chlorophyll fluorescence parameters of cotton functional leaves under effects of soil salinity. Chin J Appl Ecol, 2011, 22: 1772–1773 (in Chinese with English abstract)



[38]Li G, Wan S W, Zhou J, Yang Z Y, Qin P. Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinusco mmunis L.) seedlings to salt stress levels. Indust Crops Products, 2010, 31: 13–19

[1] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[2] Li-Ge BAO,Ping LU,Meng-Sha SHI,Yue XU,Min-Xuan LIU. Screening and identification of Chinese sorghum landraces for salt tolerance at germination and seedling stages [J]. Acta Agronomica Sinica, 2020, 46(5): 734-744.
[3] Mao-Ni CHAO,Hai-Yan HU,Run-Hao WANG,Yu CHEN,Li-Na FU,Qing-Qing LIU,Qing-Lian WANG. Cloning and functional analysis of promoter of potassium transporter gene GhHAK5 in upland cotton (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2020, 46(01): 40-51.
[4] ZHANG Chun-Xiao,LI Shu-Fang,JIN Feng-Xue,LIU Wen-Ping,LI Wan-Jun,LIU Jie,LI Xiao-Hui. QTL mapping of salt and alkaline tolerance-related traits at the germination and seedling stage in maize (Zea mays L.) using three analytical methods [J]. Acta Agronomica Sinica, 2019, 45(4): 508-521.
[5] Xiao-Hong ZHANG,Gen-Hai HU,Han-Tao WANG,Cong-Cong WANG,Heng-Ling WEI,Yuan-Zhi FU,Shu-Xun YU. Expression and promoter activity of GhTFL1a and GhTFL1c in Upland cotton [J]. Acta Agronomica Sinica, 2019, 45(3): 469-476.
[6] Mi WU,Nian WANG,Chao SHEN,Cong HUANG,Tian-Wang WEN,Zhong-Xu LIN. Development and evaluation of InDel markers in cotton based on whole-genome re-sequencing data [J]. Acta Agronomica Sinica, 2019, 45(2): 196-203.
[7] SUN Xian-Jun,JIANG Qi-Yan,HU Zheng,ZHANG Hui-Yuan,XU Chang-Bing,DI Yi-Huan,HAN Long-Zhi,ZHANG Hui. Screening and identification of salt-tolerant rice germplasm in whole growth period [J]. Acta Agronomica Sinica, 2019, 45(11): 1656-1663.
[8] ZHANG Xiao-Xiao,PAN Ying-Hong,REN Fu-Li,PU Wei-Jun,WANG Dao-Ping,LI Yu-Bin,LU Ping,LI Gui-Ying,ZHU Li. Establishment of an accurate evaluation method for drought resistance based on multilevel phenotype analysis in sorghum [J]. Acta Agronomica Sinica, 2019, 45(11): 1735-1745.
[9] Cong HUANG,Xiao-Fang LI,Ding-Guo LI,Zhong-Xu LIN. QTL Mapping for Yield, Growth Period and Plant Height Traits Using MAGIC Population in Upland Cotton [J]. Acta Agronomica Sinica, 2018, 44(9): 1320-1333.
[10] Wen-Xue DUAN,Hai-Yan ZHANG,Bei-Tao XIE,Bao-Qing WANG,Li-Ming ZHANG. Identification of Salt Tolerance and Screening for Its Indicators in Sweet Potato Varieties during Seedling Stage [J]. Acta Agronomica Sinica, 2018, 44(8): 1237-1247.
[11] Chao LI,Zhi-Kun LI,Qi-Shen GU,Jun YANG,Hui-Feng KE,Li-Qiang WU,Guo-Ning WANG,Yan ZHANG,Jin-Hua WU,Gui-Yin ZHANG,Yuan-Yuan YAN,Zhi-Ying MA,Xing-Fen WANG. Molecular Evaluation for Chromosome Segment Substitution Lines of Gossypium barbadense and QTL Mapping for Fiber Quality and Yield [J]. Acta Agronomica Sinica, 2018, 44(8): 1114-1126.
[12] Long LI,Xin-Guo MAO,Jing-Yi WANG,Xiao-Ping CHANG,Yu-Ping LIU,Rui-Lian JING. Drought Tolerance Evaluation of Wheat Germplasm Resources [J]. Acta Agronomica Sinica, 2018, 44(7): 988-999.
[13] Guo-Zhong ZHU,Fang ZHANG,Jie FU,Le-Chen LI,Er-Li NIU,Wang-Zhen GUO. Genome-wide Screening and Evaluation of SNP Core Loci for Identification of Upland Cotton Varieties [J]. Acta Agronomica Sinica, 2018, 44(11): 1631-1639.
[14] Dong-Liang CHEN, Cui CUI, Yi-Ying REN, Qian WANG, Jia-Na LI, Zhang-Lin TANG, Qing-Yuan ZHOU. Genome-wide Association Analysis of Some Phytotoxicity Related Traits at Seedling Stage in Rapeseed under Glufosinate Stress [J]. Acta Agronomica Sinica, 2018, 44(04): 542-553.
[15] Mao-Ni CHAO, Qing-Yu WEN, Zhi-Yong ZHANG, Gen-Hai HU, Jin-Bao ZHANG, Guo WANG, Qing-Lian WANG. Sequence Characteristics and Expression Analysis of Potassium Transporter Gene GhHAK5 in Upland Cotton (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2018, 44(02): 236-244.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!