黄河中游粗山羊草三种y-型高分子量谷蛋白亚基的鉴定、克隆及系统进化分析

doi:10.3724/SP.J.1006.2009.01244

Abstract

Abstract:

Aegilops tauschii (2n = 2x = 14, DD), an ancestral specie of Tiriticum aestivum (2n = 6x = 42, AABBDD), possesses extensive allelic variation in high molecular weight glutenin subunits (HMW-GS). The elite genetic recourses are of great value in quality improvement of T. aestivum. Ae. tauschii is distributed in the middle reaches of Yellow River, and its HMW-GS genes have not been investigated in large scales. To detect HMW-GS genes in Ae. tauschii resources in this area and to understand its structure and function by sequencing, 161 Ae. tauschii accessions collected in July 2006 were used in this study with Yumai 49 as a control. Through sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique, three y-type subunits were observed, named as Dy10.5^t, Dy10.4^t, and Dy10.5^*t. Genes of the three subunits were amplified, cloned and sequenced by allele-specific PCR. The results revealed that the deduced amino acid sequence had typical characters of HMW-GS and a general resemblance to each other, but Dy10.4t had a repeated amino acid motif deletion to Dy10.5^t, and Dy10.5^*t had an amino acid substitution sites in signal peptide domain to Dy10.5^t(L-F). From multiple sequence alignment and phylogenetic analysis of storage protein gene family, it is concluded that the subunits Dy10.5^t, Dy10.4^t, and Dy10.5^*t of Ae. tauschii are similar to the Glu-1 locus found in wheat and related species, and are new members of y-type HWM glutenin family.

Key words: Aegilops tauschii, HWM-GS, Molecular cloning, Phylogenetic relationships

SU Ya-Rui,ZHANG Da-Le,ZHANG Ming,LI Suo-Ping*. Characterization,Molecular Cloning and Phylogenetic Analysis of Three y-Type High Molecular Weight Glutenin subunit genes from Aegilops tauschii of the Middle Reaches of Yellow River[J].Acta Agron Sin, 2009, 35(7): 1244-1252.

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Characterization,Molecular Cloning and Phylogenetic Analysis of Three y-Type High Molecular Weight Glutenin subunit genes from Aegilops tauschii of the Middle Reaches of Yellow River

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