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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 483-495.doi: 10.3724/SP.J.1006.2017.00483


Linkage Analysis and Genome-Wide Association Study of QTLs Controlling Stem-Breaking-Strength-Related Traits in Wheat

LIU Kai,DENG Zhi-Ying,ZHANG Ying,WANG Fang-Fang,LIU Tong-Tong,LI Qing-Fang,SHAO Wen,ZHAO Bin,TIAN Ji-Chun*,CHEN Jian-Sheng*   

  1. Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China
  • Received:2016-05-27 Revised:2016-11-02 Online:2017-04-12 Published:2016-11-29
  • Contact: Chen Jiansheng, E-mail: jshch@sdau.edu.cn; Tian Jichun, E-mail: jctian@sdau.edu.cn E-mail:liukaiyouxiang@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Shandong Province, China (2015ZRB01179), the breeding project of “Molecular foundation for formation and improvement of main quality traits of wheat”, and the Shandong Provincial Project for Germplasm Resource Innovation.


Stem strengthhas close relationship with lodging character, thereby, affects final yield in wheat. The objectives of this study were to unravel the genetic mechanism of stem-breaking-strength-related traits and find molecular markers closely linked or associated with these traits. We tried to map the stem-breaking-strength-related QTLs through linkage analysis using the RIL population consisting of 173 F8:9 lines derived from Shannong 01-35 ´ Gaocheng 9411) and associationanalysis using a nature population consisting of 2015 wheat varieties. Both populations were planted in two environments and genetically screened with the 90 k SNP array, DArT technology, and traditional molecular markers.By means of the existing high-density genetic map, nine additive QTLs were detectedin different regions onchromosome 4B, such asDURUM_CONTIG63670_287–IACX557 and EX_C101685–RAC875_C27536, which controlled stem-breaking strength, plant height, filling degree of the second internode and culm wall thickness of the second internode and explained 9.40–36.30% of the phenotypic variations.By means of a composite map (containing 24,355 SNPs) based on the IlluminaInfinium assay, a total of 37 SNPs were found in the natural population to be associated withstem-breaking-strength-relatedtraits (P<0.0001). These SNPswere distributed on chromosomes1B, 2B, 2D, 3A, 3B, 4B, 5A, 5D, 6B, 7A, 7B, and 7D and explained 10.70%–36.30% of the phenotypic variations.Thegenetic distance between RAC875_C27536 detected throughlinkage analysis and Tdurum_contig4974_355 detected through genome-wide association analysis was 6.7cM in thecomposite map, indicatingthe presence of importantgenes controlling stem strengthin this region.

Key words: Common wheat, QTL mapping, Linkage analysis, Genome-wide association study (GWAS), Stem-breaking strength

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