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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 658-665.doi: 10.3724/SP.J.1006.2015.00658

• RESEARCH NOTES • Previous Articles     Next Articles

Establishment of 20 Sorghum Hybrids Fingerprints Using SSR Fluorescent Marker

WANG Rui1,ZHANG Fu-Yao1,*,CHENG Qing-Jun1,TIAN Cheng-Hua1,LING Liang2   

  1. 1Sorghum Institute, Shanxi Academy of Agricultural Sciences / Key Laboratory of Genetic and Germplasm Innovation in sorghum for Shanxi Province, Shanxi 030600, China; 2Institute of Edible Fungi, Shanxi Academy of Agricultural Sciences, Shanxi 030031, China
  • Received:2014-10-08 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-02
  • Contact: 张福耀, E-mail: zfy5607@163.com, Tel: 13835425607 E-mail:wangrui989@163.com

Abstract:

To establish a standard technical system for purity and authenticity identification of sorghum seeds,it is necessary to construct a DNA fingerprinting database of major sorghum hybrids. The fluorescent marker detection of simple sequence repeat (SSR) was used to establish SSR fingerprints of 20 sorghum hybrids that are popularized in medium and late-maturing areas in China since heterosis has been utilized. Thirty-six pairs of SSR primers were screened and 235 alleles were amplified with the set of primers. The number of alleles per locus ranged from two to twelve with an average of seven. The range of gene diversity and polymorphism information content (PIC) at 36 SSR loci of 20 hybrids were 0.3490–0.8813 and 0.3144–0.8696, with an average of 0.6976 and 0.6571, respectively. Among 36 SSR primers, two primer pairs with high polymorphism were identified as the specific primers for distinguishing the all 20 sorghum hybrids, resulting in the fingerprints for the 20 sorghum hybrids. The presented data and technology provides an important basis for hybrids identification in sorghum.

Key words: Sorghum, Hybrid identification, SSR fluorescent marker, Fingerprint

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