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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 829-838.doi: 10.3724/SP.J.1006.2017.00829

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species

DONG Xue1,LIU Meng1,ZHAO Xian-Lin2,*,FENG Yu-Mei1,YANG Yan1,*   

  1. China; 2 Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-huai Region, Ministry of Agriculture, Zhengzhou 450002, China
  • Received:2016-10-02 Revised:2017-01-21 Online:2017-06-12 Published:2017-02-17
  • Contact: 杨燕, E-mail: yangyanchutao@126.com; 赵献林, E-mail: xlin918@126.com E-mail:dongxue225@126.com
  • Supported by:

    1 The functional Laboratory of Plant Biotechnology, College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China; 2 Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-huai Region, Ministry of Agriculture, Zhengzhou 450002, China

Abstract:

The low-molecular-weight glutenin subunits (LMW-GS) account for about one-third of wheat seed storage proteins and have great effects on dough extensibility and food processing quality. Common wheat related species are important genetic resources for wheat improvement. In this study, the specific primers of Glu-A3 genes were used to identify the allelic variations of GluA3-1, GluA-2 and GluA3-3 from nine accessions of Triticum dicoccoides, T. dicoccum, T. durum, and T. boeoricum. A total of 15 new haplotypes were identified, including five in GluA3-1, five in GluA3-2, and five in GluA3-3. Among them, two haplotypes each contained nine cysteines that might be good for improvement of wheat quality. The results about Glu-A3 genes in wheat further made clear composition of LMW-GS and provided a basis for selection of parents in wheat quality breeding.

Key words: Common wheat related species, LMW-GS, Glu-A3 gene, Allelic variation

[1] L?ve á. Conspectus of the Triticeae. Feddes Report, 1984, 95: 425–521 [2] Luo C, Griffin W B, Branlard G, Mcneil D L. Comparison of low- and high molecular-weight wheat glutenin allele effects on flour quality. Theor Appl Genet, 2001, 102: 1088–1098 [3] Zhen S M, Han C X, Ma C Y, Gu A Q, Zhang M, Shen X X, Li X H, Yan Y M. Deletion of the low-molecular-weight glutenin subunit allele Glu-A3a of wheat (Triticum aestivum L.) significantly reduces dough strength and breadmaking quality. BMC Plant Biol, 2014, 14: 1–17 [4] Mcintosh R A,Yamazaki Y, Devos K M, Dubcovsky J, Rogers W J, Appels R. Catalogue of gene symbols for wheat. Proceedings of the 10th international wheat genetics symposium, Paestum, Italy, 2003 [5] Liu C Y, Shepherd K W. Inheritance of B subunits of glutenin and ω-and γ-gliadins in tetraploid wheats. Theor Appl Genet, 1995, 90: 1149–1157 [6] Sreeramulu G, Singh N K. Genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat. Genome, 1997, 40: 41–48 [7] D'Ovidio R, Masci S. The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci, 2004, 39: 321–339 [8] Luo G B, Zhang X F, Zhang Y L, Yang W L, Li Y W, Sun J Z, Zhan K H, Zhang A M, Liu D C. Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biol, 2015, 15: 68 [9] 李黎, 李锁平, 李玉阁. 豫麦50低分子量麦谷蛋白亚基基因的克隆与序列分析. 麦类作物学报, 2015, 35: 1215–1221 Li L, Li S P, Li Y G. Cloning and sequence analysis of LMW-GS genes from common wheat (Triticum aestivum L.) cultivar Yumai 50. J Triticeae Crops, 2015, 35: 1215–1221 (in Chinese with English abstract) [10] Wang Y P, Zhen S M, Luo N N, Han C X, Lu X B, Li X H, Xia X C, He Z H, Yan Y M. Low molecular weight glutenin subunit gene Glu-B3h confers superior dough strength and breadmaking quality in wheat (Triticum aestivum L.). Sci Rep, 2016, 6: 27182 [11] Si H Q, Zhao M L, Zhang X, Yao Guo L, Sun G L, Ma C X. Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.). Sci World J, 2014, 2014: 371045 [12] Wang L H, Zhao X L, He Z H, Ma W, Appels R, Pe?a R J, Xia X C. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor Appl Genet, 2009, 118: 525–539 [13] Zhao X L, Yang Y, He Z H, Lei Z S, Ma W, Sun Q X, Xia X C. Characterization of novel LMW-GS genes at Glu-D3 locus of chromosome 1D in Aegilops tauschii. Hereditas, 2008, 145: 238–250 [14] 程西永, 吴少辉, 李海霞, 董中东, 任妍, 詹克慧, 许海霞. 小麦高、低分子量麦谷蛋白亚基对品质性状的影响. 麦类作物学报, 2014, 34: 482–488 Cheng X Y, Wu S H, Li H X, Dong Z D, Ren Y, Zhan K H, Xu H X. Effects of HMW and LMW glutenin subunits on wheat quality traits. J Triticeae Crops, 2014, 34: 482–488 (in Chinese with English abstract) [15] Ito M, Fushie S, Funatsuki W M, Ikeda T M, Nishio Z, Nagasawa K, Tabiki T, Yamauchi H. Effect of allelic variation in three glutenin loci on dough properties and bread-making qualities of winter wheat. Breed Sci, 2011, 61: 281–287 [16] Jin H, Zhang Y, Li G, Mu P, Fan Z, Xia X, He Z. Effects of allelic variation of HMW-GS and LMW-GS on mixograph properties and Chinese noodle and steamed bread qualities in a set of Aroona near-isogenic wheat lines. J Cereal Sci, 2013, 57: 146–152 [17] Zhang X, Jin H, Zhang Y, Liu D, Li G, Xia X, Zhang A. Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biol, 2011, 12: 1–16 [18] Qin L M, Liang Y, Yang D Z, Sun L, Xia G G, Liu S W. Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution. Dev Genes Evol, 2015, 225: 31–37 [19] Andersen J R, Lübberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8: 554–560 [20] Zhang W, Gianibelli M C, Rampling L R, Gale K R. Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 108: 1409–1419 [21] 孙学永, 马传喜, 司红起, 张琪琪, 乔玉强. 中国小麦微核心种质低分子量麦谷蛋白Glu-A3位点等位基因的PCR检测. 分子植物育种, 2006, 4: 477–482 Sun X Y, Ma C X, Si H Q, Zhang Q Q, Qiao Y Q. Screening Glu-A3 alleles of LMW-GS genes from mini-core collections of Chinese wheat varieties using alleles-specific PCR markers. Mol Plant Breed, 2006, 4: 477–482 (in Chinese with English abstract) [22] 赵曼丽. 低分子量麦谷蛋白亚基基因的克隆及序列分析. 安徽农业大学硕士学位论文, 安徽合肥, 2014 Zhao M L. Cloning and sequence analysis of low molecular weight glutein subunit gene. MS Thesis of Anhui Agricultural University, Hefei, China, 2014 (in Chinese with English abstract) [23] 王林海. 普通小麦及其近缘种低分子量麦谷蛋白基因克隆与STS标记开发. 中国农业科学院博士学位论文, 北京, 2009 Wang L H. Characterisation of Low-Molecular-Weight Glutenin Genes and Development of STS Markers in Common Wheat and Its Relatives. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2009 (in Chinese with English abstract) [24] Gale K R, Ma W, Zhang W, Rampling L, Hill A S, Appels R, Morris P, Morrel M. Simple high-throughput DNA markers for genotyping in wheat. In: Eastwood R, Hollamby G, Rathjen T, Gororo N, eds. Proceedings of 10th Australian Wheat Breeding Assembly. Mildura VIC: Wheat Breeding Society of Australia, 2001. pp 26–31 [25] Stepien L, Holubec V. Chelkowski J. Resistance genes in wild accessions of Triticeae-inoculation test and STS marker analyses. Theor Appl Genet, 2002, 43: 423–435 [26] Qin L M, Liang Y, Yang D Z, Sun L, Xia G M, Liu S W. Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution. Dev Genes Evol, 2015, 225: 1–7 [27] Long H, Wei Y M, Yan Z H, Baum B, Nevo E, Zhen Y L. Classification of wheat low–molecular–weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers. Theor Appl Genet, 2005, 111: 1251–1259 [28] Zhao H X, Wang R J, Guo A G, Hu S W, Sun G L. Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in breed wheat. Hereditas, 2004, 141: 193–198 [29] Xu H, Wang R J, Shen X, Zhao Y L, Sun G L, Zhao H X, Guo A G. Functional properties of a new low -molecular -weight glutenin subunit gene from a bread wheat cultivar. Theor Appl Genet, 2006, 113: 1295–1303

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