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Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (7): 1949-1958.doi: 10.3724/SP.J.1006.2025.41075

• RESEARCH NOTES • Previous Articles     Next Articles

Screening of low nitrogen tolerant germplasm in seedling highland barley based on tolerance index and comprehensive evaluation of different nitrogen efficiency types

WEN Xuan1,2,ZHONG Xiu-Li1,2,WANG Shang-Wen1,2,JIN Tao3,4,PENG Jun3,LIU En-Ke1,2,3,*   

  1. 1 Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 State Key Laboratory of Efficient Utilization of Agricultural Water Resources, Beijing 100081, China; 3 Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, Xizang, China; 4 State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, Xizang, China
  • Received:2024-11-07 Revised:2025-03-26 Accepted:2025-03-26 Online:2025-07-12 Published:2025-04-01
  • Supported by:
    This study was supported by the the Project of the State Key Laboratory of Genetic Improvement and Germplasm Resources of Barley and Yak Co-built by the Ministry and the Province (XZNKY-CZ-2022-016-04), National Key Research and Development Program Project (2022YFD2301302-2), and Xizang Autonomous Region Science and Technology Program’s Project Selection through Public Challenge (XZ202101ZY0008N-KT02-Z05).

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Abstract:

Nitrogen (N) is an essential nutrient for crop growth and development; however, its deficiency in dryland soils often limits the growth and yield of highland barley. Identifying germplasm with low-N tolerance and high N-use efficiency is critical for improving nitrogen utilization. In this study, a hydroponic experiment was conducted using 143 highland barley germplasms under two nitrogen treatments: normal N supply (5.0 mmol L-1) and low N stress (0.1 mmol L-1). Ten agronomic and nitrogen utilization traits were measured under both conditions, and a comprehensive assessment of low-N tolerance was performed using principal component analysis (PCA), nitrogen efficiency indices, and cluster analysis. The results showed that under low-N stress, stalk and leaf fresh weight, root fresh weight, stalk and leaf dry weight, root dry weight, total plant dry weight, N content, and N accumulation significantly decreased, whereas the root-to-shoot ratio, N uptake efficiency, and N utilization efficiency significantly increased. The coefficient of variation (CV) for highland barley traits ranged from 14.01% to 49.80%, with all traits exceeding 10.00% variability. PCA of 12 agronomic traits revealed that the cumulative contribution of the first three principal components reached 91.91% under normal N conditions and 93.13% under low-N conditions. A comprehensive nitrogen efficiency index was developed by integrating trait variability, correlation analysis, and PCA. Based on nitrogen efficiency values and tolerance indices, seven germplasm accessions with strong low-N tolerance were identified: ZDM04507 (Heiqingke), ZDM0468 (Gongjue 1), ZDM04284 (Daxing), ZDM04643 (Nimama), ZDM04480 (Baiqingke), ZDM05597 (Zharen), and ZDM04469 (Huisileng).

Key words: highland barley, low-N tolerance, germplasms screening, comprehensive nitrogen efficiency value, cluster analysis

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