Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2091-2098.doi: 10.3724/SP.J.1006.2010.02091


Cloning and Expression Analysis of a CBS Domain Containing Protein Gene TaCDCP1 from Wheat

WANG Xiao-Min1,FENG Hao1,SUN Yan-Fei1,LIU Bo1,WANG Xiao-Jie1,XU Liang-Sheng1,YU Xiu-Mei1,WEI Guo-Rong1,HUANG Li-Li1,KANG Zhen-Sheng1,2,*   

  1. 1 College of Plant Protection, Northwest A&F University, Yangling 712100, China; 2 Shaanxi Provincial Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China
  • Received:2010-05-24 Revised:2010-08-03 Online:2010-12-12 Published:2010-10-22
  • Contact: 康振生,E-mail:kangzs@nwsuaf.edu.cn

Abstract: To elucidate the defense response of wheat(Triticum aestivum L.) to Puccinia striiformis f. sp. tritici (Pst), we constructed the incompatible interaction SSH cDNA library of wheat(cv. Suwon 11) leaves infected by Pst CYR23. A total of 652 unigenes were identified and 424 genes were annotated. On the basis of previous study, according to cDNA sequence LWSRP2502 (Genbank accession No. EV254338), a full-length sequence of the CBS domain containing protein gene, tentatively designated as TaCDCP1 (Triticum aestivum CBS domain containing protein 1), was isolated and characterized from wheat leaves infected by Pst through in silico cloning and reverse transcription PCR (RT-PCR) approaches.The open reading frame of TaCDCP1 was 654 bp in length andpredicted to encode 217 amino acids protein which contained two conserved cystathionine beta-synthase (CBS) domains and was without transmembrane domain or signal peptide sequence. The deduced protein was predicted existing in chloroplast stroma. The amino acid sequence of TaCDCP1 shares 92%, 72%, and 63% identify withthe homologs in barley (Hordeum vulgare) , rice (Oryza sativa) and maize (Zea mays), respectively. The TaCDCP1 gene was highly expressed in leaves than in roots and stems. Challenged by Pst, TaCDCP1 was induced by this fungus in both incompatible and compatible interactions, with the maximal expression at 18 h post inoculation (hpi) and 24 hpi, respectively. Its transcript accumulation was much higher in the incompatible interaction than in the compatible interaction at the early stage of infection (18–48 hpi), but much lower at the late stage (96–120 hpi). The expression of TaCDCP1 wasalso up-regulated after treated by phytohormones such as abscisic acid (ABA), and down-regulated by benzyladenine, ethylene, gibberellins, methyl jasmonate and salicylic acid to a certain degree. And it was obviously up-regulated by various abiotic stresses, such as low temperature and drought. However, mechanical wound and high salinity stress could not induce the expression of TaCDCP1. These results suggest that TaCDCP1 is probably involved in the disease resistance and defense response in wheat to Pst through ABA and ethylene pathways, and also participate in the signal transmission pathways under low temperature, and drought conditions.

Key words: Wheat, Stripe rust fungus, CBS domain, Abiotic stresses, Gene expression

[1]Chen X M, Line R F. Inheritance of stripe rust (yellow rust) resistance in the wheat cultivar Carstens V. Euphytica, 1993, 71: 107–113
[2]Chen X M. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Can J Plant Pathol, 2005, 27: 314–337
[3]Li Z-Q(李振岐), Zeng S-M(曾士迈). Stripe Rust in China (中国小麦锈病). Beijing: China Agriculture Press, 2002. pp 2–3 (in Chinese)
[4]Bateman A. The structure of a domain common to archaebacteria and the homocystinuria disease protein. Trends Biochem Sci, 1997, 22: 12–13
[5]Hemant R K, Anil K S, Sudhir K S, Sneh L S P, Ashwani P. Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation. BMC Genomics, 2009, 10: 200
[6]Woods A, Cheung P C F, Smith F C, Davison M D, Scott J, Beri R K, Carling D. Characterization of AMP-activated protein kinase and subunits. J Biol Chem, 1996, 271: 10282–10290
[7]Sintchak M D, Fleming M A, Futer O, Raybuck S A, Chambers S P, Caron P R, Murcko M A, Wilson K P. Structure and mechanism of inosine monophosphate dehydrogenase in complex with the immunosuppressant mycophenolic acid. Cell, 1996, 85: 921–930
[8]Schmidt-Rose T, Jentsch T J. Reconstitution of functional voltage-gated chloride channels from complementary fragments of CLC-1. J Biol Chem, 1997, 272: 20515–20521
[9]Shan X Y, Kruger W D. Correction of disease-causing CBS mutations in yeast. Nat Genet, 1998, 19: 91–93
[10]Ignoul S, Eggermont J. CBS domains: structure, function, and pathology in human proteins. Am J Physiol-Cell Physiol, 2005, 289: 1369–1378
[11]Wang X L, Ren X, Zhu L L, He G C. OsBi1, a rice gene, encodes a novel protein with a CBS-like domain and its expression is induced in responses to herbivore feeding. Plant Sci, 2004, 166: 1581–1588
[12]Manickavelu A, Kawaura K, Oishi K, Shin-I T, Kohara Y, Yahiaoui N, Keller B, Suzuki A, Yano K, Ogihara Y. Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by Puccinia triticina. DNA Res, 2010, DOI: 10.1093/dnares/dsq009
[13]Yu X-M(于秀梅). Construction of SSH cDNA library of Wheat Leaves Induced by Puccinia striiformis and Its ESTs Analysis. PhD Dissertation of Northwest A&F University, 2006. pp 62–66 (in Chinese with English abstract)
[14]Kang Z-S(康振生), Li Z-Q(李振岐). Discovery of pathogenic isolates of stripe rust on cultivar Lovrin 10 at normal temperature. J Northwest Agric Coll (西北农林科技大学学报), 1984, 12(4): 18–28 (in Chinese with English abstract)
[15]Zhang H B, Zhang D B, Chen J, Yang Y H, Huang Z J, Huang D F, Wang X C, Huang R F. Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum. Plant Mol Biol, 2004, 55: 825–834
[16]Sato K, Shin-I T, Seki M, Shinozaki K, Yoshida H, Takeda K, Yamazaki Y, Conte M, Kohara Y. Development of 5006 full-length cDNAs in barley: a tool for accessing cereal genomics resources. DNA Res, 2009, 16: 81–89
[17]Alexandrov N N, Brover V V, Freidin S, Troukhan M E, Tatarinova T V, Zhang H, Swaller T J, Lu Y P, Bouck J, Flavell R B. Insights into corn genes derived from large-scale cDNA sequencing. Plant Mol Biol, 2009, 69: 179–194
[18]Yu X M, Yu X D, Qu Z P, Huang X J, Guo J, Han Q M, Zhao J, Huang L, Kang Z S. Cloning of a putative hypersensitive induced reaction gene from wheat infected by stripe rust fungus. Gene, 2008, 407: 193–198
[19]Yu G X, Braun E, Wise R P. Rds and Rih mediate hypersensitive cell death independent of gene-for-gene resistance to the oat crown rust pathogen Puccinia coronata f. sp. avenae. Mol Plant Microbe Interact, 2001, 14: 1376–1383
[20]Hand J-D(韩建东), Cao Y-Y(曹远银), Yao P(姚平). Hypersensitive response and activity dynamics of defense enzymes induced by elicitor(s) from wheat-stem rust interaction systerm. Acta Agr Boreali-Sinica (华北农学报), 2009, 24(1): 79–82 (in Chinese with English abstract)
[21]Wang C F, Huang L L, Buchenauer H, Han Q M, Zhang H C, Kang Z S. Histochemical studies on the accumulation of reactive oxygen species (O2- and H2O2) in the incompatible and compatible interaction of wheat-Puccinia striiformis f. sp. tritici. Physiol Mol Plant Pathol, 2007, 71: 230–239
[22]Kang Z S, Huang L L, Buchenauer H. Ultrastructural changes and localization of lignin and callose in compatible and incompatible interactions between wheat and Puccinia striiformis. J Plant Dis Protect, 2002, 109: 25–37
[23]Zhu Q, Dröge-Laser W, Dixon R A, Lamb C. Transcriptional activation of plant defense genes. Curr Opin Genet Dev, 1996, 6: 624–630
[24]Glazebrook J. Genes controlling expression of defense responses in Arabidopsis. Curr Opin Plant Biol, 1999, 2: 280–286
[25]Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol, 2005, 43: 205–227
[26]Koornneef A, Pieterse C M J. Crosstalk in defense signaling. Plant Physiol, 2008, 146: 839–844
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[3] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[4] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[5] WU Yan-Fei, HU Qin, ZHOU Qi, DU Xue-Zhu, SHENG Feng. Genome-wide identification and expression analysis of Elongator complex family genes in response to abiotic stresses in rice [J]. Acta Agronomica Sinica, 2022, 48(3): 644-655.
[6] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[7] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[8] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[9] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[10] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[11] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[12] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[13] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[14] QU Jian-Zhou, FENG Wen-Hao, ZHANG Xing-Hua, XU Shu-Tu, XUE Ji-Quan. Dissecting the genetic architecture of maize kernel size based on genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(2): 304-319.
[15] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
Full text



No Suggested Reading articles found!