Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica

   

Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage

Wang Fei-Fei1,Zhang Sheng-Zhong1,Yang Gui-Hua2,Miao Hua-Rong1,Hu Xiao-Hui1,Zhang Ze-Lin3,Liu Sha-Sha4,Qiao Li-Xian5,Shan Shi-Hua1,*,Chen Jing1,*   

  1. 1 Shandong Peanut Research Institute, Qingdao 266100, Shandong, China; 2 Jinan Agricultural Technology Extension Center, Jinan 250002, Shandong, China; 3 Youzha Town People’s Government, Luanzhou City, Tangshan 063702, Hebei, China; 4 Qingdao Lujufeng Seed Industry Co., Ltd., Qingdao 266200, Shandong, China; 5 College of Agronomy, Qingdao Agriculture University, Qingdao 266109, Shandong, China
  • Received:2025-07-11 Revised:2025-10-30 Accepted:2025-10-30 Published:2025-11-10
  • Supported by:
    This study was supported by the Shandong Province Agriculture Improved Seed Project (2024LZGC031) and the Innovation Project of Shandong Academy of Agriculture Sciences (CXGC2025E02).

PDF

15

Abstract: Soil salinization is a major constraint limiting the expansion and yield of peanut cultivation. Screening salt-tolerant peanut germplasm and identifying related traits provide a foundation for breeding salt-tolerant varieties and investigating the mechanisms underlying salt tolerance. In this study, the salt tolerance of 331 peanut accessions was evaluated at the seedling stage using a hydroponic system. Eight parameters were measured: SPAD value, plant height, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, fresh weight root/shoot ratio, and dry weight root/shoot ratio. A comprehensive evaluation of salt tolerance was conducted using principal component analysis (PCA), membership function analysis, and cluster analysis. Under different salt concentrations, six parameters—plant height, shoot fresh weight, root fresh weight, shoot dry weight, fresh weight root/shoot ratio, and dry weight root/shoot ratio—showed significant differences among accessions. Salt stress inhibited peanut growth and significantly reduced four of these parameters compared with the control. Correlation analysis revealed significant relationships among the salt tolerance coefficients of all eight parameters, with the strongest correlation observed between root fresh weight and root dry weight (r = 0.83). PCA reduced the eight parameters to three principal components, accounting for a cumulative variance of 76.22%. Based on D-values (comprehensive evaluation scores), cluster analysis grouped the 331 accessions into five categories: Group I (highly salt-tolerant, 11 accessions), Group II (salt-tolerant, 33 accessions), Group III (intermediate, 104 accessions), Group IV (salt-sensitive, 42 accessions), and Group V (highly salt-sensitive, 141 accessions). A stepwise regression analysis yielded a predictive equation for evaluating salt tolerance in peanut seedlings: Y = 0.032 + 0.163X4 + 0.137X3 + 0.073X1 ? 0.158 X2 + 0.111X5 + 0.08X6. The D-value proved effective for assessing salt tolerance at the seedling stage, and 11 salt-tolerant germplasms, including Huashi 2, AM-Ceorganic, and Zhonghua 6, were identified. Plant height, root dry weight, and shoot dry weight were identified as key indicators for evaluating peanut salt tolerance at the seedling stage.

Key words: peanut, seedling, assessment of salt tolerance, principal component analysis, subordinate function analysis, germplasm evaluation

[1] Liang X Y, Li J F, Yang Y Q, et al. Designing salt stress-resilient crops: current progress and future challenges. J Integr Plant Biol, 2024, 66: 303–329.

[2] Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol, 2008, 59: 651–681.

[3] Ray D K, Mueller N D, West P C, et al. Yield trends are insufficient to double global crop production by 2050. PLoS One, 2013, 8: e66428.

[4] Li X G, Pang Y T, Zhong Y W, et al. GmGSTU23 encoding a tau class glutathione S-transferase protein enhances the salt tolerance of soybean (Glycine max L.). Int J Mol Sci, 2023, 24: 5547.

[5] 杨劲松. 中国盐渍土研究的发展历程与展望. 土壤学报, 2008, 45: 837–845.

Yang J S. Development and prospect of the research on salt-affected soils in China. Acta Pedol Sin, 2008, 45: 837–845 (in Chinese with English abstract).

[6] 乔钲岩, 于淼, 唐玉劼, 等. 粒用高粱幼苗期耐苏打盐碱综合评价与指标鉴选. 中国农业科学, 2025, 58: 30–49.

Qiao Z Y, Yu M, Tang Y J, et al. Comprehensive evaluation for soda salinity and alkalinity in sorghum seedling stage and selection of indicators. Sci Agric Sin, 2025, 58: 30–49 (in Chinese with English abstract).

[7] 高建明, 夏卜贤, 袁庆华, 等. 高粱种质材料幼苗期耐盐碱性评价. 应用生态学报, 2012, 23: 1303–1310.

Gao J M, Xia B X, Yuan Q H, et al. Salt-alkaline tolerance of sorghum germplasm at seedling stage. Chin J Appl Ecol, 2012, 23: 1303–1310 (in Chinese with English abstract).

[8] Dargan K S, Abrol I P, Bhumbla D R. Performance of rice varieties in a highly saline sodic soil as influenced by plant population. Agron J, 1974, 66: 279–280.

[9] 韩毅强, 高亚梅, 杜艳丽, 等. 大豆耐盐碱种质资源鉴定. 中国油料作物学报, 2021, 43: 1016–1024.

Han Y Q, Gao Y M, Du Y L, et al. Identification of saline-alkali tolerant germplasm resources of soybean during the whole growth stage. Chin J Oil Crop Sci, 2021, 43: 1016–1024 (in Chinese with English abstract).

[10] 刘永惠, 沈一, 陈志德, 等. 不同花生品种(系)萌发期耐盐性的鉴定与评价. 中国油料作物学报, 2012, 34: 168–173.

Liu Y H, Shen Y, Chen Z D, et al. Identification of salt tolerance in peanut varieties/lines at the germination stage. Chin J Oil Crop Sci, 2012, 34: 168–173 (in Chinese with English abstract).

[11] 李晓婷, 胡畅丽, 李鑫, 等. 花生萌发期耐盐性鉴定及耐盐种质筛选. 花生学报, 2022, 51(4): 35–43.

Li X T, Hu C L, Li X, et al. Identification and screening of salt-tolerance peanut cultivars during germination stage. J Peanut Sci, 2022, 51(4): 35–43 (in Chinese with English abstract).

[12] 苗华荣, 杨伟强, 胡晓辉, 等. 盐胁迫下花生幼苗期相关性状的QTL分析. 华北农学报, 2018, 33(3): 113–118.

Miao H R, Yang W Q, Hu X H, et al. QTL analysis on seedling stage traits of peanut under salt stress. Acta Agric Boreali-Sin, 2018, 33(3): 113–118 (in Chinese with English abstract).

[13] 王亮, 王睿, 朱金成, 等. 50份花生品种幼苗期耐盐性分析. 作物杂志, 2025, (5): 35: 35–41.

Wang L, Wang R, Zhu J C, et al. Analysis on salt tolerance of 50 peanut varieties at seedling stage. Crops, 2025, (5): 35: 35–41 (in Chinese with English abstract).

[14] 李晶晶, 王金秀, 曹议丹, 等. 高油酸花生萌发期和苗期耐盐碱性鉴定及评价. 中国农学通报, 2024, 40(15): 44–52.

Li J J, Wang J X, Cao Y D, et al. Peanut with high oleic acid content: identification and evaluation of salt-alkaline tolerance during germination and seedling stage. Chin Agric Sci Bull, 2024, 40(15): 44–52 (in Chinese with English abstract).

[15] 孙东雷, 卞能飞, 陈志德, 等. 花生萌发期耐盐性综合评价及耐盐种质筛选. 植物遗传资源学报, 2017, 18: 1079–1087.

Sun D L, Bian N F, Chen Z D, et al. Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of peanut (Arachis hypogaea L.) at germination stage. J Plant Genet Resour, 2017, 18: 1079–1087 (in Chinese with English abstract).

[16] 沈一, 刘永惠, 陈志德, 等. 花生幼苗期耐盐品种的筛选与评价. 花生学报, 2012, 41(1): 10–15.

Shen Y, Liu Y H, Chen Z D, et al. Selection and evaluation of peanut varieties based on seedling salt tolerance. J Peanut Sci, 2012, 41(1): 10–15 (in Chinese with English abstract).

[17] 慈敦伟, 丁红, 张智猛, 等. 花生耐盐性评价方法的比较与应用. 花生学报, 2013, 42(2): 28–35.

Ci D W, Ding H, Zhang Z M, et al. Comparison and application of different evaluation methods on peanut salt tolerance. J Peanut Sci, 2013, 42(2): 28–35 (in Chinese with English abstract).

[18] 陈杨, 吕玉英, 杨会, 等. 水培法鉴定花生苗期耐盐性研究. 山东农业科学, 2019, 51(9): 125–131.

Chen Y, Lyu Y Y, Yang H, et al. Study on salt tolerance identification of peanut at seedling stage by hydroponics. Shandong Agric Sci, 2019, 51(9): 125–131 (in Chinese with English abstract).

[19] 段雅娟, 曹士亮, 于滔, 等. 玉米自交系萌发期耐盐性鉴定. 作物杂志, 2022, (1): 213–219.

Duan Y J, Cao S L, Yu T, et al. Identification of salt tolerance during germination of maize inbred lines. Crops, 2022, (1): 213–219 (in Chinese with English abstract).

[20] 温刘君, 朴顺姬, 易津. 4种小麦族牧草种子耐盐补偿生长特性研究. 中国草地学报, 2009, 31(6): 30–32.

Wen L J, Piao S J, Yi J. Studies on the salt tolerance compensatory growth characteristics of four triticeae accessions’ seeds. Chin J Grassland, 2009, 31(6): 30–32 (in Chinese with English abstract).

[21] 白玉娥. 根茎类禾草耐盐性评价及生理基础的研究. 内蒙古农业大学博士学位论文, 内蒙古呼和浩特, 2004.

Bai Y E. Assessment of Dalt Tolerance and Physiology Basis of Rhizomatose Grass. PhD Dissertation of Inner Mongolia Agricultural University,

Hohhot, Inner Mongolia, China, 2004 (in Chinese with English abstract).

[22] 马晓军, 金峰学, 杨姗, 等. 作物耐盐碱数量性状基因座(QTL)定位. 分子植物育种, 2015, 13: 221–227.

Ma X J, Jin F X, Yang S, et al. Mapping QTLs for salt & alkaline tolerance in crops. Mol Plant Breed, 2015, 13: 221–227 (in Chinese with English abstract).

[1] YIN Li-Na, ZHANG Rui, CHEN Guo-Huan, BAI Lei, LI Jun, GUO Hua-Chun, YANG Fang. Comparison of wound healing capacity of tubers of different potato varieties [J]. Acta Agronomica Sinica, 2025, 51(9): 2399-2411.
[2] HU Run-Hui, WANG Jun-Cheng, SI Er-Jing, ZHANG Hong, LI Xing-Mao, MA Xiao-Le, MENG Ya-Xiong, WANG Hua-Jun, LIU Qing, YAO Li-Rong, LI Bao-Chun. Screening of drought and salt tolerant germplasm during wheat seedling stage and comprehensive evaluation of drought and salt tolerance [J]. Acta Agronomica Sinica, 2025, 51(9): 2371-2386.
[3] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[4] GAO Meng-Juan, ZHAO He-Ying, CHEN Jia-Hui, CHEN Xiao-Qian, NIU Meng-Kang, QIAN Qi-Run, CUI Lu-Fei, XING Jiang-Min, YIN Qing-Miao, GUO Wen, ZHANG Ning, SUN Cong-Wei, YANG Xia, PEI Dan, JIA Ao-Lin, CHEN Feng, YU Xiao-Dong, REN Yan. Mapping and identification of a novel sharp eyespot resistance locus Qse.hnau-5AS and its candidate genes in wheat [J]. Acta Agronomica Sinica, 2025, 51(8): 2240-2250.
[5] CAI Jin-Shan, LI Chao-Nan, WANG Jing-Yi, LI Ning, LIU Yu-Ping, JING Rui-Lian, LI Long, SUN Dai-Zhen. Genome-wide association study of root traits in wheat seedlings and identification of a superior allele at TaSRL-3B [J]. Acta Agronomica Sinica, 2025, 51(8): 2020-2032.
[6] WAN Shu-Bo, ZHANG Jia-Lei, GAO Hua-Xin, WANG Cai-Bin. Advances and prospects of high-yield peanut cultivation in China [J]. Acta Agronomica Sinica, 2025, 51(7): 1703-1711.
[7] GUO Teng-Da, CUI Meng-Jie, CHEN Lin-Jie, HAN Suo-Yi, GUO Jing-Kun, WU Chen-Di, FU Liu-Yang, HUANG Bing-Yan, DONG Wen-Zhao, ZHANG Xin-You. Cloning and expression analysis of the phosphatidylinositol transfer protein AhSFH gene in peanuts responsive to Aspergillus flavus infection [J]. Acta Agronomica Sinica, 2025, 51(6): 1489-1500.
[8] LI Wen-Jia, LIAO Yong-Jun, HUANG Lu, LU Qing, LI Shao-Xiong, CHEN Xiao-Ping, JIN Jing-Wei, WANG Run-Feng. Genome-wide associate analysis of flowering traits and identification of candidate genes in peanut [J]. Acta Agronomica Sinica, 2025, 51(5): 1400-1408.
[9] LIN Wei-Jin, GUO Ze-Jia, LIU Hao, LI Hai-Fen, WANG Run-Feng, HUANG Lu, YU Qian-Xia, CHEN Xiao-Ping, HONG Yan-Bin, LI Shao-Xiong, LU Qing. QTL mapping and candidate gene analysis of peanut pod yield-related traits [J]. Acta Agronomica Sinica, 2025, 51(4): 969-981.
[10] CHI Xiao-Yuan, BI Jing-Nan, ZHAO Jian-Xin, CHEN Na, PAN Li-Juan, JIANG Xiao, YIN Xiang-Zhen, ZHAO Xu-Hong, MA Jun-Qing, XU Jing. Evaluation of mechanical properties of peanut pods and screening of early maturing germplasm [J]. Acta Agronomica Sinica, 2025, 51(4): 943-957.
[11] JIN Gao-Rui, WU Xiao-Li, DENG Li, CHEN Yu-Ning, YU Bo-Lun, GUO Jian-Bin, DING Ying-Bin, LIU Nian, LUO Huai-Yong, CHEN Wei-Gang, HUANG Li, ZHOU Xiao-Jing, HUAI Dong-Xin, TAN Jia-Zhuang, JIANG Hui-Fang, REN Li, LEI Yong, LIAO Bo-Shou. Development and characterization of novel peanut genetic stocks with high oleic acid and enhanced resistance both to Aspergillus flavus infection and aflatoxin production [J]. Acta Agronomica Sinica, 2025, 51(3): 687-895.
[12] JIN Xin-Xin, SONG Ya-Hui, SU Qiao, YANG Yong-Qing, LI Yu-Rong, WANG Jin. Identification and comprehensive evaluation of drought resistance in high oleic acid Jihua peanut varieties [J]. Acta Agronomica Sinica, 2025, 51(3): 797-811.
[13] WANG Run-Feng, LI Wen-Jia, LIAO Yong-Jun, LU Qing, LIU Hao, LI Hai-Fen, LI Shao-Xiong, LIANG Xuan-Qiang, HONG Yan-Bin, CHEN Xiao-Ping. Evaluation of pod maturity and identification of early-maturing germplasm for core peanut germplasm resources [J]. Acta Agronomica Sinica, 2025, 51(2): 395-404.
[14] HU Peng-Ju, GUO Song, SONG Ya-Hui, JIN Xin-Xin, SU Qiao, YANG Yong-Qing, WANG Jin. Genetic and QTL mapping analysis of oil content in peanut across multiple environments [J]. Acta Agronomica Sinica, 2025, 51(2): 324-333.
[15] ZHAO Fei-Fei, LI Shao-Xiong, LIU Hao, LI Hai-Fen, WANG Run-Feng, HUANG Lu, YU Qian-Xia, HONG Yan-Bin, CHEN Xiao-Ping, LU Qing, CAO Yu-Man. Association mapping of internode and lateral branch internode length of peanut main stem and analysis of candidate genes [J]. Acta Agronomica Sinica, 2025, 51(2): 548-556.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd