[1] Cai X Z, Xu Q F, Wang C C, Zheng Z. Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis. Plant Mol Biol, 2006, 62: 223–232
[2] Hou P Q, Lee Y I, Hsu K T, Lin Y T, Wu W Z, Lin J Y, Nam T N, Fu S F. Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing. Physiol Plant, 2014, 150: 119–132
[3] 黄赛花, 郑桂杰, 杨永庆,智海剑. 利用VIGS技术对抗SMV候选基因GmZ15的功能分析. 大豆科学, 2015, 34: 582–587
Huang S H, Zheng G J, Yang Y Q, Zhi H J. Analysis on the candidate resistance gene GmZ15 to soybean mosaic virus by VIGS. Soybean Sci, 2015, 34: 582–587 (in Chinese with English abstract)
[4] Becker A, Lange M. VIGS–genomics goes functional. Trends Plant Sci , 2010, 15: 1–4
[5] Ratcliff F G, MacFarlane S A, Baulcombe D C. Gene silencing without DNA: RNA-mediated cross protection between viruses. Plant Cell, 1999, 11: 1207–1215
[6] Gao X Q, Wheeler T, Li Z H, Kenerley C M, He P, Shan L B. Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt. Plant J, 2011, 66: 293–305
[7] 王丽, 穆春, 张明才, 杜明伟, 田晓莉, 李召虎．GhCPS基因沉默对棉花幼苗生长和内源激素含量的影响. 棉花学报, 2014, 26: 189–196
Wang L, Mu C, Zhang M C, Du M W, Tian X L, Li Z H. Effect of silencing GhCPS on the growth and endogenous hormone content of cotton seedlings(Gossypium himutum L.) Cotton Sci, 2014, 26: 189–196 (in Chinese with English abstract)
[8] 王心宇, 吕坤, 蔡彩平, 徐君, 郭旺珍. TRV病毒介导的基因沉默体系在棉花中的建立及应用. 作物学报, 2014, 40: 1356–1363
Wang X Y, Lü K, Cai C P, Xu J, Guo W Z. Establishment and application of TRV-mediated virus-induced gene silencing in cotton. Acta Agron Sin, 2014, 40: 1356–1363 (in Chinese with English abstract)
[9] Faivre-Rampant O, Gilroy E M, Hrubikova K, Hein I, Millam S, Loake G J, Birch P, Taylor M, Lacomme C. Potato virus X-induced gene silencing in leaves and tubers of potato. Plant Physiol, 2004, 134:1308–1316
[10] Liu E, Page J E. Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus. Plant Methods, 2008, 4: 1–13
[11] Lichtenthaler H K, Wellbum A R. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans, 1983, 11: 591–592
[12] Fu D Q, Zhu B Z, Zhu H L, Zhang H X, Xie Y H, Jiang W B, Zhao X D, Luo Y B. Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol. Cells, 2006, 21: 153–160
[13] Tuttle J R, Idris A M, Brown J K, Haigler C H, Robertson D. Geminivirus-mediated gene silencing from cotton leaf crumple virus is enhanced by low temperature in cotton. Plant Physiol. 2008, 148: 41–50
[14] Szittya G, Silhavy D, Molnar A, Havelda Z, Lovas A, Lakatos L, Banfalvi Z, Burgyan J. Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. Embo J, 2003, 22: 633–640
[15] Burch‐Smith T M, Anderson J C, Martin G B, Dinesh‐Kumar S P. Applications and advantages of virus‐induced gene silencing for gene function studies in plants. Plant J , 2004, 39: 734–746
[16] Nethra P, Nataraja K N, Rama N, Udayakumar M. Standardization of environmental conditions for induction and retention of post-transcriptional gene silencing using tobacco rattle virus vector. Curr Sci, 2006, 90: 431–435
[17] Ekengren S K, Liu Y L, Schiff M, Dinesh-Kumar S P, Martin G B, Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato. Plant J, 2003, 36: 905–917
[18] Hartl M, Merker H, Schmidt D D, Baldwin I T. Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls. New Phytol, 2008, 179: 356–365
[19] Wang C C, Cai X Z, Wang X M, Zheng Z. Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis. Funct Plant Biol, 2006, 33: 347–355
[20] Kieber J J, Rothenberg M, Roman G, Feldmann K A, Ecker J R. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raffamily of protein kinases. Cell, 1993, 72: 427–441