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Functional study of the phosphate transporter protein BnaPT48 in Brassica napus

ZHANG Wen,LI Yu,WANG Chuang,SHI Lei,DING Guang-Da*#br#

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  1. College of Resources and Environment, Huazhong Agricultural University / Microelement Research Center / Key Laboratory of Arable Land Conservation in the Middle and Lower Reaches of Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China
  • Received:2025-04-18 Revised:2025-08-13 Accepted:2025-08-13 Published:2025-08-19
  • Supported by:
    This study was supported by the National Key R&D Program for Agricultural Biological Breeding (2023ZD04072).

Abstract:

Phosphorus (P) is an essential macronutrient required for plant growth and development. Rapeseed (Brassica napus), a major oilseed crop in China, is characterized by a high demand for P and strong sensitivity to P deficiency. This study investigates the gene BnaPT48, which is highly expressed in the root system of Brassica napus and is strongly induced under P-deficient conditions. Through analyses of its expression pattern, protein subcellular localization, and phenotypes of transgenic materials, the role of BnaPT48 in P uptake was elucidated. BnaPT48 was found to localize to the plasma membrane and was significantly upregulated by P deficiency in various root tissues. The BnaPT48 protein complemented the P uptake defect of the Arabidopsis thaliana mutant atpt1/2, indicating its functional role in enhancing P acquisition. Overexpression of BnaPT48 in A. thaliana promoted plant growth under both normal and low P conditions. In rapeseed, overexpression of BnaPT48 significantly increased the shoot dry weight under low P treatment, reduced inorganic P concentration in cotyledons, accelerated chlorophyll degradation and cotyledon senescence, and increased inorganic P accumulation in roots under normal P conditions. These findings reveal the function of BnaPT48 in regulating phosphate uptake and redistribution, and provide a theoretical foundation and genetic resource for improving P use efficiency in B. napus through genetic approaches.


Key words: Brassica napus, BnaPT48, transport protein, low phosphorus stress, P concentration, P absorption

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