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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 829-838.doi: 10.3724/SP.J.1006.2017.00829


Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species

DONG Xue1,LIU Meng1,ZHAO Xian-Lin2,*,FENG Yu-Mei1,YANG Yan1,*   

  1. China; 2 Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-huai Region, Ministry of Agriculture, Zhengzhou 450002, China
  • Received:2016-10-02 Revised:2017-01-21 Online:2017-06-12 Published:2017-02-17
  • Contact: 杨燕, E-mail: yangyanchutao@126.com; 赵献林, E-mail: xlin918@126.com E-mail:dongxue225@126.com
  • Supported by:

    1 The functional Laboratory of Plant Biotechnology, College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China; 2 Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-huai Region, Ministry of Agriculture, Zhengzhou 450002, China


The low-molecular-weight glutenin subunits (LMW-GS) account for about one-third of wheat seed storage proteins and have great effects on dough extensibility and food processing quality. Common wheat related species are important genetic resources for wheat improvement. In this study, the specific primers of Glu-A3 genes were used to identify the allelic variations of GluA3-1, GluA-2 and GluA3-3 from nine accessions of Triticum dicoccoides, T. dicoccum, T. durum, and T. boeoricum. A total of 15 new haplotypes were identified, including five in GluA3-1, five in GluA3-2, and five in GluA3-3. Among them, two haplotypes each contained nine cysteines that might be good for improvement of wheat quality. The results about Glu-A3 genes in wheat further made clear composition of LMW-GS and provided a basis for selection of parents in wheat quality breeding.

Key words: Common wheat related species, LMW-GS, Glu-A3 gene, Allelic variation

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