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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (8): 1279-1285.doi: 10.3724/SP.J.1006.2019.94001

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Activity and gene family expression of β-amylase in Brassica napus differing in harvest index

JIN Shu-Rong1,2,WANG Yan-Mei1,2,CHANG Yue1,2,WANG Yue-Hua1,2,LI Jia-Na1,2,NI Yu1,2,*()   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2 Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2019-01-01 Accepted:2019-04-15 Online:2019-08-12 Published:2019-05-08
  • Contact: Yu NI E-mail:nmniyu@126.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31771694);the Chongqing Basic and Advanced Research Project(cstc2018jcyjAX0263);the Chongqing Basic and Advanced Research Project(cstc2016jcyjA0170);the Fundamental Research Funds for the Central Universities(XDJK2017B028);the China Agriculture Research System(CARS-12)

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Abstract:

The transferring efficiency of photosynthate from source organs to grains is the key process to increase the harvest index in Brassica napus, and amylase activity in source organs affects the transport intensity of assimilates to grains. The relationship between β-amylase (BAM) and the formation of high harvest index in rapeseed had not been clear. In this study, three different inbred lines, high-yield and high-harvest index rapeseed, high-yield and low-harvest index rapeseed, and low-yield and low-harvest index rapeseed were selected, and stem, leaf, silique pericarp and seed were sampled at 5, 10, 15, 20, and 25 d after the final flowering to analyze the activity of β-amylase and the expression level of its gene family members. The activity of β-amylase increased with the development of source organs. The activity of β-amylase in leaves and silique pericarps of high-harvest index rapeseed was significantly higher than that of low-harvest index rapeseed. In the β-amylase gene family, the expression of BAM1, BAM4, and BAM5 in the stem, leaf and silique pericarp of rapeseed increased with the development of organs. At 25 d after the final flowering, the expression of BAM1 and BAM3 in leaves and silique pericarps of high-harvest index rapeseed was significantly higher than that of low harvest index rapeseed. The expression of BAM4 and BAM5 in the silique pericarps of rapeseed with high harvest index was significantly higher than that of rapeseed with low harvest index at 15 d and 20 d after the final flowering. Taken together, BAM1 and BAM3 may enhance the transport intensity of photosynthate to grains by promoting starch degradation in leaves and silique pericarps in rapeseed with high harvest index, and BAM4 and BAM5 may regulate the transport of photosynthate to grains mainly by acting on starch degradation in silique pericarps. BAM4 and BAM5 may also be involved in the regulation of starch in rapeseed seeds.

Key words: Brassica napus, harvest index, β-amylase;, gene expression

Table 1

Sequences of the primers used in qPCR"

名称
Name		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)		 扩增片段大小
Size (bp)
BAM1 ATTGGAGCTTTCCAGTGCTATGACA GCCAGTTATTGTAGTGACCAGCATCG 124
BAM3 GATGTGACTCTGTGCCTGTCCTCA CTCCACAAGGTCCCATTCCCA 141
BAM4 ATTCTTCTGGTTGCCCTATCATTAAA GACAGAAACGCTCCACAATCCC 126
BAM5 CAGATCATAGGAGAAGCCAACAAGA GCGTGGCTGTGGTGGTTGTA 101
Actin7 GTGACAATGGAACTGGAATGGTGA GTGCCTAGGACGACCAACAATACTC 92

Fig. 1

Biological yield and harvest index in different B. napus varieties HH: high-yield and high-harvest index rapeseed; HL: high-yield and low-harvest index rapeseed; LL: low-yield and low-harvest index rapeseed; HI: harvest index. Different letters above the column indicate significant difference at P < 0.05 among varieties."

Fig. 2

Dynamics of β-amylase activity in different B. napus parts of plant at different growth stages after flowering"

Fig. 3

Expression of β-amylase gene families in stem of B. napus at different growth stages after flowering 缩写同图1。Abbreviations are the same as those given in Fig. 1."

Fig. 4

Expression of β-amylase gene families in leaf of B. napus at different growth stages after flowering 缩写同图1。Abbreviations are the same as those given in Fig. 1."

Fig. 5

Expression of β-amylase gene families in silique pericarp of B. napus at different growth stages after flowering 缩写同图1。Abbreviations are the same as those given in Fig. 1."

Fig. 6

Expression of β-amylase gene families in seed of B. napus at different growth stages after flowering 缩写同图1。Abbreviations are the same as those given in Fig. 1."

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