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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1577-1591.doi: 10.3724/SP.J.1006.2016.01577

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Genome-wide Identification of Acyl-CoA-Binding Protein (ACBP) Gene Family and Their Functional Analysis in Abiotic Stress Tolerance in Cotton

QIN Peng-Fei,SHANG Xiao-Guang,SONG Jian,GUO Wang-Zhen*   

  1. State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2016-02-29 Revised:2016-07-11 Online:2016-11-12 Published:2016-08-11
  • Contact: Guo Wangzhen, E-mail: moelab@njau.edu.cn, Tel: 025-84396523
  • Supported by:

    This program was financially supported in part by the National Transgenic Program (2011ZX08005-004) and Jiangsu Collaborative Innovation Center for Modern Crop Production project (No.10).

Abstract:

Acyl-CoA-binding protein (ACBP) plays important roles in plant development, including normal growth, response to biotic/abiotic stress and membrane system repairing. However, the functions of ACBPs in cotton still remain largely unknown. Based on the G. hirsutum TM-1 genome database, we identified 21 ACBP genes and obtained their sequence information, chromosomal location. Phylogenetic analysis revealed that the 21 ACBP genes were classified into four groups. According to the nomenclature of A. thaliana homologous gene, the cotton ACBP genes were named as GhACBP1–GhACBP6 subclasses. Transcriptome data showed that the expression patterns of ACBP genes varied significantly in different cotton tissues. Further abiotic stresses treatment analysis showed that GhACBP1, GhACBP3, and GhACBP6 sub-class genes could be significantly induced by drought, salt, low temperature and high temperature treatments, while GhACBP4 and GhACBP5 sub-class genes could not. GhACBP3 and GhACBP6 could also be significantly induced by hydrogen peroxide (H2O2), salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), and ethylene (ET). Virus induced gene silencing (VIGS) results exhibited that down-regulation of GhACBP3 and GhACBP6 sub-class genes would significantly reduce the plant tolerance to drought and salt stresses. Compared with the control, the GhACBP3 and GhACBP6 silencing plants demonstrated significantly reduced plant dry matter, decreased plant height, lower superoxide dismutase (SOD) and peroxidase (POD) activities, and

Key words: Cotton, ACBP gene family, Structure, Expression, Drought stress, Salt stress

[1]. Liu F, Zhang X, Lu C, Zeng X, Li Y, Fu D, Wu G. Non-specific lipid transfer proteins in plants: presenting new advances and an integrated functional analysis. J Exp Bot, 2015, 66: 5663–5681 [2]. Guidotti A, Forchetti C M, Corda M G, Konkel D, Bennett C D, Costa E. Isolation, characterization, and purification to homogeneity of an endogenous polypeptide with agonistic action on benzodiazepine receptors. Proc Natl Acad Sci USA, 1983, 80: 3531–3535 [3]. Alho H, Costa E, Ferrero P, Fujimoto M, Cosenza-Murphy D, Guidotti A. Diazepam-binding inhibitor: a neuropeptide located in selected neuronal populations of rat brain. Science, 1985, 229: 179–182 [4]. Weselake R J, Nykiforuk C L, Laroche A, Patterson N A, Wiehler W B, Szarka S J, Moloney M M, Tari L W, Derekh U. Expression and properties of diacylglycerol acyltransferase from cell-suspension cultures of oilseed rape. Biochem Soc T, 2000, 28. 6 [5]. Hills M J, Dann R, Lydiate D, Sharpe A. Molecular cloning of a cDNA from Brassicanapus L. for a homologue of acyl-CoA-binding protein. Plant Mol Biol, 1994, 25: 917–920 [6]. Engeseth N J, Pacovsky R S, Newman T, Ohlrogge J B. Characterization of an Acyl-CoA-Binding Protein from Arabidopsis thaliana. Arch Biochem Biophys, 1996, 331: 55–62 [7]. Erber A, Horstmann C, Schobert C. A cDNA clone for acyl-CoA-binding protein from castor bean. Plant Physiol, 1997, 114: 396–396 [8]. Metzner M L, Ruecknagel K P, Knudsen J, Kuellertz G, Mueller-Uri F, Diettrich B. Isolation and characterization of two acyl-CoA-binding proteins from proembryogenic masses of Digitalis lanataEhrh. Planta, 2000, 210: 683–685 [9]. Guerrero C, Martín-Rufián M, Reina JJ, Heredia A. Isolation and characterization of a cDNA encoding a membrane bound acyl-CoA binding protein from Agave americana L. epidermis. Plant Physiol Bioch, 2006, 44: 85–90 [10]. Reddy A, Ranganathan B, Haisler R, Swize M. A cDNA encoding acyl-CoA-binding protein from cotton. Plant Physiol, 1996, 111: 348 [11]. Burton M, Rose T M, Faergeman N J, Knudsen J. Evolution of the acyl-CoA binding protein (ACBP). Biochem J, 2005, 392: 299–307 [12]. Xiao S, Chye M L. New roles for acyl-CoA-binding proteins (ACBPs) in plant development, stress responses and lipid metabolism. Prog Lipid Res, 2011, 50: 141–151 [13]. Fan J, Liu J, Culty M, Papadopoulos V. Acyl-coenzyme A binding domain containing 3 (ACBD3; PAP7; GCP60): an emerging signaling molecule. Prog Lipid Res, 2010, 49: 218–234 [14]. Xiao S, Chye M L. An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members. Plant Physiol Bioch, 2009, 47: 479–484 [15]. Kannan L, Knudsen J, Jolly C A. Aging and acyl-CoA binding protein alter mitochondrial glycerol-3-phosphate acyltransferase activity. Biochim Biophys Acta, 2003, 1631: 12–16 [16]. Knudsen J, Burton M, Faergeman N. Long chain acyl-CoA esters and acyl-CoA binding protein (ACBP) in cell function. Advances in Molecular and Cell Biology: Elsevier, 2003. pp: 123–152 [17]. Faergeman N J, Feddersen S, Christiansen J K, Larsen M K, Schneiter R, Ungermann C, Mutenda K, Roepstorff P, Knudsen J. Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae. Biochem J, 2004, 380: 907–918 [18]. Gaigg B, Neergaard T B, Schneiter R, Hansen J K, Faergeman N J, Jensen N A, Andersen J R, Friis J, Sandhoff R, Schr?der H D, Knudsen J. Depletion of Acyl-Coenzyme A-Binding Protein Affects Sphingolipid Synthesis and Causes Vesicle Accumulation and Membrane Defects in Saccharomyces cerevisiae. Mol Biol Cell, 2001, 12: 1147–1160 [19]. Larsen M K, Tuck S, Faergeman N J, Knudsen J. MAA-1, a novel acyl-CoA-binding protein involved in endosomal vesicle transport in Caenorhabditis elegans. Mol Biol Cell, 2006, 17: 4318–4329 [20]. Chen Q F, Xiao S, Qi W, Mishra G, Ma J, Wang M, Chye M L. The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal. New Phytol, 2010, 186: 843–855 [21]. Baud S, Guyon V, Kronenberger J, Wuillème S, Miquel M, Caboche M, Lepiniec L, Rochat C. Multifunctional acetyl-CoA carboxylase 1 is essential for very long chain fatty acid elongation and embryo development in Arabidopsis. Plant J, 2003, 33: 75–86 [22]. Sellwood C, Slabas A, Rawsthorne S. Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos. Biochem Soc T, 2000, 28: 598–600 [23]. Gao W, Xiao S, Li H Y, Tsao S W, Chye M L. Arabidopsis thaliana acyl-CoA-binding protein ACBP2 interacts with heavy-metal-binding farnesylated protein AtFP6. New Phytol, 2009, 181: 89–102 [24]. Du Z Y, Chen M X, Chen Q F, Xiao S, Chye M L. Overexpression of Arabidopsis acyl-CoA-binding protein ACBP2 enhances drought tolerance. Plant Cell Environ, 2013, 36: 300-314. [25]. Du Z Y, Xiao S, Chen Q F, Chye M L. Depletion of the membrane-associated acyl-coenzyme A-binding protein ACBP1 enhances the ability of cold acclimation in Arabidopsis. Plant Physiol, 2010, 152: 1585–1597 [26]. Chen Q F, Xiao S, Chye M L. Overexpression of the Arabidopsis 10-kilodalton acyl-coenzyme A-binding protein ACBP6 enhances freezing tolerance. Plant Physiol, 2008, 148: 304–315 [27]. Meng W, Su Y C, Saunders R M, Chye M L. The rice acyl-CoA-binding protein gene family: phylogeny, expression and functional analysis. New Phytol, 2011, 189: 1170–1184 [28].戴海芳, 武辉, 阿曼古丽?买买提阿力, 王立红, 麦麦提?阿皮孜, 张巨松. 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选. 中国农业科学, 2014, 47: 1290–1300 Dai H F, Wu H, Amanguli?Maimaitiali, Wang L H, Maimaiti?Apizi, Zhang J S. Scientia Agricultura Sinica, 2014, 47: 1290–1300 (in Chinese with English abstract). [29]. Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J, Zhang J, Saski C A, Scheffler B E, Stelly D M, Hulse-Kemp A M, Wan Q, Liu B, Liu C, Wang S, Pan M, Wang Y, Wang D, Ye W, Chang L, Zhang W, Song Q, Kirkbride R C, Chen X, Dennis E, Llewellyn D J, Peterson D G, Thaxton P, Jones D C, Wang Q, Xu X, Zhang H, Wu H, Zhou L, Mei G, Chen S, Tian Y, Xiang D, Li X, Ding J, Zuo Q, Tao L, Liu Y, Li J, Lin Y, Hui Y, Cao Z, Cai C, Zhu X, Jiang Z, Zhou B, Guo W, Li R, Chen Z J. Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol, 2015, 33: 531–537 [30]. Zhang F, Li S, Yang S, Wang L, Guo W. Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton. Plant Mol Biol, 2015, 87: 47–67 [31]. Julie D. Thompson T J G, Frédéric P, Fran?ois J D G. Higgins. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876–4882 [32]. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011, 28: 2731–2739 [33]. Xiao S, Chye M L. Overexpression of Arabidopsis ACBP3 enhances NPR1-dependent plant resistance to Pseudomonas syringe pv tomato DC3000. Plant Physiol, 2011, 156: 2069–2081 [34]. Xiao S, Gao W, Chen Q F, Chan S W, Zheng S X, Ma J, Wang M, Welti R, Chye M L. Overexpression of Arabidopsis Acyl-CoA Binding Protein ACBP3 Promotes Starvation-Induced and Age-Dependent Leaf Senescence. Plant Cell, 2010, 22: 1463–1482 [35]. Chu X, Wang C, Chen X, Lu W, Li H, Wang X, Hao L, Guo X. The Cotton WRKY Gene GhWRKY41 Positively Regulates Salt and Drought Stress Tolerance in Transgenic Nicotiana benthamiana. PLoS One, 2015, 10: e0143022 [36]. Shi J, Zhang L, An H, Wu C, Guo X. GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity. BMC Mol Biol, 2011, 12: 22

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