深水稻全生育期耐盐性状的QTL定位

doi:10.3724/SP.J.1006.2024.42007

Abstract

Abstract:

Salinity stress is a major constraint to rice production in many coastal regions, particularly in the salt-fresh water interface areas. Salt tolerance in rice is a complex trait that can be localized through quantitative trait loci (QTL) mapping, which can facilitate the breeding of rice varieties with enhanced salt tolerance. In this study, an F₉ mapping population consisting of 174 recombinant inbred lines was developed from a cross between the coastal deep-water rice variety Chihe (donor parent) and the U.S. rice variety Lemont (receptor parent). The population was subjected to salinity stress during the germination, seedling, and reproductive stages with NaCl concentrations of 15 g L^-1, 5 g L^-1, and 5-6 g L^-1, respectively. Data were collected on relative germination rates, seedling salt tolerance grades, and seven phenotypic traits during the reproductive stages. Linkage genetic mapping and QTL mapping were performed using 142 simple sequence repeat (SSR) markers. The results indicated that Chihe was salt-sensitive at the germination stage but exhibited salt tolerance at the seedling and reproductive stages, while Lemont lines were consistently salt-sensitive. Approximately 70.11%, 50.57%, and 60.34% of the lines showed salt sensitivity in the germination, seedling, and reproductive stages, respectively, with a weak negative correlation of salt tolerance across the stages. A total of 33 QTLs were identified, with LOD values ranging from 2.52 to 10.32 and phenotypic variation explained ranging from 0.06% to 13.68%. Specifically, 4 QTLs were identified for the germination stage, 6 for the seedling stage, and 23 for the reproductive stage. Four overlapping QTLs were identified at the reproductive stage, and the QTLs contributing to the largest phenotypic variation were all derived from the salt-tolerant parent. Further investigation of these QTLs can provide new genetic resources for improving salt tolerance in rice breeding, thereby aiding the development of rice varieties with enhanced tolerance to salinity stress.

Key words: whole growth period, salt tolerance, deep-water rice, identification and evaluation, quantitative trait locus (QTL)

XIA Xiu-Zhong, ZHANG Zong-Qiong, NONG Bao-Xuan, FENG Rui, GUO Hui, CHEN Can, LIANG Shu-Hui, ZHUANG Jie, LIAO Zu-Yu, SONG Guo-Xian, YANG Xing-Hai, LI Dan-Ting. QTL mapping for salt tolerance traits throughout the entire growth period of deep-water rice[J].Acta Agronomica Sinica, 2024, 50(10): 2493-2502.

Figures/Tables 6

Fig. 1

Fig. 2

Table 1

Fig. 3

Fig. 4

Table 2

Associated QTL identified for salt tolerance at three rice growth period"

生长期 Growth period	QTL	分子标记 Marker interval	位置 Position (cM)	LOD值 LOD value	加性效应Additive effect	显性效应Dominant effect	贡献率 R²
芽期 Germination stage	qGR-7.1	RM11-RM10	55.17	10.32	1.037	1.914	13.68
	qGR-7.2	RM10-RM3826	69.77	7.88	0.923	2.060	11.33
	qGR-7.3	RM3286-RM505	73.91	2.55	-0.429	2.134	1.32
	qGR-10	RM8201-RM596	38.16	3.29	-0.659	1.206	2.40
苗期 Seedling stage	qSDI-1	RM259-RM572	45.84	3.34	-0.224	-1.206	0.98
	qSDI-2	RM525-RM1092	119.43	2.60	0.429	0.575	3.48
	qSDI-4	RM335-RM5687	23.30	2.59	0.533	-2.083	5.98
	qSDI-7.1	RM125-RM214	30.51	2.96	-0.515	-0.400	5.22
	qSDI-7.2	RM8263-RM11	34.50	4.31	-0.683	-0.029	9.65
	qSDI-10	RM6128-RM6824	46.81	2.62	0.460	-1.561	4.02
生殖生长期 Reproductive stage	qPH-2	RM3355-RM263	88.53	3.77	0.006	0.174	0.77
	qPH-8	RM223-RM210	76.28	3.18	-0.005	0.072	0.59
	qPH-9	RM215-RM205	64.98	3.20	-0.010	0.206	2.64
	qTN-4	RM5687-RM273	58.40	2.64	-0.029	0.346	4.53
	qTN-5.1	RM169-RM289	29.75	3.17	0.006	-0.123	0.20
	qTN-5.2	RM274-RM1054	88.17	2.67	-0.044	0.020	8.83
	qFGN-5	RM405-RM413	5.92	3.11	0.006	0.191	0.31
	qFGN-7	RM505-RM3589	76.91	2.52	0.019	0.063	3.45
	qSSR-5	RM413-RM5994	9.92	3.04	-0.005	0.274	0.12
	qSSR-10	RM216-RM311	12.17	2.58	-0.031	0.122	5.78
	qPY-2	RM5764-RM7581	9.65	2.54	0.029	0.040	4.48
	qPY-3	RM16-RM227	108.01	2.68	-0.035	-0.013	6.24
	qPY-5	RM413-RM5994	11.92	2.83	-0.012	0.306	0.74
	qPY-9	RM201-RM215	57.47	2.61	-0.038	-0.018	8.02
	qTGW-2.1	RM5764-RM7581	1.00	3.20	0.021	-0.002	8.01
	qTGW-2.2	RM3355-RM263	90.53	2.57	0.001	0.280	0.06
	qTGW-3.1	RM545-RM517	5.01	4.78	-0.004	0.219	0.35
	qTGW-3.2	RM517-OSR13	11.11	4.14	-0.005	0.281	0.46
	qTGW-4	RM5687-RM273	48.40	4.10	0.003	0.280	0.23
	qTGW-6	RM3827-RM5371	77.46	2.80	0.004	0.188	0.31
	qTGW-12.1	RM19-RM453	20.60	3.76	-0.015	0.088	4.13
	qTGW-12.2	RM453-RM7003	27.44	4.47	-0.012	0.143	2.67
	qTGW-12.3	RM309-RM235	71.71	2.96	-0.003	0.208	0.15

Table 2

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处理 Treatment	性状 Trait	亲本Parentage		重组自交系Recombinant inbred lines
处理 Treatment	性状 Trait	赤禾Chihe	Lemont	均值 Mean	标准差 SD	变异系数CV	最小值Min.	最大值Max.
正常对照 Control	株高 PH (cm)	224.5	96.8	130.04^*	25.28	0.19	83.33	185.00
	有效穗 TN	7.6	9.3	10.41	2.44	0.23	6.33	18.00
	穗长 SL (cm)	29.5	16.8	26.25	2.93	0.11	18.83	35.67
	实粒数 FGN	170.6	115.8	124.32^**	127.58	0.34	141.00	801.00
	结实率 SSR (%)	89.4	82.5	74.12^**	10.96	0.15	44.95	97.95
	单株产量 PY (g)	14.7	12.8	7.90^**	2.57	0.33	3.10	17.06
	千粒重 TGW (g)	23.1	26.8	21.55	3.17	0.15	15.27	30.88
盐胁迫 Salt treatment	株高 PH (cm)	194.6	86.2	123.12	27.94	0.23	67.40	194.60
	有效穗 TN	6.4	7.8	10.45	2.44	0.23	5.80	18.40
	穗长 SL (cm)	25.7	15.5	26.15	2.91	0.11	17.93	33.27
	实粒数 FGN	113.5	53.2	110.70	93.86	0.28	138.00	587.00
	结实率 SSR (%)	59.5	37.9	0.69	0.13	0.19	34.46	92.26
	单株产量 PY (g)	11.2	3.0	6.87	2.00	0.29	2.45	12.38
	千粒重 TGW (g)	19.8	23.2	20.99	3.62	0.17	13.04	38.90

QTL mapping for salt tolerance traits throughout the entire growth period of deep-water rice

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