外源物质浸种对迟播油菜越冬期抗寒性及产量的影响

doi:10.3724/SP.J.1006.2024.34134

Abstract

Abstract:

In double-cropping rice areas in the Yangtze River Basin (YRB), ensuring the yield of late-seeded rapeseed is essential to reduce the winter fallow fields. Additionally, promoting the accumulation of dry matter before winter and improving cold resistance during the overwintering period are effective ways to increase the yield of late-seeded rapeseed. Soaking seeds with exogenous substances is an effective measure to improve the cold resistance during the overwintering period and promote the rapid growth before winter. In this experiment, the early maturing rapeseed variety Huayouza 137 was selected, while soaking-seed treatments of water (CK); 0.01 mmol L^-1, 0.05 mmol L^-1, 0.10 mmol L^-1 betaine (T1-1, T1-2, T1-3); 0.1 mmol L^-1, 0.5 mmol L^-1, 1.0 mmol L^-1 proline (T2-1, T2-2, T2-3); 0.03%, 0.15%, 0.30% hydrogen peroxide (T3-1, T3-2, T3-3); 0.001 mmol L^-1, 0.01 mmol L^-1, 0.05 mmol L^-1 malic acid (T4-1, T4-2, T4-3); 25 mg L^-1, 100 mg L^-1, 300 mg L^-1 nano zinc oxide (T5-1, T5-2, T5-3); 0.5 mmol L^-1, 1.0 mmol L^-1 polyamines (T6-1, T6-2) were conducted between 2021 and 2023 rapeseed growing season. We studied the effects of soaking seeds with different exogenous substances and their levels on the cold resistance in winter and yield of late-seeded rapeseed. The results showed that the different exogenous substances and their levels of soaking seeds affected the germination rate of late-seeded rapeseed, and part of them, such as T3-3, T4-2, and T5-3, significantly increased by 19.2%, 15.3%, and 17.3% versus CK. Soaking seeds with some exogenous substances significantly improved the cold resistance of late-seeded rapeseed in winter. On the one hand, the activities of peroxidase, catalase, and the content of glutathione were improved, and the contents of hydrogen peroxide, active oxygen and MDA reduced; on the other hand, the contents of soluble sugar, proline, malic acid, polyamine, and mannitol were increased; at the same time, the content of membrane cold response protein kinase was increased. The increase of leaf cold resistance was beneficial to the accumulation of leaf biomass and the increase of effective branches and pod number per plant, thus promoting yield. In addition, 9 positive and 3 negative cold resistance indicators were comprehensively evaluated. The results showed that the two-year average yield ranking and the comprehensive evaluation value ranking of cold resistance were basically the same over the two years. While the soaking-seed treatments with the best yield and cold resistance were T5-3, T5-2, T2-3, and T1-2. These results of this study provide theoretical and technical support for the cultivation of late-seeded rapeseed seedlings before winter and the improvement of cold resistance in the YRB, and provide a basis for the stress resistance and stable production of late-seeded rapeseed and the development and utilization of winter fallow fields.

Key words: rapeseed, late-seeded, cold resistance, exogenous substance, soaking seeds

WANG Xian-Ling, JIANG Yue, LEI Yi-Zhong, XIAO Sheng-Nan, SHE Hui-Jie, DUAN Sheng-Xing, HUANG Ming, KUAI Jie, WANG Bo, WANG Jing, ZHAO Jie, XU Zheng-Hua, ZHOU Guang-Sheng. Effects of seed soaking with exogenous substances on late-seeded rapeseed cold resistance of during overwintering period and yield[J].Acta Agronomica Sinica, 2024, 50(5): 1271-1286.

Figures/Tables 13

Table 1

Fig. 1

Fig. 2

Table 2

Effects of soaking seeds with different exogenous substances on yield and yield components of late-seeded rapeseed"

年份 Year	外源物质 Exogenous substance	浓度 Concentration	单株角果数 Pod number per plant	每角粒数 Seed number per pod	千粒重1000-seed weight (g)	单株产量 Yield per plant (g)	成株率Survival rate (%)	产量 Yield (kg hm^-2)
2021- 2022	对照CK	CK	128.7 ef	17.40 bc	3.55 ab	7.95 bc	45.27 d	2347.1 gh
	甜菜碱 Betaine (T1)	T1-1	125.1 ef	17.84 a	3.40 abc	7.61 bc	47.56 bcd	2112.9 i
		T1-2	145.8 cde	17.31 c	3.60 a	9.09 ab	46.95 bcd	2558.7 abc
		T1-3	146.3 cde	17.68 ab	3.44 abc	8.89 abc	47.33 bcd	2549.6 abc
	脯氨酸 Proline (T2)	T2-1	139.8 cdef	17.66 ab	3.51 abc	8.66 abc	47.18 bcd	2563.1 abc
		T2-2	178.7 a	17.48 abc	3.37 bc	10.53 a	49.68 abc	2571.5 abc
		T2-3	163.4 abc	17.42 bc	3.43 abc	9.75 ab	49.81 abc	2591.8 abc
	过氧化氢 Hydrogen peroxide (T3)	T3-1	114.5 f	17.34 bc	3.45 abc	6.87 c	49.82 abc	1771.8 j
		T3-2	126.7 ef	17.69 ab	3.52 abc	7.89 bc	47.17 bcd	2321.5 h
		T3-3	134.1 def	17.46 abc	3.34 c	7.83 bc	47.58 bcd	2430.2 efg
	苹果酸 Malic acid (T4)	T4-1	136.1 def	17.26 c	3.47 abc	8.13 bc	49.65 abc	2444.2 ef
		T4-2	141.5 cdef	17.38 bc	3.48 abc	8.57 abc	49.92 abc	2451.6 def
		T4-3	138.4 cdef	17.66 ab	3.51 abc	8.56 abc	44.65 d	2419.8 fg
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	159.8 abcd	17.32 bc	3.48 abc	9.66 ab	45.74 cd	2536.1 bcd
		T5-2	163.8 abc	17.53 abc	3.34 c	9.60 ab	52.43 a	2603.6 ab
		T5-3	171.7 ab	17.45 abc	3.51 abc	10.52 a	50.00 ab	2635.1 a
	多胺 Polyamine (T6)	T6-1	148.8 bcde	17.39 bc	3.44 abc	8.90 abc	47.21 bcd	2451.3 def
	多胺 Polyamine (T6)	T6-2	135.1 def	17.67 ab	3.44 abc	8.20 bc	47.15 bcd	2510.4 cde
2022- 2023	对照CK	CK	126.0 de	17.19 b	3.54 ab	7.66 cde	47.09 ab	2280.0 e
	甜菜碱 Betaine (T1)	T1-1	122.0 de	17.44 ab	3.37 bc	7.20 de	50.45 a	2050.5 f
		T1-2	139.4 cd	17.73 a	3.58 a	8.85 abcd	48.46 ab	2508.4 a
		T1-3	141.9 bcd	17.67 a	3.33 bc	8.37 bcde	45.45 ab	2449.9 abcd
	脯氨酸 Proline (T2)	T2-1	133.0 d	17.16 b	3.47 abc	7.90 cde	50.05 ab	2482.5 abc
		T2-2	174.2 a	17.52 ab	3.45 abc	10.54 a	46.19 ab	2488.5 ab
		T2-3	160.7 abc	17.62 ab	3.31 c	9.38 abc	48.96 ab	2532.2 a
2022- 2023	过氧化氢 Hydrogen peroxide (T3)	T3-1	108.3 e	17.44 ab	3.50 ab	6.62 e	46.27 ab	1671.1 g
		T3-2	123.7 de	17.64 ab	3.50 ab	7.63 cde	46.81 ab	2261.2 e
		T3-3	129.4 de	17.74 a	3.30 c	7.56 cde	48.36 ab	2366.2 bcde
	苹果酸 Malic acid (T4)	T4-1	129.5 de	17.39 ab	3.53 ab	7.97 cde	47.89 ab	2355.1 de
		T4-2	134.1 d	17.56 ab	3.53 ab	8.33 bcde	47.61 ab	2359.4 cde
		T4-3	134.7 d	17.57 ab	3.48 abc	8.21 bcde	49.18 ab	2339.2 de
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	157.2 abc	17.37 ab	3.45 abc	9.44 abc	50.37 ab	2453.1 abcd
		T5-2	159.2 abc	17.48 ab	3.48 abc	9.70 abc	47.66 ab	2525.9 a
		T5-3	164.1 ab	17.55 ab	3.52 ab	10.14 ab	47.83 ab	2575.0 a
	多胺 Polyamine (T6)	T6-1	146.8 bcd	17.44 ab	3.44 abc	8.81 abcd	48.40 ab	2358.4 cde
	多胺 Polyamine (T6)	T6-2	129.6 de	17.36 ab	3.51 abc	7.89 cde	45.10 b	2454.8 abcd
方差分析 ANOVA
年份 Year			*	ns	ns	ns	ns	**
处理 Treatment			**	ns	ns	**	ns	**
年份×处理 Year×Treatment			ns	ns	ns	ns	ns	ns

Table 2

Table 3

Effects of soaking seeds with different exogenous substances on key agronomic characters of late-seeded rapeseed at maturity"

年份 Year	外源物质 Exogenous substance	浓度 Concentration	株高 Plant height (cm)	有效分枝数Effective branch number	根颈粗 Root-crown diameter (mm)	地上部干重 Shoot biomass (g)	抗折力 Bending resistance (N)
2021-2022	对照CK	CK	164.1 ab	4.6 bcd	10.8 bcd	23.6 abc	79.2 g
	甜菜碱 Betaine (T1)	T1-1	171.5 ab	4.5 bcd	11.4 abcd	22.6 bc	64.2 h
		T1-2	170.8 ab	5.9 a	10.0 d	29.7 abc	96.4 cd
		T1-3	168.6 ab	5.6 abc	11.9 abcd	29.7 abc	95.4 cd
2021-2022	脯氨酸 Proline (T2)	T2-1	174.6 a	5.6 ab	12.1 abc	30.0 abc	96.4 cd
		T2-2	164.2 ab	5.1 abcd	11.1 abcd	30.7 ab	91.8 de
		T2-3	170.4 ab	5.4 abcd	12.7 ab	31.1 ab	115.6 a
	过氧化氢 Hydrogen peroxide (T3)	T3-1	166.8 ab	4.8 abcd	11.4 abcd	20.7 c	82.4 efg
		T3-2	172.3 ab	5.3 abcd	12.8 a	23.5 abc	91.6 de
		T3-3	157.8 b	5.2 abcd	11.1 abcd	24.7 abc	102.2 bc
	苹果酸 Malic acid (T4)	T4-1	173.1 a	5.5 abc	12.5 ab	24.8 abc	81.4 fg
		T4-2	166.9 ab	4.9 abcd	12.2 abc	26.3 abc	94.4 cd
		T4-3	169.6 ab	4.7 bcd	10.5 cd	24.7 abc	92.6 cd
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	170.5 ab	4.4 cd	11.1 abcd	29.1 abc	81.4 fg
		T5-2	170.3 ab	4.2 d	10.5 cd	32.4 a	94.4 cd
		T5-3	166.3 ab	4.3 d	11.2 abcd	32.5 a	92.6 cd
	多胺 Polyamine (T6)	T6-1	173.7 a	5.5 abc	12.2 abc	27.2 abc	107.0 ab
	多胺 Polyamine (T6)	T6-2	170.8 ab	4.8 abcd	11.1 abcd	27.6 abc	89.4 def
2022-2023	对照CK	CK	166.3 ab	4.8 bcd	10.4 abc	22.6 abc	69.2 g
	甜菜碱 Betaine (T1)	T1-1	164.6 ab	4.4 d	10.3 bc	22.2 abc	54.2 h
		T1-2	172.0 ab	4.5 cd	11.0 abc	20.9 bc	86.4 bcd
		T1-3	171.4 ab	6.0 a	9.4 c	29.0 ab	85.4 cd
	脯氨酸 Proline (T2)	T2-1	169.1 ab	5.7 abc	11.2 abc	28.7 ab	86.4 bcd
		T2-2	175.0 a	5.7 ab	11.5 ab	28.6 ab	81.8 de
		T2-3	164.7 ab	5.2 abcd	10.6 abc	30.8 a	95.6 ab
	过氧化氢 Hydrogen peroxide (T3)	T3-1	171.0 ab	5.5 abcd	12.3 a	29.5 ab	72.4 efg
		T3-2	166.9 ab	4.9 abcd	10.5 abc	19.4 c	81.6 de
		T3-3	172.5 ab	5.4 abcd	12.1 ab	22.5 abc	92.2 abc
	苹果酸 Malic acid (T4)	T4-1	158.3 b	5.3 abcd	10.7 abc	23.9 abc	71.4 fg
		T4-2	173.7 ab	5.6 abc	11.8 ab	23.7 abc	84.4 cd
		T4-3	167.4 ab	4.9 abcd	11.6 ab	25.7 abc	82.6 cd
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	170.2 ab	4.8 abcd	10.1 bc	23.7 abc	71.4 fg
		T5-2	170.9 ab	4.5 cd	10.3 bc	28.0 abc	84.4 cd
		T5-3	170.5 ab	4.3 d	10.2 bc	27.9 abc	82.6 cd
	多胺 Polyamine (T6)	T6-1	166.9 ab	4.3 d	10.6 abc	31.6 a	97.0 a
	多胺 Polyamine (T6)	T6-2	174.4 a	5.6 abc	11.7 ab	25.7 abc	79.4 def
方差分析 ANOVA
年份 Year			ns	ns	**	ns	**
处理 Treatment			ns	**	ns	**	**
年份×处理 Year×Treatment			**	ns	**	ns	ns

Table 3

Table 4

Effects of soaking seeds with different exogenous substances on key agronomic characters and leaf biomass of late-seeded rapeseed during wintering period"

年份 Year	外源物质 Exogenous substance	浓度 Concentration	株高 Plant Height (cm)	根长 Root length (cm)	根颈粗 Root-crown diameter (mm)	叶面积 Leaf area (cm²)	叶片生物量 Leaf biomass (g)
2021- 2022	对照CK	CK	22.4 b	12.2 abc	2.80 cde	436.6 de	1.24 gh
	甜菜碱 Betaine (T1)	T1-1	21.8 bc	11.9 bc	2.71 de	423.6 e	1.13 h
		T1-2	21.0 bc	13.4 abc	3.43 abcd	487.9 bcde	1.63 cd
		T1-3	21.0 bc	12.8 abc	3.37 abcde	481.1 bcde	1.55 de
	脯氨酸 Proline (T2)	T2-1	20.1 bc	14.1 a	3.57 abc	498.3 bcd	1.71 bc
		T2-2	20.8 bc	12.8 abc	3.58 abc	506.9 bc	1.73 bc
		T2-3	21.6 bc	12.9 abc	3.63 abc	507.1 bc	1.82 ab
	过氧化氢 Hydrogen peroxide (T3)	T3-1	21.4 bc	12.6 abc	2.57 e	326.0 f	0.77 i
		T3-2	21.7 bc	13.3 abc	2.80 cde	426.2 e	1.24 gh
		T3-3	22.0 bc	12.9 abc	2.99 bcde	448.7 cde	1.38 efg
	苹果酸 Malic acid (T4)	T4-1	23.9 a	12.6 abc	3.07 abcde	460.6 cde	1.41 ef
		T4-2	20.2 bc	13.1 abc	3.14 abcde	470.7 cde	1.46 e
		T4-3	21.4 bc	11.9 bc	2.91 bcde	446.2 cde	1.27 fgh
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	19.6 c	12.7 abc	3.27 abcde	476.0 bcde	1.49 e
		T5-2	20.6 bc	13.6 abc	3.69 ab	537.0 ab	1.89 a
		T5-3	21.1 bc	14.0 ab	3.84 a	571.7 a	1.93 a
	多胺 Polyamine (T6)	T6-1	20.7 bc	12.0 abc	3.10 abcde	470.1 cde	1.43 e
	多胺 Polyamine (T6)	T6-2	20.8 bc	11.4 c	3.23 abcde	473.3 bcde	1.48 e
2022- 2023	对照CK	CK	22.3 ab	12.4 bc	2.95 abc	435.7 cd	1.16 de
	甜菜碱 Betaine (T1)	T1-1	21.3 abc	12.3 bc	2.84 bc	413.6 d	1.02 e
		T1-2	20.3 bcd	14.3 abc	3.49 abc	481.2 bcd	1.46 bc
		T1-3	20.8 bcd	13.2 abc	3.50 abc	473.0 bcd	1.49 bc
	脯氨酸 Proline (T2)	T2-1	19.5 cd	14.9 a	3.68 abc	488.9 bc	1.63 ab
		T2-2	20.1 bcd	13.5 abc	3.78 ab	501.6 bc	1.61 ab
		T2-3	21.2 abcd	13.2 abc	3.73 ab	493.5 bc	1.72 a
	过氧化氢 Hydrogen peroxide (T3)	T3-1	21.0 bcd	13.1 abc	2.73 c	318.5 e	0.69 f
		T3-2	21.4 abc	13.5 abc	2.89 bc	416.8 d	1.16 de
		T3-3	21.5 abc	13.3 abc	3.17 abc	439.9 cd	1.21 de
	苹果酸 Malic acid (T4)	T4-1	23.1 a	12.8 abc	3.16 abc	446.5 cd	1.29 cd
		T4-2	20.0 bcd	13.6 abc	3.22 abc	454.9 bcd	1.34 cd
		T4-3	20.9 bcd	12.5 bc	3.08 abc	438.0 cd	1.15 de
	氧化纳米锌 Nano zinc oxide (T5)	T5-1	18.9 d	13.2 abc	3.33 abc	471.3 bcd	1.38 cd
		T5-2	20.3 bcd	14.0 abc	3.79 ab	521.2 ab	1.75 a
		T5-3	20.9 bcd	14.4 ab	3.93 a	559.9 a	1.77 a
	多胺 Polyamine (T6)	T6-1	20.2 bcd	12.3 bc	3.23 abc	459.1 bcd	1.32 cd
	多胺 Polyamine (T6)	T6-2	20.4 bcd	12.0 c	3.41 abc	464.8 bcd	1.32 cd
方差分析 ANOVA
年份 Year			**	**	*	ns	**
处理 Treatment			**	**	**	**	**
年份×处理 Year×Treatment			ns	ns	ns	ns	ns

Table 4

Fig. 3

Table 5

Table 6

Table 7

Fig. 4

Fig. 5

Table 8

References 39

[1]	刘成, 冯中朝, 肖唐华, 马晓敏, 周广生, 黄凤洪, 李加纳, 王汉中. 我国油菜产业发展现状、潜力及对策. 中国油料作物学报, 2019, 41: 485-489. doi: 10.7505/j.issn.1007-9084.2019.04.001
	Liu C, Feng Z C, Xiao T H, Ma X M, Zhou G S, Huang F H, Li J N, Wang H Z. Development, potential and adaptation of Chinese rapeseed industry. Chin J Oil Crop Sci, 2019, 41: 485-489 (in Chinese with English abstract). doi: 10.7505/j.issn.1007-9084.2019.04.001
[2]	Food and Agriculture Organization FAO of the United Nations.FAO Statistical Databases in 2023. [2023-04-25]. http://www.fao.org.
[3]	李勤, 刘小焱, 盛紫微, 曲昭杰, 罗涛, 王晶, 蒯婕, 汪波, 李俊, 徐正华, 周广生. 我国油菜适合机械化收获关键农艺性状研究进展. 中国油料作物学报, 2023, 45: 1053-1061. doi: 10.19802/j.issn.1007-9084.2022194
	Li Q, Liu X Y, Sheng Z W, Qu Z J, Luo T, Wang J, Kuai J, Wang B, Li J, Xu Z H, Zhou G S. Research progress on target agronomic traits for mechanized harvesting of rapeseed in China. Chin J Oil Crop Sci, 2023, 45: 1053-1061 (in Chinese with English abstract). doi: 10.19802/j.issn.1007-9084.2022194
[4]	张顺凯, 王端, 陶雨佳, 江海东. H₂O₂浸种对晚直播油菜生长及产量的影响. 中国油料作物学报, 2019, 41: 559-567. doi: 10.7505/j.issn.1007-9084.2019.04.010
	Zhang S K, Wang D, Tao Y J, Jiang H D. Effects of seeds soaking with hydrogen peroxide on growth and yield of rapeseed at different sowing dates. Chin J Oil Crop Sci, 2019, 41: 559-567 (in Chinese with English abstract).
[5]	张钰钦, 杨之帆, 李越, 李银水, 胡小加, 秦璐, 廖星. 外源海藻糖浸种对低温胁迫油菜种子萌发及幼苗生长的影响. 中国油料作物学报, 2022, 44: 376-384. doi: 10.19802/j.issn.1007-9084.2020317
	Zhang Y Q, Yang Z F, Li Y, Li Y S, Hu X J, Qin L, Liao X. Effect of exogenous trehalose on seed germination and seedling growth of rapeseed under low temperature. Chin J Oil Crop Sci, 2022, 44: 376-384 (in Chinese with English abstract).
[6]	朱春权, 魏倩倩, 项兴佳, 胡文君, 徐青山, 曹小闯, 朱练峰, 孔亚丽, 刘佳, 金千瑜, 张均华. 褪黑素和茉莉酸甲酯基质育秧对水稻耐低温胁迫的调控作用. 作物学报, 2022, 48: 2016-2027. doi: 10.3724/SP.J.1006.2022.12041
	Zhu C Q, Wei Q Q, Xiang X J, Hu W J, Xu Q S, Cao X C, Zhu L F, Kong Y L, Liu J, Jin Q Y, Zhang J H. Regulation effects of seedling raising by melatonin and methyl jasmonate substrate on low temperature stress tolerance in rice. Acta Agron Sin, 2022, 48: 2016-2027 (in Chinese with English abstract).
[7]	刘自刚, 张长生, 孙万仓, 杨宁宁, 王月, 何丽, 赵彩霞, 武军艳, 方彦, 曾秀存. 不同生态区冬前低温下白菜型冬油菜不同抗寒品种(系)的比较. 作物学报, 2014, 40: 346-354. doi: 10.3724/SP.J.1006.2014.00346
	Liu Z G, Zhang C S, Sun W C, Yang N N, Wang Y, He L, Zhao C X, Wu J Y, Fang Y, Zeng X C. Comparison of winter rapeseed varieties (lines) with different cold resistance planted in the northern-extending regions in China under low temperature before winter. Acta Agron Sin, 2014, 40: 346-354 (in Chinese with English abstract). doi: 10.3724/SP.J.1006.2014.00346
[8]	蒲媛媛, 赵玉红, 武军艳, 刘丽君, 白静, 马骊, 牛早霞, 金姣姣, 方彦, 李学才, 孙万仓. 北方强冬性甘蓝型冬油菜品种(系)抗寒性评价. 中国农业科学, 2019, 52: 3291-3308. doi: 10.3864/j.issn.0578-1752.2019.19.002
	Pu Y Y, Zhao Y H, Wu J Y, Liu L J, Bai J, Ma L, Niu Z X, Jin J J, Fang Y, Li X C, Sun W C. Comprehensive assessment on cold tolerance of the strong winter Brassica napus L. cultivated in northern China. Sci Agric Sin, 2019, 52: 3291-3308 (in Chinese with English abstract).
[9]	曾韶西, 王以柔. 低温胁迫对黄瓜子叶抗坏血酸过氧化物酶活性和谷胱甘肽含量的影响. 植物生理学报, 1990, 16: 37-42.
	Zeng S X, Wang Y R. Effects of low temperature stress on ascorbic acid peroxidase activity and glutathione content in cucumber cotyledons. Acta Phytophy Sin, 1990, 16: 37-42 (in Chinese).
[10]	Chung S W, Rho H, Lim C K, Jeon M K, Kim S, Jang Y J, An H J. Photosynthetic response and antioxidative activity of ‘Hass’ avocado cultivar treated with short-term low temperature. Sci Rep, 2022, 12: 11593. doi: 10.1038/s41598-022-15821-3 pmid: 35804002
[11]	He H, Lei Y, Yi Z, Raza A, Zeng L, Yan L, Ding X Y, Yong C, Zou X L. Study on the mechanism of exogenous serotonin improving cold tolerance of rapeseed (Brassica napus L.) seedlings. Plant Growth Regul, 2021, 94: 161-170. doi: 10.1007/s10725-021-00700-0
[12]	Wang P C, Hsu C C, Du Y Y, Zhu P P, Zhao C Z, Fu X, Zhang C G, Paez J S, Macho A P, Tao W A, Zhu J K. Mapping proteome-wide targets of protein kinases in plant stress responses. Proc Nat Acad Sci USA, 2020, 117: 3270-3280. doi: 10.1073/pnas.1919901117
[13]	Hu S L, Chen Q H, Guo F, Wang M L, Zhao H, Wang Y, Ni D J, Wang P. (Z)-3-Hexen-1-ol accumulation enhances hyperosmotic stress tolerance in Camellia sinensis. Plant Mol Biol, 2020, 103: 287-302. doi: 10.1007/s11103-020-00992-2
[14]	张曼, 戴蓉, 张顺凯, 江海东. H₂O₂浸种对油菜种子低温萌发的缓解效应. 南京农业大学学报, 2017, 40: 963-970.
	Zhang M, Dai R, Zhang S K, Jiang H D. Alleviation effects of seed soaking with H₂O₂ on seed germination in rape under low temperature stress. J Nanjing Agric Univ, 2017, 40: 963-970 (in Chinese with English abstract).
[15]	方彦, 武军艳, 孙万仓, 杨晓娟, 韩慧敏, 韩亚伟, 陈亚平. 外源ABA浸种对冬油菜种子萌发及幼苗抗寒性的诱导效应. 干旱地区农业研究, 2014, 32(6): 70-74.
	Fang Y, Wu J Y, Sun W C, Yang X J, Han H M, Han Y W, Chen Y P. Inducing effects of exogenous ABA on seed germination and cold tolerance of winter rape seedlings. Agric Res Arid Areas, 2014, 32(6): 70-74 (in Chinese with English abstract).
[16]	黄少华, 王增春, 刘胜环. 不同植物生长调节剂浸种对油菜壮苗的效果比较. 江苏农业科学, 2006, (3): 49-51.
	Huang S H, Wang Z C, Liu S H. Comparison of the effect of soaking seeds with different plant growth regulators on rapeseed seedling strengthening. Jiangsu Agric Sci, 2006, (3): 49-51 (in chinese).
[17]	徐家裕, 倪文海. 早中熟甘蓝型油菜播种期研究. 中国油料作物学报, 1987, (2): 41-44.
	Xu J Y, Ni W H. Study on sower for early and late maturing Brassica napus L. Chin J Oil Crop Sci, 1987, (2): 41-44 (in Chinese).
[18]	万林. H₂O₂浸种对直播油菜生长和抗寒性的影响. 南京农业大学硕士学位论文, 江苏南京, 2015.
	Wan L. Effect of Seed Soaking with Hydrogen Peroxide on Growth and Chilling Resistance of Direct-seeding Oilrape. MS Thesis of Nanjing Agricultural University, Nanjing, Jiangsu, China (in Chinese with English abstract).
[19]	Ella E S, Dionisio-Sese M L, Ismail A M. Seed pre-treatment in rice reduces damage, enhances carbohydrate mobilization and improves emergence and seedling establishment under flooded conditions. AoB Plants, 2011, 2011: plr007.
[20]	Alabdallah N M, Alzahrani H S. The potential mitigation effect of ZnO nanoparticles on [Abelmoschus esculentus L. Moench] metabolism under salt stress conditions. Saudi J Biol Sci, 2020, 27: 3132-3137. doi: 10.1016/j.sjbs.2020.08.005 pmid: 33100874
[21]	Faizan M, Bhat J A, Hessini K, Yu F, Ahmad P. Zinc oxide nanoparticles alleviates the adverse effects of cadmium stress on Oryza sativa via modulation of the photosynthesis and antioxidant defense system. Ecotoxicol Environ Saf, 2021, 220: 112401. doi: 10.1016/j.ecoenv.2021.112401
[22]	Rameshraddy, Pavithra G J, Reddy B H R, Salimath M, Geetha K N, Shankar A G. Zinc oxide nano particles increases Zn uptake, translocation in rice with positive effect on growth, yield and moisture stress tolerance. Indian J Plant Physiol, 2017, 22: 287-294. doi: 10.1007/s40502-017-0303-2
[23]	Fatima A, Safdar N, Ain N, Yasmin A, Chaudhry G. Abscisic acid-loaded ZnO nanoparticles as drought tolerance inducers in Zea mays L. with physiological and biochemical aAttributes. J Plant Growth Regul, 2023, 42: 7280-7293. doi: 10.1007/s00344-023-11016-w
[24]	李春燕, 陈思思, 徐雯, 李东升, 顾骁, 朱新开, 郭文善, 封超年. 苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响. 作物学报, 2011, 37: 2293-2298. doi: 10.3724/SP.J.1006.2011.02293
	Li C Y, Chen S S, Xu W, Li D S, Gu X, Zhu X K, Guo W S, Feng C N. Effect of low temperature at seedling stage on antioxidation enzymes and cytoplasmic osmoticum of leaves in wheat cultivar yangmai 16. Acta Agron Sin, 2011, 37: 2293-2298 (in Chinese with English abstract). doi: 10.3724/SP.J.1006.2011.02293
[25]	Ren Y Q, Guo Y T, Zhao M L. Research progress of response to low temperature stress in Plant. Mol Plant Breed, 2020, 18: 4775-4781.
[26]	吕艳, 黄涌, 邹锡玲, 罗丹, 王小燕, 鲍五洲, 陈建军, 马海清, 程勇. 油菜抗低温的评价指标与分子生理机制研究进展. 中国油料作物学报, 2020, 42: 527-535.
	Lyu Y, Huang Y, Zou X L, Luo D, Wang X Y, Bao W Z, Chen J J, Ma H Q, Cheng Y. Reaserches on evaluation, physiological and molecular mechanism of rapeseed low-tempreature resistance. Chin J Oil Crop Sci, 2020, 42: 527-535 (in Chinese with English abstract). doi: 10.19802/j.issn.1007-9084.2020151
[27]	Jahed K R, Saini A K, Sherif S M. Coping with the cold: unveiling cryoprotectants, molecular signaling pathways, and strategies for cold stress resilience. Front Plant Sci, 2023, 14: 1246093. doi: 10.3389/fpls.2023.1246093
[28]	Zuo S Y, Zuo Y T, Gu W R, Wei S, Li J. Exogenous proline optimizes osmotic adjustment substances and active oxygen metabolism of maize embryo under low-temperature stress and metabolomic analysis. Processes, 2022, 10: 1388. doi: 10.3390/pr10071388
[29]	Chen Y, Jiang J F, Chang Q S, Gu C S, Song A P, Chen S M, Dong B, Chen F D. Cold acclimation induces freezing tolerance via antioxidative enzymes, proline metabolism and gene expression changes in two chrysanthemum species. Mol Biol, 2014, 41: 815-822.
[30]	Wu H C, Jinn T L. Heat shock-triggered Ca²⁺ mobilization accompanied by pectin methylesterase activity and cytosolic Ca²⁺ oscillation are crucial for plant thermotolerance. Plant Signal Behav, 2010, 5: 1252-1256. doi: 10.4161/psb.5.10.12607
[31]	Kang T M, Hilgemann D W. Multiple transport modes of the cardiac Na⁺/Ca²⁺ exchanger. Nature, 2004, 427: 544-548. doi: 10.1038/nature02271
[32]	Ren R F, Zhou H, Zhang L L, Jiang X R, Liu Y. Ca²⁺ participates in programmed cell death by modulating ROS during pollen cryopreservation. Plant Cell Rep, 2022, 41: 1043-1057. doi: 10.1007/s00299-022-02836-3
[33]	Renaut J, Hausman J F, Wisniewski M E. Proteomics and low-temperature studies: bridging the gap between gene expression and metabolism. Physiol Plant, 2006, 126: 97-109.
[34]	Breton G, Vazquez-tello A, Danyluk J, Sarhan F. Two novel intrinsic annexins accumulate in wheat membranes in response to low temperature. Plant Cell Physiol, 2000, 41: 177-184. pmid: 10795312
[35]	Wang J C, Liu X, Zhang A, Ren Y L, Wu F Q, Wang G, Xu Y, Lei C L, Zhu S S, Pan T, Wang Y F, Zhang H, Wang F, Tan Y Q, Wang Y P, Jin X, Luo S, Zhou C L, Zhang X, Liu J L, Wang S, Meng L Z, Wang Y H, Chen X, Lin Q B, Zhang X, Guo X P, Cheng Z J, Wang J L, Tian Y L, Liu S J, Jiang L, Wu C Y, Wang E T, Zhou J M, Wang Y F, Wang H Y, Wan J M. A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice. Cell Res, 2019, 29: 820-831. doi: 10.1038/s41422-019-0219-7 pmid: 31444468
[36]	Subrahmanyam D, Subash N, Haris A, Sikka A K. Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica, 2006, 44: 125-129.
[37]	刘海卿, 孙万仓, 刘自刚, 武军艳, 钱武, 王志江, 郭仁迪, 马骊, 侯献飞, 刘林波. 北方寒旱区白菜型冬油菜抗寒性与抗旱性评价及其关系. 中国农业科学, 2015, 48: 3743-3756. doi: 10.3864/j.issn.0578-1752.2015.18.018
	Liu H Q, Sun W C, Liu Z G, Wu J Y, Qian W, Wang Z J, Guo R D, Ma L, Hou X F, Liu L B. Evaluation of drought resistance and cold resistance and research of their relationship at seedling stage of winter rapeseed (Brassica campestris L.) in cold and arid regions in North China. Sci Agric Sin, 2015, 48: 3743-3756 (in Chinese with English abstract).
[38]	范军强, 武军艳, 刘丽君, 马骊, 杨刚, 蒲媛媛, 李学才, 孙万仓. 甘蓝型冬油菜气孔特性与抗寒性的关系. 中国农业科学, 2023, 56: 599-619. doi: 10.3864/j.issn.0578-1752.2023.04.002
	Fan J Q, Wu J Y, Liu L J, Ma L, Yang G, Pu Y Y, Li X C, Sun W C. Correlation between stomatal characteristics and cold resistance of Brassica napus L. Sci Agric Sin, 2023, 56: 599-619 (in Chinese with English abstract).
[39]	Su H, Sun H, Dong X, Chen P, Zhang X, Tian L, Liu X, Wang J. Did manure improve saline water irrigation threshold of winter wheat? A 3-year field investigation. Agric Water Manag, 2021, 258: 107203. doi: 10.1016/j.agwat.2021.107203

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

外源物质 Exogenous substance	浓度 Concentration	MDA (nmol g^-1)	H₂O₂ (μmol g^-1)	O₂∙^- (μg g^-1)	·OH (ng g^-1)	SOD (U)	POD (U)	CAT (U)	APX (U)
对照CK	CK	184.3 a	0.983 b	5.56 b	7.37 a	1730.1 g	2.64 def	589.3 d	0.86 e
甜菜碱 Betaine (T1)	T1-1	190.2 a	1.014 b	6.29 a	7.85 a	1727.4 g	2.48 f	569.8 d	1.17 cd
	T1-2	121.7 cde	0.682 cd	5.48 bc	5.08 cd	2463.3 cd	3.52 bc	968.2 b	1.10 d
	T1-3	128.6 cd	0.741 c	4.95 bcde	5.24 c	2319.4 cde	3.41 c	937.1 b	0.94 e
脯氨酸 Proline (T2)	T2-1	116.6 cde	0.614 d	4.74 cde	4.69 cde	2485.1 cd	3.72 abc	969.1 b	0.91 e
	T2-2	115.8 cde	0.589 d	5.33 bc	4.69 cde	2585.5 c	3.80 ab	982.7 b	1.35 ab
	T2-3	115.3 cde	0.589 d	4.49 def	4.67 cde	2938.6 b	3.83 ab	1002.2 b	1.43 a
过氧化氢 Hydrogen peroxide (T3)	T3-1	192.8 a	1.123 a	4.43 ef	7.96 a	1091.9 h	1.59 g	561.1 d	0.85 e
	T3-2	185.4 a	1.002 b	3.94 f	7.40 a	1730.1 g	2.56 ef	589.3 d	0.98 e
	T3-3	177.9 a	0.968 b	5.18 bcd	7.16 a	1855.0 fg	2.84 def	681.6 cd	0.97 e
苹果酸 Malic acid (T4)	T4-1	157.3 b	0.937 b	3.97 f	6.37 b	1998.9 efg	2.93 de	740.8 c	1.26 bc
	T4-2	155.3 b	0.918 b	6.17 a	6.29 b	2069.6 defg	2.95 de	772.9 c	1.12 cd
	T4-3	183.3 a	0.969 b	4.60 def	7.25 a	1768.1 fg	2.69 def	613.5 d	0.95 e
氧化纳米锌 Nano zinc oxide (T5)	T5-1	134.2 c	0.773 c	5.50 bc	5.27 c	2210.8 cdef	3.08 d	917.6 b	0.98 e
	T5-2	108.1 de	0.570 d	6.45 a	4.30 de	3047.2 ab	4.00 a	1013.8 b	1.15 cd
	T5-3	105.4 e	0.561 d	6.23 a	4.14 e	3313.4 a	4.11 a	1122.6 a	0.90 e
多胺 Polyamine (T6)	T6-1	138.5 c	0.891 b	5.33 bc	6.19 b	2199.9 cdef	3.00 de	780.6 c	1.21 cd
多胺 Polyamine (T6)	T6-2	135.2 c	0.781 c	4.77 cde	5.44 c	2191.8 cdef	3.00 de	891.4 b	1.15 cd

外源物质 Exogenous substance	浓度 Concentration	抗坏血酸 ASA (μmol g^-1)	类胡萝卜素 Carnosine (pmol g^-1)	谷胱甘肽 GSH (mg g^-1)
对照CK	CK	6.91 b	1.22 def	4.06 d
甜菜碱 Betaine (T1)	T1-1	5.76 c	1.19 ef	3.46 e
	T1-2	9.13 a	1.03 gh	4.88 bc
	T1-3	7.14 b	0.96 h	4.84 bc
脯氨酸 Proline (T2)	T2-1	7.66 b	1.17 f	5.07 ab
	T2-2	5.57 c	1.53 ab	5.14 ab
	T2-3	9.42 a	1.29 cde	5.23 ab
过氧化氢 Hydrogen peroxide (T3)	T3-1	8.88 a	1.10 fg	3.28 e
	T3-2	8.73 a	1.12 fg	3.47 e
	T3-3	6.86 b	1.33 cd	4.34 cd
苹果酸 Malic acid (T4)	T4-1	5.90 c	1.37 c	4.37 cd
	T4-2	5.51 c	1.29 cde	4.44 cd
	T4-3	7.22 b	1.51 ab	4.11 d
氧化纳米锌 Nano zinc oxide (T5)	T5-1	7.31 b	1.35 cd	4.83 bc
	T5-2	8.65 a	1.42 bc	5.48 a
	T5-3	5.84 c	1.30 cde	5.56 a
多胺 Polyamine (T6)	T6-1	6.86 b	1.38 c	4.79 bc
多胺 Polyamine (T6)	T6-2	9.77 a	1.56 a	4.81 bc

外源物质 Exogenous substance	浓度 Concentration	可溶性糖 Soluble sugar (mg g^-1)	脯氨酸 Proline (ng g^-1)	苹果酸 Malate (μg g^-1)	甜菜碱 Betaine (pg g^-1)	多胺 Polyamine (mg g^-1)	甘露醇 Mannitol (ng g^-1)
对照CK	CK	4.28 k	268.5 b	3.71 f	398.6 cd	5.69 ef	126.7 e
甜菜碱 Betaine (T1)	T1-1	4.11 l	211.7 c	3.40 f	475.9 ab	5.45 ef	125.0 e
	T1-2	5.15 ef	319.3 a	5.03 bcd	365.0 cde	9.24 ab	181.8 bcd
	T1-3	5.13 ef	308.8 a	4.82 cd	523.3 a	8.99 abc	177.6 bcd
脯氨酸 Proline (T2)	T2-1	5.27 d	318.0 a	5.06 bcd	319.2 e	9.31 ab	184.4 bcd
	T2-2	5.23 de	321.8 a	5.17 bc	338.5 de	9.71 a	186.4 bc
	T2-3	5.39 c	318.7 a	5.42 bc	383.0 cde	9.32 ab	187.9 bc
过氧化氢 Hydrogen peroxide (T3)	T3-1	4.05 l	256.6 b	2.89 g	488.2 ab	5.01 f	70.4 f
	T3-2	4.27 k	303.8 a	3.48 f	492.7 ab	5.52 ef	125.5 e
	T3-3	4.42 j	264.7 b	3.78 f	483.7 ab	5.97 ef	159.2 cd
苹果酸 Malic acid (T4)	T4-1	4.55 i	215.0 c	3.81 f	354.4 de	6.10 ef	161.4 cd
	T4-2	4.72 h	253.0 b	4.06 ef	473.0 ab	7.91 d	162.9 cd
	T4-3	4.41 j	267.7 b	3.67 f	325.8 e	5.79 ef	153.6 d
氧化纳米锌 Nano zinc oxide (T5)	T5-1	5.04 f	300.0 a	4.77 cd	388.4 cde	8.12 cd	177.1 bcd
	T5-2	5.60 b	330.9 a	5.59 ab	428.4 bc	10.02 a	201.8 ab
	T5-3	6.31 a	322.8 a	6.00 a	369.1 cde	10.04 a	215.5 a
多胺 Polyamine (T6)	T6-1	4.84 g	323.3 a	4.15 ef	513.9 a	6.61 e	165.8 cd
多胺 Polyamine (T6)	T6-2	5.04 f	264.7 b	4.45 de	512.7 a	8.45 bcd	168.7 cd

外源物质 Exogenous substance	评价对象 Evaluation object	正理想解距离D+ Positive ideal solution distance D+	负理想解距离D- Negative ideal solution distance D-	相对接近度C Relative proximity C	排序结果Sorting result	平均产量排序结果 Average yield Sorting result
对照CK	CK	2.618	1.350	0.340	15	15
甜菜碱 Betaine (T1)	T1-1	3.111	0.882	0.221	17	17
	T1-2	0.908	2.890	0.761	6	4
	T1-3	1.079	2.705	0.715	7	7
脯氨酸 Proline (T2)	T2-1	0.733	3.068	0.807	5	6
	T2-2	0.642	3.168	0.832	4	5
	T2-3	0.581	3.219	0.847	3	3
过氧化氢 Hydrogen peroxide (T3)	T3-1	3.659	0.377	0.093	18	18
	T3-2	2.765	1.349	0.328	16	16
	T3-3	2.340	1.624	0.410	13	13
苹果酸 Malic acid (T4)	T4-1	2.236	1.741	0.438	12	12
	T4-2	1.904	1.942	0.505	11	10
	T4-3	2.505	1.493	0.373	14	14
氧化纳米锌 Nano zinc oxide (T5)	T5-1	1.258	2.527	0.668	8	8
	T5-2	0.335	3.496	0.913	2	2
	T5-3	0.068	3.724	0.982	1	1
多胺 Polyamine (T6)	T6-1	1.737	2.192	0.558	10	11
多胺 Polyamine (T6)	T6-2	1.445	2.365	0.621	9	9

Effects of seed soaking with exogenous substances on late-seeded rapeseed cold resistance of during overwintering period and yield

RichHTML424

PDF

Abstract

Cite this article

share this article

Figures/Tables 13

References 39

Related Articles 15

Metrics

Comments

Recommended 0

浓度 Concentration	对照 Contrast (CK)	甜菜碱 Betaine (T1)	脯氨酸 Proline (T2)	过氧化氢 Hydrogen peroxide (T3)	苹果酸 Malic acid (T4)	氧化纳米锌 Nano zinc oxide (T5)	多胺 Polyamine (T6)
浓度-1 Concentration-1	—	0.01 mmol L^-1	0.1 mmol L^-1	0.03%	0.001 mmol L^-1	25 mg L^-1	0.5 mmol L^-1
浓度-2 Concentration-2	—	0.05 mmol L^-1	0.5 mmol L^-1	0.15%	0.01 mmol L^-1	100 mg L^-1	1.0 mmol L^-1
浓度-3 Concentration-3	—	0.10 mmol L^-1	1.0 mmol L^-1	0.30%	0.05 mmol L^-1	300 mg L^-1	—

[1]	CAO Xin-Yuan, DU Ming-Li, WANG Yu-Cheng, CHEN Xin-Hua, CHEN Jia-Xin, LING Xiao-Xia, HUANG Jian-Liang, PENG Shao-Bing, DENG Nan-Yan. Evaluation of annual yield gap and yield limiting facters in rice-rapeseed cropping system: an example from Wuxue city, Hubei province, China [J]. Acta Agronomica Sinica, 2024, 50(5): 1287-1299.
[2]	YAN Jin-Yao, SONG Yi, LU Zhi-Feng, REN Tao, LU Jian-Wei. Effect of phosphorus fertilizer rate on rapeseed yield and quality (Brassica napus L.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1668-1677.
[3]	YU Xin-Ying, WANG Chun-Yun, LI Da-Shuang, WANG Zong-Kai, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, ZHOU Guang-Sheng. Formation mechanism of yield stability in high-yielding rapeseed varieties [J]. Acta Agronomica Sinica, 2023, 49(6): 1601-1615.
[4]	FANG Ya-Ting, REN Tao, ZHANG Shun-Tao, ZHOU Xiang-Qi, ZHAO Jian, LIAO Shi-Peng, CONG Ri-Huan, LU Jian-Wei. Different effects of nitrogen, phosphorus and potassium fertilizers on oilseed rape yield and nutrient utilization between continuous upland and paddy-upland rotations [J]. Acta Agronomica Sinica, 2023, 49(3): 772-783.
[5]	NING Ning, MO Jiao, HU Bing, LI Da-Shuang, LOU Hong-Xiang, WANG Chun-Yun, BAI Chen-Yang, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, LI Xiao-Hua, JIA Cai-Hua, ZHOU Guang-Sheng. Comparative study on the processing quality of winter rape in different ecological zones of the Yangtze River valley [J]. Acta Agronomica Sinica, 2023, 49(12): 3315-3327.
[6]	YE Xiao-Lei, GENG Guo-Tao, XIAO Guo-Bin, LYU Wei-Sheng, REN Tao, LU Zhi-Feng, LU Jian-Wei. Effects of magnesium application rate on yield and quality in oilseed rape (Brassica napus L.) [J]. Acta Agronomica Sinica, 2023, 49(11): 3063-3073.
[7]	GONG Ruo-Lin, SONG Bo, YANG Zhi-Ye, LU Li-Jing, DONG Jun-Gang. Effects of sowing date and density on lodging resistance and yield of different rapeseed cultivars [J]. Acta Agronomica Sinica, 2023, 49(10): 2777-2792.
[8]	QIN Lu, HAN Pei-Pei, CHANG Hai-Bin, GU Chi-Ming, HUANG Wei, LI Yin-Shui, LIAO Xiang-Sheng, XIE Li-Hua, LIAO Xing. Screening of rapeseed germplasms with low nitrogen tolerance and the evaluation of its potential application as green manure [J]. Acta Agronomica Sinica, 2022, 48(6): 1488-1501.
[9]	LOU Hong-Xiang, JI Jian-Li, KUAI Jie, WANG Bo, XU Liang, LI Zhen, LIU Fang, HUANG Wei, LIU Shu-Yan, YIN Yu-Feng, WANG Jing, ZHOU Guang-Sheng. Effects of planting density on yield and lodging related characters of reciprocal hybrids in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(9): 1724-1740.
[10]	ZHANG Jian, XIE Tian-Jin, WEI Xiao-Nan, WANG Zong-Kai, LIU Chong-Tao, ZHOU Guang-Sheng, WANG Bo. Estimation of feed rapeseed biomass based on multi-angle oblique imaging technique of unmanned aerial vehicle [J]. Acta Agronomica Sinica, 2021, 47(9): 1816-1823.
[11]	GUO Qing-Yun, KUAI Jie, WANG Bo, LIU Fang, ZHANG Chun-Yu, LI Gen-Ze, ZHANG Yun-Yun, FU Ting-Dong, ZHOU Guang-Sheng. Effect of mixed-sowing of near-isogenic lines on the clubroot disease controlling efficiency in rapeseed [J]. Acta Agronomica Sinica, 2020, 46(9): 1408-1415.
[12]	Qing-Yun GUO, Bo WANG, Jie KUAI, Chun-Yu ZHANG, Gen-Ze LI, Hui-Xian KANG, Ting-Dong FU, Guang-Sheng ZHOU. Controlling efficiency against clubroot disease of rapeseed by mixed-cropping of susceptible and resistant cultivars [J]. Acta Agronomica Sinica, 2020, 46(5): 725-733.
[13]	DONG Jian-Ke, TU Wei, WANG Hai-Bo, YING Jing-Wen, DU Juan, ZHAO Xi-Juan, ZHAO Qing-Hao, HUANG Wei, CAI Xing-Kui, SONG Bo-Tao. Establishment of a high efficient method for chromosome doubling and exploration of cold-resistant resources in potato [J]. Acta Agronomica Sinica, 2020, 46(11): 1659-1666.
[14]	LYU Wei-Sheng, XIAO Fu-Liang, ZHANG Shao-Wen, ZHENG Wei, HUANG Tian-Bao, XIAO Xiao-Jun, LI Ya-Zhen, WU Yan, HAN De-Peng, XIAO Guo-Bin, ZHANG Xue-Kun. Effects of sowing and fertilizing methods on yield and fertilizer use efficiency in red-soil dryland rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2020, 46(11): 1790-1800.
[15]	HU Mao-Long, CHENG Li, GUO Yue, LONG Wei-Hua, GAO Jian-Qin, PU Hui-Ming, ZHANG Jie-Fu, CHEN Song. Development and application of the marker for imidazolinone-resistant gene in Brassica napus [J]. Acta Agronomica Sinica, 2020, 46(10): 1639-1646.