Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 666-670.doi: 10.3724/SP.J.1006.2015.00666

• RESEARCH NOTES • Previous Articles    

Secretion of Citrate from Root Apices and Expression of SGA1 in Soybean under AlCl3 Stress

YANG Lie-Geng2,**,YANG Shu2,**,ZHANG Yong-Xian2,TANG Jian1,LI Xiao-Feng2,*   

  1. 1 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation / Guangxi Forestry Research Institute, Nanning 530002, China; 2 Guangxi University / State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources, Nanning 530004, China
  • Received:2014-11-02 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-03
  • Contact: 黎晓峰, E-mail: lxf@gxu.edu.cn, Tel: 0771-3235314 E-mail:74123810@qq.com

Abstract:

The effects of Al3+ on the secretion of organic acids from root apices and the expression of SGAI gene were investigated by hydroponics to elucidate the characteritics of organic acid secretion and Al3+ stress signal transduction pathway which mediates the secretion of organic acids in soybean Guangzhou 2. The results showed that soybean root apices (in vivo) secreted citrate under Al3+ stress. The secretion of citrate increased with the increase of Al3+ concentrations (25, 50 µmol L–1 AlCl3) and the prolongation (2–12 hours) of treatment with Al3+. Citrate was secreted from root apices by pattern II in soybean. The secretion rate was very low within initial four hours after Al3+ treatment but remarkably elevated thereafter. A gap of time between the secretion and Al3+ treatment reached to about six hours. On the other hand when cholera toxin, an inhibitor of heterotrimeric G-protein, was added to Al3+solution, the amount of citrate secreted decreased by 38.7%. RT-PCR analysis results indicated that Al3+ induced SGA1 expression. In general, the expression level was elevated with the prolongation of treatment with Al3+ (0.5 to 12 hours). Moreover, Al3+ induced expression of SGA1 sooner than the secretion of citrate. These results imply that Al3+ induces  the secretion of citrate from root apices and SGA1 expression in the soybean, and heterotrimeric G proteins may act as a switch of Al3+ stress signal to be involved in the regulation of citrate secretion from root apices under Al3+ stress.

Key words: AlCl3, Soybean, Citrate, Heterotrimeric G-protein, SGA1 gene

[1]Mac D T L, Humphreys W G, Martin R B. Promotion of tubulin assembly by aluminum ion in vitro. Science, 1987, 236: 183–186



[2]Kochian L V. Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Physiol Plant Mol Biol, 1995, 46: 237–260



[3]Taylor G J. Current views of the aluminum stress response: the physiological basis of tolerance. Curr Top Plant Biochem Physiol, 1991, 10: 57–93



[4]Ma J F. Role of organic acids in detoxification of aluminum in higher plants. Plant Cell Physiol, 2000, 41: 383–390



[5]Chen Y L, Huang R F, Xiao Y M, Lu P, Chen J, Wang X C. Extracellular calmodulin-induced stomatal closure is mediated by heterotrimeric G protein and H2O2. Plant Physiol, 2004, 136: 4096–4103



[6]Assmann S M. Heterotrimeric and unconventional GTP binding proteins in plant cell signaling. Plant Cell, 2002, 14: S355–S373



[7]Fujisawa Y, Kao H, Iwasaki Y. Structure and function of heterotfimeric G proteins in plants. Plant Cell Physiol, 2001, 42, 789–794



[8]Urano D, Jones A M. Heterotrimeric G protein-coupled signaling in plants. Annu Rev Plant Biol, 2014, 65: 365–84



[9]Yang Z M, Sivaguru M, Horst W J, Matsumoto H. Aluminium tolerance is achieved by exudation of citric acid from roots of soybean (Glycine max). Physiol Plant, 2000, 110: 72–77



[10]Li Y Y, Tang X L, Yang L G, Yu Y X, Li X F. Possible involvement of heterotrimeric G-protein signaling in Al-induced secretion of organic acid anions in Arabidopsis and rye. Plant Soil, 2014, DOI: 10.1007/s11104-014-2325-0



[11]Li X F, Zuo F H, Ling G Z, Li Y Y, Yu Y Y, Yang P Q, Tang X L. Secretion of citrate from roots in response to aluminum and low phosphorus stresses in Stylosanthes. Plant Soil, 2009, 325: 219–229



[12]Ryan P R, Ditomaso J M, Kochian L V. Aluminum toxicity in roots: an investigation of spatial sensitivity and to role of the root cap. J Exp Bot, 1993, 44: 43–446



[13]Zheng S J, Ma J F, Matusmoto H. High aluminum resistance in buckwheat. I: Al-induced specific secretion of oxalic acid from root tips. Plant Physiol, 1998, 117: 745–751



[14]Dong D F, Peng X X, Yan X L. Organic acid exudation induced by phosphorus deficiency and/or aluminum toxicity in two contrasting soybean genotypes. Physiol Plant, 2004, 122: 190–199



[15]Li X F, Ma J F, Matusmoto H. Pattern of Al induced secretion of organic acid differs between rye and wheat. Plant Physiol, 2000, 123: 1537–1543



[16]Ma H. GTP-binding proteins in plants: new members of an old family. Plant Mol Biol, 1994, 26: 1611–1636



[17]Liu J P, Magalhaes J V, Shaff J, Kochian L V. Aluminum activated citrate and malate transporters from the MATE and ALMT families function independently to confer Arabidopsis aluminum tolerance. Plant J, 2009, 57: 389–399



[18]Delhaize E, Ma J F, Ryan P R. Transcriptional regulation of aluminium tolerance genes. Trends Plant Sci, 2012, 17: 341–348

[1] Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling. Genome-wide association analysis and prediction model construction for soybean plant height [J]. Acta Agronomica Sinica, 2026, 52(6): 1743-1756.
[2] Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[3] Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[4] WANG Ke-Jing, LI Xiang-Hua. Endangerment assessment of the perennial species G. tabacina and G. tomentella of the genus Glycine Willd. in China [J]. Acta Agronomica Sinica, 2025, 51(8): 2009-2019.
[5] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[6] HE Hong-Li, ZHANG Yu-Han, YANG Jing, CHENG Yun-Qing, ZHAO Yang, LI Xing-Nuo, SI Hong-Liang, ZHANG Xing-Zheng, YANG Xiang-Dong. Creation and physiological analysis of an e1-as gene mutant in soybean [J]. Acta Agronomica Sinica, 2025, 51(8): 2228-2239.
[7] HU Meng, SHA Dan, ZHANG Sheng-Rui, GU Yong-Zhe, ZHANG Shi-Bi, LI Jing, SUN Jun-Ming, QIU Li-Juan, LI Bin. QTL mapping and candidate gene screening for branch number in soybean [J]. Acta Agronomica Sinica, 2025, 51(7): 1747-1756.
[8] WANG Qiong, ZOU Dan-Xia, CHEN Xing-Yun, ZHANG Wei, ZHANG Hong-Mei, LIU Xiao-Qing, JIA Qian-Ru, WEI Li-Bin, CUI Xiao-Yan, CHEN Xin, WANG Xue-Jun, CHEN Hua-Tao. Genome-wide association analysis and candidate genes prediction of flowering time and maturity date traits in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2025, 51(6): 1558-1568.
[9] YIN Cong-Cong, LI Rui-Qi, YUE Pei-Yao, LI Chen, NIU Jing-Ping, ZHAO Jin-Zhong, DU Wei-Jun, YUE Ai-Qin. Establishment and application of a visual detection method for soybean mosaic virus SC15 based on closed dumbbell mediated isothermal amplification [J]. Acta Agronomica Sinica, 2025, 51(5): 1248-1260.
[10] XU Rui, HE Miao-Hua, WANG Hao, LI Wei, REN Jie, XIA Zhi-Qiang. Spatial transcriptomic analysis of soybean embryonic responses to X-ray irradiation [J]. Acta Agronomica Sinica, 2025, 51(12): 3121-3132.
[11] LIN Yang, SHI Xiao-Lei, CHEN Qiang, LIU Bing-Qiang, YANG Qing, YU Hui-Juan, YAN Long, WU Xiao-Xia, YANG Chun-Yan. QTL mapping of soybean protein, oil, and fatty acid components [J]. Acta Agronomica Sinica, 2025, 51(11): 2899-2910.
[12] LI Wei, ZHU Yu-Peng, SUN Bin-Cheng, WEN You-Xiang, WU Zong-Sheng, XU Yi-Fan, SONG Wen-Wen, XU Cai-Long, WU Cun-Xiang. Transgenic soybean combined with no-tillage flat planting promotes the simplification of soybean production in Northeast China [J]. Acta Agronomica Sinica, 2025, 51(10): 2738-2749.
[13] CHEN Min, JIA Rong, ZHANG Jin-Chuan, ZHANG Chen-Yu, CHU Jun-Cong, YAO Wei, GE Jun-Yong, WANG Xing-Yu, YANG Ya-Dong, ZENG Zhao-Hai, ZANG Hua-Dong. Yield advantages and nitrogen utilization characteristics of oat and legume strip intercropping in semi-arid zones [J]. Acta Agronomica Sinica, 2025, 51(10): 2727-2737.
[14] QIAN Yu-Ping, SU Bing-Bing, GAO Ji-Xing, RUAN Fen-Hua, LI Ya-Wei, MAO Lin-Chun. Effects of maize and soybean intercropping on soil physicochemical properties and microbial carbon metabolism in karst region [J]. Acta Agronomica Sinica, 2025, 51(1): 273-284.
[15] DING Shu-Qi, CHENG Tong, WANG Bi-Kun, YU De-Bin, RAO De-Min, MENG Fan-Gang, ZHAO Yin-Kai, WANG Xiao-Hui, ZHANG Wei. Effects of planting density on photosynthetic production and yield formation of soybean varieties from different eras [J]. Acta Agronomica Sinica, 2025, 51(1): 161-173.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!