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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 666-670.doi: 10.3724/SP.J.1006.2015.00666

• RESEARCH NOTES • Previous Articles    

Secretion of Citrate from Root Apices and Expression of SGA1 in Soybean under AlCl3 Stress

YANG Lie-Geng2,**,YANG Shu2,**,ZHANG Yong-Xian2,TANG Jian1,LI Xiao-Feng2,*   

  1. 1 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation / Guangxi Forestry Research Institute, Nanning 530002, China; 2 Guangxi University / State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources, Nanning 530004, China
  • Received:2014-11-02 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-03
  • Contact: 黎晓峰, E-mail: lxf@gxu.edu.cn, Tel: 0771-3235314 E-mail:74123810@qq.com

Abstract:

The effects of Al3+ on the secretion of organic acids from root apices and the expression of SGAI gene were investigated by hydroponics to elucidate the characteritics of organic acid secretion and Al3+ stress signal transduction pathway which mediates the secretion of organic acids in soybean Guangzhou 2. The results showed that soybean root apices (in vivo) secreted citrate under Al3+ stress. The secretion of citrate increased with the increase of Al3+ concentrations (25, 50 µmol L–1 AlCl3) and the prolongation (2–12 hours) of treatment with Al3+. Citrate was secreted from root apices by pattern II in soybean. The secretion rate was very low within initial four hours after Al3+ treatment but remarkably elevated thereafter. A gap of time between the secretion and Al3+ treatment reached to about six hours. On the other hand when cholera toxin, an inhibitor of heterotrimeric G-protein, was added to Al3+solution, the amount of citrate secreted decreased by 38.7%. RT-PCR analysis results indicated that Al3+ induced SGA1 expression. In general, the expression level was elevated with the prolongation of treatment with Al3+ (0.5 to 12 hours). Moreover, Al3+ induced expression of SGA1 sooner than the secretion of citrate. These results imply that Al3+ induces  the secretion of citrate from root apices and SGA1 expression in the soybean, and heterotrimeric G proteins may act as a switch of Al3+ stress signal to be involved in the regulation of citrate secretion from root apices under Al3+ stress.

Key words: AlCl3, Soybean, Citrate, Heterotrimeric G-protein, SGA1 gene

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