基于元分析的大豆生育期QTL的整合

doi:10.3724/SP.J.1006.2009.01418

Abstract

Abstract:

Soybean is one of the most important crops in the world, which is kept improving for its yield and quality. Growth stage is a critical trait in soybean development and production which is one of the quantitive traits depending on many loci. As far as the technology of QTL comes, quantitive traits mapping has becoming hot point. Located the QTL controlling soybean growth stage by genetic linkage, is very useful to molecular breeding and deeper understand the process of growth stage develop. Till now, a lot of QTLs related with soybean growth stage were mapped, but many pseudo-positive QTLs were included. To mining the true and major QTLs, meta-analysis were introduced in this study. According to the map of soybean soymap2 published in 2004, an integrated QTL map of soybean growth stages was constructed. The QTLs of soybean growth stage were collected in recent 12 years, and projected to the reference map from their own maps by the software BioMercator2.1. In total, 98 QTLs related with different growth stage of soybean were integrated, including the QTLs of vegetative growth and reproductive growth. A method of meta-analysis was used to narrow down the confidence interval. Seven R1 real QTLs and two R8 real QTLs as well as their corresponding markers were obtained respectively, and a known gene was found in a mapping interval in LG L, located on 93.26 cM. The shortest confidence interval is only 0.9 cM in LG C2, with the marker A397_1 on the left and the marker Satt263 on the right. And 10 QTLs in 5 linkage groups, including C2, D1a, D1b, F, and J, were related to several growth stages. In the combined analysis, a QTL on 55.89 cM in LG D1a controls 6 growth stages, which were R1, R2, R3, R4, R5, and R7. Another QTL on LG D1b, near the markers Bng047_1 and Sat_169, were partly related not only in vegetative growth but also in reproductive growth. The results offer a basis for gene mining and molecular breeding in soybean.

Key words: Soybean, Growth stage, Meta-Analysis, Real QTL, QTL projection

WU Qiong,QI Zhao-Ming,LIU Chun-Yan,HU Guo-Hua,CHEN Qing-Shan. An Integrated QTL Map of Growth Stage in Soybean[Glycine max(L.) Merr.]: Constructed through Meta-Analysis[J].Acta Agron Sin, 2009, 35(8): 1418-1424.

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An Integrated QTL Map of Growth Stage in Soybean[Glycine max(L.) Merr.]: Constructed through Meta-Analysis

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