不同种植方式大豆根际土壤细菌多样性分析

doi:10.3724/SP.J.1006.2018.01539

Abstract

Abstract:

To study bacterial community structure of soybean rhizosphere soil in rotation and continuous cropping, 16S rDNA gene were sequenced of soil samples infected soybean cyst nematode from Heilongjiang Province two regions by the second generation of high-throughput sequencing Illumina MiSeq platform. A total of 25 419 OTUs were obtained from six soil samples and classified into 47 phylum, 147 class, and 709 genera. Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes were dominant bacteria, accounting for more than 90% of all soil bacterial communities. The total OTUs and richness were the highest in four years continuous cropping and the lowest in twenty years continuous cropping. The difference of bacteria abundance in different rotational cropping years was not obvious (P > 0.05), but abundance and adversity were significant in continuous cropping (P < 0.05). The relative abundance of Actinobacteria in different rotations was lower than that in continuous cropping, and the relative abundance of Gemmatimonadetes and Bacteroidetes was higher than that in the same area. The relative abundance of soil functional bacteria Bradyrhizobium, Streptomyces, Bacillus, Lysobacter, and Pedomicrobium were higher in different continuous cropping years than those in rotations. Predominant bacterial abundance in long term continuous cropping was more similar with that in rotational cropping.

Key words: soybean, rotation, continuous cropping, bacterial diversity, Illumina MiSeq sequencing

Fang WANG,Jing-Sheng CHEN,Da-Wei LIU. Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns[J].Acta Agronomica Sinica, 2018, 44(10): 1539-1547.

Figures/Tables 8

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样品 Sample	有效序列数量 Effective sequence-number	OTUs数量 OTUs number (97%)	Chao指数 Chao index	Shannon指数 Shannon index
感病品种-大麻-抗病品种(大庆) RR (Daqing)	39530	4243	5247.7 a	10.45 a
感病品种-大麻-感病品种(大庆) RS (Daqing)	37614	4140	5122.7 a	10.57 a
玉米-谷子-感病品种(齐齐哈尔) RQ (Qiqihar)	54387	4229	4972.7 a	10.20 b

样品 Sample	有效序列数量 Effective sequence-number	OTUs数量 OTUs number (97%)	Chao指数 Chao index	Shannon指数 Shannon index
连作4年(大庆) C4 (Daqing)	67271	4928	5659.6 a	10.47 a
连作10年(大庆) C10 (Daqing)	69356	4173	4583.8 b	10.23 b
连作20年(齐齐哈尔) C20 (Qiqihar )	52387	3706	4147.0 b	10.09 c

Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns

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