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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (04): 522-532.doi: 10.3724/SP.J.1006.2018.00522


QTL Analysis of Under-ear Internode Length Based on SSSL Population

Hai-Ping GUO1(), Gao-Yang SUN1, Xiao-Xiang ZHANG2, Peng-Shuai YAN1, Kun LIU1, Hui-Ling XIE1, Ji-Hua TANG1, Dong DING1, Wei-Hua LI1,*()   

  1. 1 College of Agronomy, Henan Agricultural University / Key Laboratory of Wheat and Maize Crops Science, Zhengzhou 450002, Henan, China
    2 Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, Sichuan, China
  • Received:2017-06-14 Accepted:2017-11-21 Online:2018-04-12 Published:2017-12-01
  • Contact: Wei-Hua LI E-mail:guohaiping885@126.com;liwh416@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (U1604231), the Major Science and Technology Projects in Henan Province (161100110500), and Henan Science and Technology Project (152102110060).


The under-ear internode length determines maize plant height and ear height, which are two agronomic traits associated with yield and lodging resistance. The lengths of the 7th, 8th, and 9th internode play a decisive role in ear height. Compared with other agronomic traits, there is little knowledge for genetic basis of under-ear internode length. Thus, exploring the genetic basis of internode length plays an important role in maize breeding. In this study, a set of 260 chromosome segment substitution lines (CSSLs), using Chang 7-2 as the donor parent and lx9801 as recipient parent, was constructed and used to map QTLs for the 7th, 8th, and 9th internode length and ear height at two-environments in two years. In total, 18, 23, and 17 QTLs were detected for the 7th, 8th, and 9th internode length, respectively. Among them, eight QTLs were simultaneously detected for the 7th, 8th, and 9th internode length. For ear height, 20 QTLs were detected, 12 (60%) of these 20 QTLs were found to co-localize to the 7th, 8th, and 9th internode length. The results indicated that length of the 7th, 8th, and 9th internode and ear height have same genetic basis. Furthermore, length of the 7th, 8th, and 9th internode are important components of ear height and also determining the plant height and ear height in maize.

Key words: maize, single segment substitution lines (SSSL), under-ear internode length, ear height, quantitative trait loci (QTL)

Table 1

performance of sevnth,eighth,and ninth internode length and car height for parents and SSSL population(cm)"

Table 2

Result of t-test of 7th, 8th, 9th internode length, and ear height for parents"

2012 2013
浚县 Xunxian 长葛 Changge 浚县 Xunxian 长葛 Changge
第7节间长7th internode length 4.047** 3.036** 0.045* 0.874**
第8节间长8th internode length 0.626** 2.145** 2.443** 2.991**
第9节间长9th internode length 6.024** 8.374** 2.967** 6.889**
穗位高Ear height 1.171** 2.609* 2.369* 2.085**

Table 3

Correlation coefficients analysis of seventh, eighth, ninth internode length with ear height in SSSL population"

浚县 Xunxian 长葛 Changge
7th IL
8th IL
9th IL
7th IL
8th IL
9th IL
2012 第7节间长7th IL 1 1
第8节间长8th IL 0.760** 1 0.633** 1
第9节间长9th IL 0.597** 0.635** 1 0.558** 0.538** 1
穗位高EH 0.432** 0.455** 0.439** 1 0.415** 0.443** 0.431** 1
2013 第7节间长7th IL 1 1
第8节间长8th IL 0.684** 1 0.645** 1
第9节间长9th IL 0.680** 0.677** 1 0.364** 0.482** 1
穗位高EH 0.398** 0.417** 0.403** 1 0.386** 0.412** 0.393** 1

Table 4

QTLs detcced for seventh,eighth,ninth internode length and ear height in the SSSL population(cm)"

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