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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (11): 1657-1662.doi: 10.3724/SP.J.1006.2015.01657


Sequence and Pathogenicity of Recombined Soybean Mosaic Virus Isolate from Hebei Province, China

LIN Jing1,2,YANG Yong-Qing2,3,HOU Wen-Huan2,YANG Chun-Yan2,XIE Ling-Qin1,*,ZHI Hai-Jian3,ZHANG Meng-Chen2,*   

  1. 1 College of Agronomy, Agricultural University of Hebei, Baoding, 071001; 2 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / National Soybean Improvement Center Shijiazhuang Sub-Center / Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture / Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, China; 3.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing 210095, China
  • Received:2015-04-04 Revised:2015-07-10 Online:2015-11-12 Published:2015-08-12


Recombined Soybean mosaic virus (SMV-R), which is prevalent in many soybean production regions, is a novel type of SMV. To clarify the characteristics of recombinant SMV structure and pathogenicity of SMV-R, we sequenced the whole genomes of a SMV-R isolate (HB-RS) from Hebei province of China, and compered the difference of SMV infection and accumulation in four soybean cultivars with SMV-R. The results showed that besides the poly-A tail, HB-RS consists of 9993 nucleotides, encoding only one open reading frame and 3202 amino acids. Phylogenetic analysis showed that the HB-RS isolate was clustered with other two recombined SMV isolates. Resistance identification results showed that the average disease index of four cultivars resistant to HB-RS and Sc6 was 59.5 and 60.5, respectively. The same soybean cultivar had different symptoms and resistance levels to different strains (isolates), Jidou 17 showed high resistance to Sc6 and moderate resistance to HB-RS. These results indicated that soybean resistance to SMV exists strain (isolate) specialization. Additionally, the pathogenicity test showed that accumulation of HB-RS in four cultivars was higher than that of Sc6, indicating that HB-RS is more adaptable to the host plants. The host with highest accumulation of HB-RS was Nannong 1138-2, with 522-fold of reference virus accumulation, and the following was Wuxing 1 (471 U), Jihuang 13 (199 U) and, Jidou 17 (only 90 U), suggesting that HB-RS has a more survival adaptability in the soybean host. However different resistance levels to HB-RS were observed in various soybean cultivars and Jidou 17 could be used as a resistant cultivar in production or parents in further breeding.

Key words: Recombined SMV, Genome, Virus titer, Pathogenicity

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