Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (1): 85-91.doi: 10.3724/SP.J.1006.2010.00085

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Characterization of Two Fiber Elongation Genes Using a Cotton Fiber Developmental Mutant (Gossypium hirsutum L.)

WANG Lei,ZHU Yi-Chao**,CAI Cai-Ping,ZHANG Tian-Zhen,GUO Wang-Zhen*   

  1. National Key Laboratory of Crop Genetics and Germplasm enhancement,Cotton Research Institute,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2009-04-15 Revised:2009-06-25 Online:2010-01-12 Published:2009-11-17
  • Contact: GUO Wang-Zhen, E-mail: moelab@njau.edu.cn; Tel: 025-84395311

PDF

1169

Abstract:

Cotton fibers are single-celled seed trichomes of major economic importance. Many important genes are expressed during cotton fiber development and fiber developmental mutants can be used to preferentially detect the genes controlling fiber development. The Ligon lintless mutant (Li1li1) is a fiber elongation developmental mutant with a dominant monogenetic mutation characterized by short fibers and distorted leaf, stem and flower growth, and the recessive pure line (li1li1) exhibits normal fiber developmental characteristics. The objectives of this study were to isolate genes preferentially or specifically expressed in fiber elongation stage by comparing gene expression differences between Li1li1 and li1li1. RNA isolated from 10 days post anthesis (DPA) fibers and ovules mixture in Li1li1 and li1li1 were used to screen differential gene expression in fiber development using differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Two differential expression cDNA segments were isolated, the corresponding full-length cDNAs were cloned and their primary function was analyzed. The two genes encoded 542 and 667 amino acid residues and functioned as glutamate decarboxylase (GhGAD) and vacuole-pyrophosphatase (GhVP1), respectively. Transcriptional level assays showed the two genes were constitutively expressed in tested tissues with higher expression levels during the fiber elongation stage. Further, a BC1 mapping population derived from hybridization between G. hirsutum acc. TM-1 and G. barbadense cv. Hai 7124, and TM-1 as the recurrent parent, was used for the location of GhGAD and GhVP1 on chromosomes 12 and 8, respectively, using cleaved amplified polymorphic sequences (CAPs).

Key words: Cotton, Fiber developmental mutant, DDRT-PCR, Glutamate decarboxylase(GhGAD), Vacuole-pyrophosphatase(GhVP1)

[1] Fryxell P A. The Natural History of the Cotton Tribe. College Station, TX: Texas A&M University Press, 1979
[2] Basra A S, Malik C P. Development of cotton fiber. Inter Rev Cytol, 1984, 89: 65-113
[3] Orford S J, Timmis J N. Abundant mRNAs specific to the developing cotton fiber. Theor Appl Genet, 1997, 94: 909-918
[4] Turley R B, Ferguson D L. Changes of ovule proteins during early fiber developing in a normal and a fiberless line of cotton (Gossypium hirsutum L.). J Plant Physiol, 1996, 149: 695-702
[5] Dhindsa R S, Beasley R B, Ting I P. Osmoregulation in cotton fiber: Accumulation of potassium and malate during growth. Plant Physiol, 1975, 56: 394-398
[6] Basra A S, Malik C P. Dark metabolism of CO2 during elongation of two cottons differing in fiber lengths. J Exp Bot, 1983, 34: 1-9
[7] John M E, Crow L J. Gene expression in cotton (Gossypium hirsutum L.) fiber: Cloning of the mRNAs. Proc Natl Acad Sci USA, 1992, 89: 5769-5773
[8] Kohel R J. Linkage tests in upland cotton, Gossypium hirsutum L. Crop Sci, 1972, 12: 66-69

[9] Karaca M, Saha S, Jenkins J N, Zipf A, Kohel R, Stelly D M. Simple sequence repeat (SSR) markers linked to the Ligon Lintless (Li1) mutant in cotton. J Hered, 2002, 93: 221-224
[10] Wan C Y, Wilkins T A. A modified hot borate method significantly enhances the yield of high quality RNA from cotton (Gossypium hirsutum L.). Anal Biochem, 1994, 223: 7212
[11] Liang P, Pardee A B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 1992, 257: 967-971
[12] Winer J, Jung C K, Shackel I, Williams P M. Development and validation of real-time quantitative reverse transcriptase polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem, 1999, 270: 41-49
[13] Han Z G, Guo W Z, Song X L. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboretum in allotetraploid cotton. Mol Genet Genom, 2004, 272: 308-327
[14] Guo W, Cai C, Wang C, Zhao L, Wang L, Zhang T. A preliminary analysis of genome structure and composition in Gossypium hirsutum. BMC Genom, 2008, 9: 314
[15] Van-Ooijen J W, Voorrips R E. JoinMapR Version 3.0: Software for the Calculation of Genetic Linkage Maps, CPRO-DLO, Wageningen, 2001
[16] Liu R H(刘仁虎), Meng J L(孟金陵). MapDraw: A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (遗传), 2003, 25(3): 317-321 (in Chinese with English abstract)
[17] Roberts E, Frankel S. Gamma-Aminobutyric acid in brain: Its formation from glutamic acid. J Biol Chem, 1950, 187: 55
[18] Satyanarayan V, Nair P M. Enzymolgy and possible roles of 4-aminobutyrate in higher plants. Phytochem, 1990, 29: 367-375
[19] Baum G, Chen Y, Arazi T, Takatsuji H, Fromm H. A plant glutamate decarboxylase containing a calmodulin binding domain.J Biol Chem, 1993, 268: 19610-19617
[20] Rea P A. Vacuolar H+-translocating pyrophosphatases: a new category of ion translocase. Trends Biochem Sci, 1992, 17: 348-353
[21] Rea P A, Poole R J. Vacuolar H+-translocating pyrophosphatase. Plant Mol Biol, 1993, 44: 157-180
[22] Zhen R G, Kim E J, Rea P A.The molecular and biochemical basis of pyrophosphate-energized proton translocation at the vacuolar membrane. Adv Bot Res, 1997,25: 297-337
[23] Maeshima M. Vacuolar H+-pyrophosphatase. Biochim Biophys Acta, 2000, 1465: 37-51
[24] Smart L B, Vojdani F, Maeshima M, Wilkins T A. Genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated. Plant Physiol, 1998, 116: 1539-1549
Roberto A G, Jisheng L, Soledad U, Lien M D, Gethyn J A, Seth L A, Gerald R F. Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc Natl Acad Sci USA, 2001, 98: 11444-11449
[1] ZHOU Jing-Yuan, KONG Xiang-Qiang, ZHANG Yan-Jun, LI Xue-Yuan, ZHANG Dong-Mei, DONG He-Zhong. Mechanism and technology of stand establishment improvements through regulating the apical hook formation and hypocotyl growth during seed germination and emergence in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1051-1058.
[2] SUN Si-Min, HAN Bei, CHEN Lin, SUN Wei-Nan, ZHANG Xian-Long, YANG Xi-Yan. Root system architecture analysis and genome-wide association study of root system architecture related traits in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1081-1090.
[3] YAN Xiao-Yu, GUO Wen-Jun, QIN Du-Lin, WANG Shuang-Lei, NIE Jun-Jun, ZHAO Na, QI Jie, SONG Xian-Liang, MAO Li-Li, SUN Xue-Zhen. Effects of cotton stubble return and subsoiling on dry matter accumulation, nutrient uptake, and yield of cotton in coastal saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(5): 1235-1247.
[4] ZHENG Shu-Feng, LIU Xiao-Ling, WANG Wei, XU Dao-Qing, KAN Hua-Chun, CHEN Min, LI Shu-Ying. On the green and light-simplified and mechanized cultivation of cotton in a cotton-based double cropping system [J]. Acta Agronomica Sinica, 2022, 48(3): 541-552.
[5] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[6] ZHANG Te, WANG Mi-Feng, ZHAO Qiang. Effects of DPC and nitrogen fertilizer through drip irrigation on growth and yield in cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 396-409.
[7] ER Chen, LIN Tao, XIA Wen, ZHANG Hao, XU Gao-Yu, TANG Qiu-Xiang. Coupling effects of irrigation and nitrogen levels on yield, water distribution and nitrate nitrogen residue of machine-harvested cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 497-510.
[8] ZHAO Wen-Qing, XU Wen-Zheng, YANG Liu-Yan, LIU Yu, ZHOU Zhi-Guo, WANG You-Hua. Different response of cotton leaves to heat stress is closely related to the night starch degradation [J]. Acta Agronomica Sinica, 2021, 47(9): 1680-1689.
[9] YUE Dan-Dan, HAN Bei, Abid Ullah, ZHANG Xian-Long, YANG Xi-Yan. Fungi diversity analysis of rhizosphere under drought conditions in cotton [J]. Acta Agronomica Sinica, 2021, 47(9): 1806-1815.
[10] ZENG Zi-Jun, ZENG Yu, YAN Lei, CHENG Jin, JIANG Cun-Cang. Effects of boron deficiency/toxicity on the growth and proline metabolism of cotton seedlings [J]. Acta Agronomica Sinica, 2021, 47(8): 1616-1623.
[11] GAO Lu, XU Wen-Liang. GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development [J]. Acta Agronomica Sinica, 2021, 47(7): 1239-1247.
[12] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[13] XU Nai-Yin, ZHAO Su-Qin, ZHANG Fang, FU Xiao-Qiong, YANG Xiao-Ni, QIAO Yin-Tao, SUN Shi-Xian. Retrospective evaluation of cotton varieties nationally registered for the Northwest Inland cotton growing regions based on GYT biplot analysis [J]. Acta Agronomica Sinica, 2021, 47(4): 660-671.
[14] ZHOU Guan-Tong, LEI Jian-Feng, DAI Pei-Hong, LIU Chao, LI Yue, LIU Xiao-Dong. Efficient screening system of effective sgRNA for cotton CRISPR/Cas9 gene editing [J]. Acta Agronomica Sinica, 2021, 47(3): 427-437.
[15] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd