Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (11): 1862-1868.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Genetic Differentiation of Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis Makino) Germplasm Based on SRAP Markers

HAN Jian-Ming12,HOU Xi-Lin1*,XU Hai-Ming3,SHI Gong-Jun1,GENG Jian-Feng1,DENG Xiao-Hui1   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu; 2 Luoyang Academy of Agricultural Sciences, Luoyang 471022, Henan; 3 Department of Agronomy, Zhejiang University, Hangzhou 310029, Zhejiang, China
  • Received:2006-10-12 Revised:1900-01-01 Online:2007-11-12 Published:2007-11-12
  • Contact: HOU Xi-Lin

Abstract:

The genetic diversity and differentiation were assessed in 64 accessions of non-heading Chinese cabbage from all the world by SRAP (Sequence-related amplified polymorphism) markers. Two hundred and fifteen loci were detected by 21 pairs of SRAP primers. Among them, 112 were polymorphic, the polymorphic rate was 52.09%, and 10.24 loci and 5.33 polymorphic loci were amplified by each pair primer an average. The Nei’s gene diversity index and Genetic richness of B. compestris var. communis were 0.1410 and (190) 88.37% respectively, the highest among different types of non-heading Chinese cabbage, and 0.1451 and (185) 86.05% respectively in Jianghuai Plain, the highest among all the regions zeeted. These two indices were respectively 0.1293 and (188) 87.44% higher in domestic cabbage than in the foreign cabbage. The measurement of genetic variation showed that the coefficient of genetic differentiation was 58.22%. Most of the genetic variation existed among populations. The gene flow (0.4031) was less among populations, indicating that the degree of genetic differentiation was higher. Six groups were clustered according to genetic similarity coefficient of 0.872.

Key words: Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), Germplasm, SRAP, Genetic diversity, Genetic differentiation

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