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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 72-81.doi: 10.3724/SP.J.1006.2017.00072


Effect of Biochar on Root Morphogenesis and Anatomical Structure of Rice Cultivated in Cold Region of Northeast China

ZHOU Jin-Song1,2,YAN Ping2,ZHANG Wei-Ming1,ZHENG Fu-Yu2,CHENG Xiao-Yi1,CHEN Wen-Fu1,*   

  1. 1Rice Institute of Shenyang Agricultural University / Biochar Engineering Technology Research Center of Liaoning Province / Northeast Key Laboratory of Rice Biology and Genetic Breeding, Ministry of Agriculture / Northern Key Laboratory of Super Rice Breeding, Ministry of Education, Shenyang 110866, China; 2 Wuchang Rice Institute, Heilongjiang Academy of Agricultural Sciences, Wuchang 150229, China
  • Received:2016-06-11 Revised:2016-09-18 Online:2017-01-12 Published:2016-09-29
  • Contact: CHEN Wenfu,E-mail: wfchen5512@126.com E-mail:zhoujinsong168@126.com
  • Supported by:

    This study was supported by the State Key Special Program of Soil Fertility Improvement and Cropping Innovation for High Yield with High Efficiency in Rice Cropping Areas (2016YFD0300904), Liaoning Province Major Science and Technology Platform for University (Biochar Engineering and Technical Research Center), Shenyang Special Program for Apply Basic Research Program (F16-205-1-38), Special Fund for Agro-scientific Research in the Public Interest Program of China (201303095) and Ministry of Education Program for Innovative Research Team (IRT13079).


To explore potential and practical application value of biochar in rice production in the cold region of Northeast China, we added 0-20.0% (w/w) biochar in rice nursery substrate of paddy soil and studied the root morphogenesis and anatomical structure of rice at 30 days after seed germination, The treatment of 5.0% biochar significantly increased the root length, root surface area and root volume, with the maximum value for all the root morphological indexes in the treatment of 10.0% biochar. When the biochar added more than 10.0%, the root morphological indexes started to decrease. It was indicated that the increase of root length, root surface area and root volume was the consequence of producing more fine roots. Meanwhile, when 5.0% biochar added in the rice nursery substrate, root epidermis thickness, cortex thickness, sclerenchyma tissue, number of vessels, sectional area of vessels, cortex cavity area, sectional area of whole root and root radius increased in comparison with those of control. In the treatment of 5.0%-10.0% biochar addition, all the indexes of anatomical structure of root reached the maximum. When the biochar added more than 10.0%, all the indexes of anatomical structure of root had a descending trend. It was revealed that the well-developed root epidermis and cortex were the main reason of promoting enlargement of roots when a moderate amount of biochar was added in the rice nursery substrate. Taken together, we conclude that the proper addition of 5.0%-10.0% (w/w) biochar is advantageous to the elongation, enlargement and formation of well-developed root system ,resulting in improved quality of rice seedlings in the dry rice-nursery of protected area when the paddy soil is used as the nursery substrate in the cold region of Northeast China.

Key words: Biochar, Rice (Oryza sativa L.), Root morphology, Anatomical structure

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