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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (4): 580-589.doi: 10.3724/SP.J.1006.2010.00580

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning, Chromosomal Location, and Evolutionary Analysis of α-gliadin Genes from Aegilops tauschii and Common Wheat (Triticum aestivum L.)

ZHU Xi-Ping,LI Xin,LI Ya-Xuan*,YAN Yue-Ming*   

  1. College of Life Science, Capital Normal University,Beijing 100048,China
  • Received:2009-10-19 Revised:2010-01-02 Online:2010-04-12 Published:2010-03-03
  • Contact: LI Ya-Xuan,E-mail:lyx1006@sohu.com;YAN Yue-Ming,E-mail:yanym@hotmail.com

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Abstract:

Seven novel α-gliadin genes from common wheat cultivars (Yumai 34 and Yannong 19) andAegilops tauschii accessions (T9, T197, T48, T176, and T17) were amplified and cloned by using a PCR-based strategy. They were designated as Gli-YM34, Gli-YN19, Gli-T9, Gli-T197, Gli-T48, Gli-T176, and Gli-T17, respectively. Their length of the open reading frame (ORF) ranged from 846 to 891 bp, encoding the putative proteins of 282–297 amino acid residues. Comparative analysis showed that all genes isolated had typical structural characters of α-gliadin genes reported previously. Particularly, Gli-YM34 and Gli-YN19 α-gliadins from common wheat possessed an additional cysteine residue, suggesting a possible positive effect on dough quality. Both genes were assigned to Gli-D2 locus on the chromosome 6D by the analysis of four celiac disease toxic epitopes and glutamine residues in the polyglutamine domain as well as nullisomic-tetrasomic lines of Chinese Spring. A total of twenty-one single nucleotide polymorphisms (SNPs) and a 9 bp deletion among the four typical α-gliadin genes were identified. Phylogenetic and evolutionary analysis revealed that the α-gliadin genes are closely related to LMW-GS genes and their divergence occurred 43.69 million year ago. Less homology was found between α-gliadin genes and HMW-GS and ω-gliadin genes, and they diverged about 79.39 million year.

Key words: α-gliadins, Common wheat, Aegilops tauschii, SNP (Single nucleotide polymorism), Phylogenetics and evolution

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