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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2091-2098.doi: 10.3724/SP.J.1006.2010.02091

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of a CBS Domain Containing Protein Gene TaCDCP1 from Wheat

WANG Xiao-Min1,FENG Hao1,SUN Yan-Fei1,LIU Bo1,WANG Xiao-Jie1,XU Liang-Sheng1,YU Xiu-Mei1,WEI Guo-Rong1,HUANG Li-Li1,KANG Zhen-Sheng1,2,*   

  1. 1 College of Plant Protection, Northwest A&F University, Yangling 712100, China; 2 Shaanxi Provincial Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China
  • Received:2010-05-24 Revised:2010-08-03 Online:2010-12-12 Published:2010-10-22
  • Contact: 康振生,E-mail:kangzs@nwsuaf.edu.cn

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Abstract: To elucidate the defense response of wheat(Triticum aestivum L.) to Puccinia striiformis f. sp. tritici (Pst), we constructed the incompatible interaction SSH cDNA library of wheat(cv. Suwon 11) leaves infected by Pst CYR23. A total of 652 unigenes were identified and 424 genes were annotated. On the basis of previous study, according to cDNA sequence LWSRP2502 (Genbank accession No. EV254338), a full-length sequence of the CBS domain containing protein gene, tentatively designated as TaCDCP1 (Triticum aestivum CBS domain containing protein 1), was isolated and characterized from wheat leaves infected by Pst through in silico cloning and reverse transcription PCR (RT-PCR) approaches.The open reading frame of TaCDCP1 was 654 bp in length andpredicted to encode 217 amino acids protein which contained two conserved cystathionine beta-synthase (CBS) domains and was without transmembrane domain or signal peptide sequence. The deduced protein was predicted existing in chloroplast stroma. The amino acid sequence of TaCDCP1 shares 92%, 72%, and 63% identify withthe homologs in barley (Hordeum vulgare) , rice (Oryza sativa) and maize (Zea mays), respectively. The TaCDCP1 gene was highly expressed in leaves than in roots and stems. Challenged by Pst, TaCDCP1 was induced by this fungus in both incompatible and compatible interactions, with the maximal expression at 18 h post inoculation (hpi) and 24 hpi, respectively. Its transcript accumulation was much higher in the incompatible interaction than in the compatible interaction at the early stage of infection (18–48 hpi), but much lower at the late stage (96–120 hpi). The expression of TaCDCP1 wasalso up-regulated after treated by phytohormones such as abscisic acid (ABA), and down-regulated by benzyladenine, ethylene, gibberellins, methyl jasmonate and salicylic acid to a certain degree. And it was obviously up-regulated by various abiotic stresses, such as low temperature and drought. However, mechanical wound and high salinity stress could not induce the expression of TaCDCP1. These results suggest that TaCDCP1 is probably involved in the disease resistance and defense response in wheat to Pst through ABA and ethylene pathways, and also participate in the signal transmission pathways under low temperature, and drought conditions.

Key words: Wheat, Stripe rust fungus, CBS domain, Abiotic stresses, Gene expression

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