Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1609-1619.doi: 10.3724/SP.J.1006.2016.01609

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Construction and Genetic Evaluation of Chromosome Segment Substitution Linesin Tobacco(NicotianatabacumL.)

TONG Zhi-Jun, JIAO Fang-Chan, FANG Dun-Huang, CHEN Xue-Jun, WU Xing-Fu, ZENG Jian-Min, XIE He, ZHANG Yi-Han, and XIAO Bing-Guang*   

  1. Yunnan Academy of Tobacco Agricultural Sciences / Key Laboratory of Tobacco Biotechnological Breeding / National Tobacco Genetic Engineering Research Center, Kunming 650021, China
  • Received:2016-03-09 Revised:2016-07-11 Online:2016-11-12 Published:2016-07-28
  • Contact: 肖炳光,E-mail:xiaobg@263.net E-mail:tzj861@163.com
  • Supported by:

    ThisstudywassupportedbyChinaNationalTobaccoCompany(2014TBB01and110201301006(JY-06))andYunnanTobaccoCompany(2014YN18and2013YN01).

Abstract:

A set of chromosome segment substitution lines (CSSLs) of tobacco (Nicotiana tabacum L.) were first developed by molecular marker assisted selection (MAS) and successive backcrossing with Y3, the flue-cured tobacco germplasm with comprehensive traits as the recipient parent, and two common tobacco cultivars Beinhart1000-1 and K326 as the donor parents. The cigar tobacco cultivar Beinhart1000-1 carries a variety of resistance traits including black shank (race 0 and race 1) and brown spot resistance, while the flue-cured tobacco K326 is a commercial cultivar with high quality. In 256 CSSLs, a total of 377 substituted segments derived from donor Beinhart1000-1 and K326 with genetic background of Y3 distributed on 24 linkage groups. Each CSSL contained only 1–5 substituted segments and the length of substituted segments ranged from 0.05 to 36.88 cM with an average of 7.75 cM. The total length of the overlapped substituted segments was 2922.57 cM, which was 2.61 times of the whole tobacco genome length. And the CSSLs covered length was 1114.32 cM, with a covered ratio of 99.45% in the recurrent tobacco genome. The CSSLs constructed in this study are excellent genetic materials for gene mapping, QTL analysis of complicated traits, and developing varieties by marker assisted selection in Nicotiana tabacum L.

Key words: Tobacco(Nicotianatabacum L.), Chromosomesegmentsubstitutionlines(CSSLs), SSR, Molecularmarkerassistedselection(MAS)

[1]王元英,周健.中美主要烟草品种亲源分析与烟草育种.中国烟草学报,1995,13(5):11–22 WangYY,ZhouJ.GeneticanalysisofthemaintobaccocultivarsandthetobaccobreedinginUSAandChina.ActaTabSin,1995,13(5):11–22(inChinesewithEnglishabstract) [2]TongZJ,JiaoTL,WangFQ,LiMY,LenXD,GaoYL,LiYP,XiaoBG,WuWR.Mappingofquantitativetraitlociconferringresistancetobrownspotinflue-curedtobacco(NicotianatabacumL.).PlantBreed,2012,131:335–339 [3]TongZJ,JiaoFC,WuXF,WangFQ,ChenXJ,LiXY,GaoYL,ZhangYH,XiaoBG,WuWR.Mappingofquantitativetraitlociunderlyingsixagronomictraitsinflue-curedtobacco(NicotianatabacumL.).ActaAgronSin,2012,38:1–9 [4]NishiT,TajimaT,NoguchiS,AjisakaH,NegishiH.IdentificationofDNAmarkersoftobaccolinkedtobacterialwiltresistance.TheorApplGenet,2003,106:765–770 [5]JulioE,DenoyesRB,VerrierJL,deBorneFD.DetectionofQTLslinkedtoleafandsmokepropertiesinNicotianatabacumbasedonastudyof114recombinantinbredlines.MolBreed,2006,22:144–166 [6]VontimittaV,LewisRS.MappingofquantitativetraitlociaffectingresistancetoPhytophthoranicotianaeintobacco(NicotianatabacumL.)lineBeihart-1000.MolBreed,2010,58:294–300 [7]廖长见,王颖妲,潘光堂.作物染色体导入系的构建及其应用.分子植物育种,2007,5(6):139–144 LiaoCJ,WangYH,PanGT.Constructionandapplicationofchromosomeintrogressionlinesincrops.MolPlantBreed,2007,5(6):139–144(inChinesewithEnglishabstract) [8]JenaKK,KhushGS,KochertG.RFLPanalysisofrice(Oryzasatival)introgressionlines.TheorApplGenet,1992,84:608–616 [9]EshedY,AbiedM,SarangaY,ZamirD.Lycopersiconesculentumlinescontainingsmalloverlappingintrogressionsfrompennellii.TheorApplGenet,1992,83:1027–1034 [10]EshedY,ZamIR.AnintrogressionlinepopulationofLycopersiconpennelliiinthecultivatedtomatoenablestheidentificationandfinemappingofyield-associatedQTL.Genetics,1995,14:1147–1162 [11]MonforteAJ,TanksleySD.DevelopmentofasetofnearisogenicandbackcrossrecombinantinbredlinescontainingmostoftheLycopersiconhirsutumgenomeinanL.esculentumgeneticbackground:Atoolforgenemappingandgenediscovery.Genome,2000,43:803–813 [12]BernacehiD,Beck-BunnT,EmmattyD,EshedY,InaiS,LoPezJ,PetiardV,SayamaH,U'hligJ,ZamirD,TanksleyS.AdvancedbackcrossQTLanalysisoftomato.II.Evaluationofnear-isogeniclinescarryingsingle-donorintrogressionsfordesirablewildQTL-allelesderivedfromLycopersiconhirsutumandL.pimpinellifolium.TheorApplGenet,1998,97:170–180 [13]RamsayLD,JenningsDE,BohuonEJR,ArihurAE,LydiateDJ,KearseyMJ,MarshallDF.TheconstructionofasubstitutionlibraryofrecombinantbackcrosslinesinBrassicaoleraceafortheprecisionmappingofquantitativetraitloci.Genome,1996,39:558–567 [14]JeukenMJW,LindhoutP.Thedevelopmentoflettucebackcrossinbredlines(BILs)forexploitationofthelaetucasaligna(wildlettuce)germplasm.TheroApplGenet,2004,109:394–401 [15]MatusL,CoreyA,FiliehkinT.Developmentandcharacterizationofrecombinantchromosomesubstitutionlines(RCSLs)usingHordeumvelgaresubsp.spontaneumasasourceofdonorallelesinaHordeumvulgarsubsp.velgarebackground.Genome,2003,46:10l0–1023 [16]KorffMV,WangH,LeonJ.Developmentofcandidateintrogressionlinesusinganexoticbarleyaccession(Hordeumvulgarsubsp.spontaneous)asdonor.TheorApplGenet,2004,109:1736–1745 [17]冯长辉,张胜昔,王志伟,王夏青,李夕梅.棉花染色体单片段导入系的研究进展.湖北农业科学,2009,48(12):24–26 FengCH,ZhangSX,WangZW,WangXQ,LiXM.ResearchprogressonconstructionandapplicationofSSILsincotton.HubeiAgriculturalSciences,2009,48(12):24–26(inChinesewithEnglishabstract) [18]孟佳丽,娄群峰,周晓慧,史建磊,陈劲枫.黄瓜-酸黄瓜染色体片段导入系群体的构建及果实相关数量性状基因的定位.中国农业科学,2012,45(8):1558–1567 MengJL,LouQF,ZhouXH,ShiJL,ChenJF.Constructionofcucumber-sourcucumberchromosomeintrogressionlinesandlocationoffruitrelatedQTLs.SciAgricSin,2012,45:1558–1567(inChinesewithEnglishabstract) [19]林肖剑,许学文,钱红梅,齐晓花,徐强,陈学好.黄瓜抗白粉病染色体片段导入系的SSR鉴定.园艺学报,2012,39:485–492 LinJX,XuXW,QianHM,QiXH,XuQ,ChenXH.AnalysisofcucumberchromosomesegmentintrogressionlinewithpowderymildewresistancebasedonSSRmarkers.ActaHortSin,2012,39:485–492(inChinesewithEnglishabstract) [20]史建磊,娄群峰,钱春桃,万红建,周晓慧,陈劲枫.黄瓜染色体片段导入系的构建与遗传评价.南京农业大学学报,2011,34(1):20–24 ShiJL,LouQF,QianCT,WanHJ,ZhouXH,ChenJF.Constructionandgeneticevaluationofchromosomesegmentintrogressionlinesincucumber.JNanjingAgricUniv,2011,34(1):20–24(inChinesewithEnglishabstract) [21]刘冠明,李文涛,曾瑞珍,张泽民,张桂权.水稻单片段代换系片段的QTL鉴定.遗传学报,2004,31:1395–1400 LiuGM,LiWT,ZengRZ,ZhangGQ.IdentificationofQTLsonsubstitutedsegmentsinsinglesegmentsubstitutionlinesofrice.ActaGenetSin,2004,31:1395–1400(inChinesewithEnglishabstract) [22]何风华,席章营,曾瑞珍,AkshayT,张桂权.利用高代回交和分子标记辅助选择建立水稻单片段代换系.遗传学报,2005,32:825–831 HeFH,XiZY,ZengRZ,AkshayT,ZhangGQ.Developingsinglesegmentsubstitutionlines(SSSls)inrice(OryzasativalL)usingadvancedbackcrossesandMAS.ActaGenetSin,2004,32:825–831(inChinesewithEnglishabstract) [23]LiZK,FuBY,GaoYN.Genome-wideintrogressionlinesandtheiruseingeneticandmoleculardissectionofcomplexphenotypesinrice(OryzasativaL.).PlantMolBiol,2005,59:33–52 [24]王玉民,席章营,尚爱兰,王帮太.作物单片段代换系的构建及应用.中国农学通报,2008,24(3):67–71 WangYM,XiZY,ShangAL,WangBT.Constructionandapplicationofsinglesegmentsubstitutionlinesincrops.ChinAgricSciBull,2008,24(3):67–71(inChinesewithEnglishabstract) [25]Bindler,G,derHoevenVR,GunduzI,PlieskeJ,GanalM,RossiL,GadaniF,DoniniP.Amicrosatellitemarkerbasedlinkagemapoftobacco.TheorApplGenet,2007,114:341–349 [26]Bindler,G,PlieskeJ,BakaherN,GunduzI,IvanovN,derHoevenRV,GanalM,DoniniP.Ahighdensitygeneticmapoftobacco(NicotianatabacumL.)obtainedfromlargescalemicrosatellitemarkerdevelopment.TheorApplGenet,2011,123:219–230 [27]Sierro,N,BatteyJND,OuadiS,BakaherN,BovetL,WilligA,GoepfertS,PeitschMC,IvanovNV.Thetobaccogenomesequenceanditscomparisonwiththoseoftomatoandpotato.Nat.Commun,2014,5:3833doi:10.1038/ncomms4833 [28]TongZJ,YangZM,ChenXJ,JiaoFC,LiXY,WuXF,GaoYL,XiaoBG,WuWR.Large-scaledevelopmentofmicrosatellitemarkersinNicotianatabacumandconstructionofageneticmapofflue-curedtobacco.PlantBreed,2012,131:674–680 [29]ArslanB,OkunusA.Geneticandgeographicpolymorphismofcultivatedtobaccos(Nicotianatabacum)inTurkey.PlantGenet,2006,42:667–671 [30]DelPianoL,AbetMC,SorrentinoF,AcanforaE,CozzolinoE,DiMuroA.GeneticvariabilityinNicotianatabacumandNicotianaspeciesasrevealedbyRAPDprocedure.IntlContributTobRes,2000,19:1–15 [31]MoonHS,NicholsonJS,LewisRS.UseoftransferableNicotianatabacumL.microsatellitemarkersforinvestigatinggeneticdiversityinthegenusNicotiana.Genome,2008,51:547–559 [32]MoonHS,NicholsonJS,HeinemanA,LionK,derHoevenRV,HayesAJ,LewisRS.ChangesingeneticdiversityofU.S.Flue-Curedtobaccogermplasmoversevendecadesofcultivardevelopment.CropSci,2009,49:498–506 [33]MoonHS,NifongJM,NicholsonJS,HeinemanA,LionK,derHoevenRV,HayesAJ,LewisRS.Microsatellite-basedanalysisoftobacco(NicotianatabacumL.)geneticresources.CropSci,2009,49:2149–2157 [34]BaiD,ReelederR,BrandleJE.IdentificationoftwoRAPDmarkerstightlylinkedwiththeNicotianadebneyigeneforresistancetoblackrootrotoftobacco.TheorApplGenet,1995,91:1184–1189 [35]MaguireT,CollinsG,SedgleyM.AmodifiedCTABDNAextractionprocedureforplantsbelongingtothefamilyProteaceae.PlantMolBiolRep,1994,12:106–109 [36]YongND,TanlalesySD.Restrictionfragmentpolymorphismmapsandtheconceptofgraphicalgenotypes.TheorApplGenet,1989,77:95–101 [37]张桂权,曾瑞珍,丁效华,张泽民,李文涛.水稻单片段代换系文库的构建及其在分子育种上的应用.见:方宣钧,黎志康主编.2005植物分子育种国际学术研讨会论文集.北京:科学出版社,2005.pp106–113 ZhangGQ,ZengRZ,DingXH,LiWT.Constructionofalibraryofsinglesegmentsubstitutionlinesforricebreedingbydesign.In:FangXJ,LiZK,eds.Proceedingsofthe2005InternationalConferenceonPlantMolecularBreeding.Beijing:SciencePress,2005.pp106-113

[1] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[2] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[3] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
[4] ZHENG Dong, ZHOU Xian-Li, TENG Chang-Cai, HOU Wan-Wei, ZHANG Hong-Yan, LIU Yu-Jiao. Genetic relationship analysis and fingerprints construction of faba bean varieties in Qinghai province based on SSR markers [J]. Acta Agronomica Sinica, 2025, 51(1): 79-90.
[5] KUANG Bo-Wen, WEI Ni, LIU Jin-Dian, CHEN Mei-Yan, MAO Xing-Jie, DUAN Wei-Xing, YANG Xi-Ping. Development of SSR markers and database based on genomes of sugarcane and its relatives [J]. Acta Agronomica Sinica, 2025, 51(1): 103-116.
[6] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, CAO Yue, CHEN Xi-Ming, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Core germplasm identification of proso millet in spring sowing area of Northeast China using fluorescent microsatellite markers [J]. Acta Agronomica Sinica, 2024, 50(7): 1728-1739.
[7] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, GUO Juan, Dipak K SANTRA, WANG Rui-Yun, CHEN Xi-Ming. Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China [J]. Acta Agronomica Sinica, 2024, 50(5): 1181-1192.
[8] TIAN Chun-Yan, BIAN Xin, LANG Rong-Bin, YU Hua-Xian, TAO Lian-An, AN Ru-Dong, DONG Li-Hua, ZHANG Yu, JING Yan-Fen. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane [J]. Acta Agronomica Sinica, 2024, 50(2): 310-324.
[9] CHEN Tian, LI Yu-Ying, RONG Er-Hua, WU Yu-Xiang. Character identification and floral organ transcriptome analysis on artificial allotetraploids of Gossypium hirsutum L. [J]. Acta Agronomica Sinica, 2024, 50(2): 325-339.
[10] CHEN Zhi-Kai, ZHOU Xian-Li, ZHANG Hong-Yan, TENG Chang-Cai, HOU Wan-Wei. SSR association analysis of the protein content of 320 faba bean germplasms [J]. Acta Agronomica Sinica, 2024, 50(11): 2775-2786.
[11] CAO Yue, Zhang Li-Yuan, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Construction of DNA molecular identity card of proso millet in Ningxia based on fluorescent SSR [J]. Acta Agronomica Sinica, 2024, 50(11): 2699-2711.
[12] GUO Juan, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Molecular identification of broomcorn millet germplasm resources in Shaanxi based on SSR [J]. Acta Agronomica Sinica, 2024, 50(10): 2643-2653.
[13] WANG Qian, ZHANG Li-Yuan, XU Yue, LI Hai, LIU Shao-Xiong, XUE Ya-Peng, LU Ping, WANG Rui-Yun, LIU Min-Xuan. High motif EST-SSR markers development and genetic diversity evaluation for 200 core germplasms in proso millet [J]. Acta Agronomica Sinica, 2023, 49(8): 2308-2318.
[14] CHEN Yi-Hang, TANG Chao-Chen, ZHANG Xiong-Jian, YAO Zhu-Fang, JIANG Bing-Zhi, WANG Zhang-Ying. Construction of core collection of sweetpotato based on phenotypic traits and SSR markers [J]. Acta Agronomica Sinica, 2023, 49(5): 1249-1261.
[15] LI Ying, LIU Hai-Cui, SHI Lyu, SHI Xiao-Xu, HAN Xiao, LIU Jian, WEI Ya-Feng. Genetic diversity and population structure analysis of naked barley germplasm resources in Jiangsu province [J]. Acta Agronomica Sinica, 2023, 49(10): 2687-2697.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!