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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 327-334.doi: 10.3724/SP.J.1006.2010.00327


Effect of Inorganic Nitrogen Forms on Growth and Kinetics of Ammonium and Nitrate Uptake in Camellia sinensis L.

DU Xu-Hua1,2,PENG Fang-Ren1,*   

  1. 1 College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2 China National Bamboo Research Center, State Forestry Administration, Hangzhou 310012, China
  • Received:2009-06-08 Revised:2009-10-04 Online:2010-02-10 Published:2009-12-21
  • Contact: PENG Fang-Ren, E-mail: frpeng@njfu.com.cn E-mail:stary8@163.com


The ammonium and nitrate nitrogen are the main inorganic nitrogen in crop cultivation, the rational application of chemical N fertilizer and improvement of N use efficiency in tea plant are important for tea cultivation. There was little research on the relationship between inorganic nitrogen form and root absorption properties of perennial woody plants. In this study, four tea plant cultivars (Wuniuzao, Longjing 43, Yingshuang, and Zhenong 139) were selected for a pot experiment, and the influences of different N fertilizers with different ratio of NO3--N : NH4+-N on height growth and girth growth of tea were studied. In order to explore the absorption mechanism of different inorganic N forms, Michaelis-Menten equations of different N forms were established and compared. The results showed that the chemical nitrogen form of the fertilizer had a very significant effect on the height growth of the tea, but no significant effect on girth growth. The tea height significantly increased, and the increment amount reached 15.45 cm per year at 5:5 of NO3--N : NH4+-N ratio, while at the pure NH4+-N treatment, the increment amount was only 7.03. Compared with other tea cultivars, the increasing amount in height of Wuniuzao was the most, and in girth of Longjing 43 was the least. The maximum uptake rate (Vmax) for nitrate nitrogen (NO3--N) absorption decreased when the proportion of ammonium nitrogen (NH4+-N) increased. The affinity to NO3--N was enhanced gradually, but weakened when the proportion of NH4+-N was over 50%. The Vmax for NH4+-N absorption increased quickly until the proportion of NH4+-N was over 50%, and then decreased slowly. The affinity to NH4+-N weakened slowly until the NH4+-N proportion was over 50%, and quickly when the ammonium proportion was too high. When the NO3-: NH4+ was 5:5, the Vmax values for NO3-Nand NH4+-N were 0.24–0.35 and 0.19–0.30 mmol FW g-1 d-1, respectively, and the Km values were 0.65–0.74 and 0.63–0.80 mmol L-1, respectively, indicating that the nitrogen absorbing status was better than that of other treatments. In the pot experiment, nitrogen forms had a great effect on the nitrogen absorption kinetics, indicating the tea tree had an adaptation mechanism to the change of N fertilizer.

Key words: Camellia sinensis L., Nitrogen form, Seedling height growth, Girth growth, Kinetics of inorganic nitrogen absorption

[1] Duan Y-H(段英华), Zhang Y-L(张亚丽), Shen Q-R(沈其荣). Effect of nitrate on the ammonium uptake and growth of different genotypes of rice (Oryza sativa L.) at the seedling stage. Acta Pedolog Sin (土壤学报), 2005, 42(12): 260-265 (in Chinese with English abstract)

[2] Feng K(封克), Wang X-L(汪晓丽), Chen P(陈平), Sheng H-J(盛海君). Nitrate uptake of rice and the effect of ammonium on it at diferent growth stages. Sci Agric Sin (中国农业科学), 2003, 36(3): 307-312 (in Chinese with English abstract)

[3] Yang X-E(杨肖娥), Sun X(孙羲). A kinetic study on NH4+ and NO3- uptake by various rice varieties. Chin J Soil Sci (土壤通报), 1991, 22(5): 222-224 (in Chinese)

[4] He W-S(何文寿), Li S-X(李生秀), Li H-T(李辉桃). Characteristics of absorbing ammonium and nitrate nitrogen of six crops at different growth stages. Acta Agron Sin (作物学报), 1999, 25(2): 221-226 (in Chinese with English abstract)

[5] Tian X-H(田霄鸿), Li S-X(李生秀), Wang Q-J(王清君). Uptake capacity of ammonium and nitrate to several crops. Chin J Soil Sci (土壤通报), 2001, 32(1): 16-18 (in Chinese)

[6] Zhang Y-D(张彦东), Fan Z-Q(范志强), Wang Q-C(王庆成), Wang Z-Q(王政权). Effect of different nitrogen forms on growth of Fraxinus mandshurica seedlings. Chin J Appl Ecol (应用生态学报), 2000, 11(5): 665-667 (in Chinese with English abstract)

[7] Chen Y-L(陈永亮), Liu M-H(刘明河), Li X-L(李修岭). Effects of different nitrogen forms and ratios on the photosynthetic characteristics of Pinus koraiensis seedlings. J Nanjing For Univ (Nat Sci Edn)(南京林业大学学报·自然科学版), 2005, 29(3): 77-80 (in Chinese with English abstract)

[8] Cruz C, Lips S H, Martins-Loucao M A. The effect of nitrogen on photosynthesis of carbon at high CO2 concentrations. Physiol Plant, 1993, 89: 552-556

[9] Xiao K(肖凯), Zhang S-H(张树华), Zou D-H(邹定辉), Zhang R-X(张荣铣). The effects of different nitrogen nutrition forms on photosynthetic characteristics in wheat leaves. Acta Agron Sin (作物学报), 2000, 26(1): 53-58 (in Chinese with English abstract)

[10]Cao C-L(曹翠玲), Li S-X(李生秀). Effects of N form on some physiological characteristics and yield of wheat during the vegetative and reproductive growth stage. Acta Agron Sin (作物学报), 2003, 29(2): 258-262 (in Chinese with English abstract)

[11]Sun C-F(孙传范), Dai T-B(戴廷波), Cao W-X(曹卫星). Effect of the enhanced ammonium nutrition on the growth and nitrogen utilization of wheat under different N levels. Plant Nutr Fert Sci (植物营养与肥料学报), 2003, 9(1): 33-38 (in Chinese with English abstract)

[12]Zhang X-Y(张新要), Liu W-Q(刘卫群), Yi J-H(易建华), Li T-F(李天福), Chen L-C(陈良存), Zhou W-H(周文辉). Effects of different forms of nitrogen fertilizer on predominant enzymes of C and N metabolism in red and rice soils of flue-cured tobacco. J Yunnan Agric Univ (云南农业大学学报), 2005, 20(2): 225-230 (in Chinese with English abstract)

[13]Engels C, Marschner H. Influence of the form nitrogen supply on root uptake and translocation of cations in the xylem exudates of maize. J Exp Bot,1993, 44: 1695-1701

[14]Ruan T Y, Zhang F S, Mink H W. Effect of nitrogen form and phosphorus source on the growth, nutrient uptake and rhizosphere soil properth of Camellia sinenisis L. Plant Soil, 2000, 223: 63-71

[15]Ma X-M(马新明), Wang Z-Q(王志强), Wang X-C(王小纯), Wang S-L(王书丽). Effects of nitrogen forms on roots and N fertilizer efficiency of different wheat cultivars with specialized end-uses. Chin J Appl Ecol (应用生态学报), 2004, 15(4): 655-658 (in Chinese with English abstract)

[16]Guo P-G(郭培国), Chen J-J(陈建军), Zheng Y-L(郑燕玲). Study on the effects of nitrogen forms on photosynthetic characteristics in flue-cured tobacco. Chin Bull Bot (植物学通报), 1999, 16(3): 262-267 (in Chinese with English abstract)

[17]Wang X B, Wu P, Hu B, Chen Q S. Effects of nitrate on the growth of lateral root and nitrogen absorption in rice. Acta Bot Sin, 2002, 44: 678-683

[18]Zhao J-R(赵建荣), Fan W-G(樊卫国). Effects of nitrogen forms on the pH value of culture medium and root growth of Pyrus. Pashia Buch-Ham. J Mountain Agric Biol (山地农业生物学报), 2005, 24(2): 128-130 (in Chinese with English abstract)

[19]Zhang H, Jennings A, Barlow P W, Forde B G. Dual pahtways for regulation of root branching by nitrate. Plant Biol, 1999, 96: 6529-6534

[20]Aslam M, Travis R, Huffaker R. Comparative kinetics and reciprocal inhibition of nitrate and nitrite uptake in roots of uninduced and induced barley (Hordeum vulgare L.) seedlings. Plant Physiol, 1992, 99: 1124-1133

[21]Glass A D M, Shaff J E, Kochian L V. Studies of the uptake of nitrate in barley. Ⅳ: Electrophysiology. Plant Physiol, 1992, 99: 456-463

[22]Munos S, Cazettes C, Fizames C, Gaymard F,Tillard P, Lepetit M, Lejay L, Gojon A. Transcript profiling in the chl1-5mutant of Abrabidopsis reveals a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1. Plant Cell, 2004, 16: 2433-2447

[23]Han Y-L(韩燕来), Xu F-S(徐芳森), Duan H-Y(段海燕), Shi L(石磊), Wang Y-H(王运华). Advances in study on proteins which transport nutrients in Arabidopsis thaliana. Chin Bull Bot (植物学通报), 2003, 20(1): 23-30 (in Chinese with English abstract)

[24]Duan Y-H(段英华), Zhang Y-L(张亚丽), Shen Q-R(沈其荣), Chen H-Y(陈红云), Zhang Y(张勇). Effect of partial replacement of NH4+ by NO3- on nitrogen uptake and utilization by different genotypes of rice at the seedling stage. Plant Nutr Fert Sci (植物营养与肥料学报), 2005, 11(2): 160-165(in Chinese with English abstract)

[25]Wang X-L(汪晓丽), Feng K(封克), Sheng H-J(盛海君), Chen P(陈平). Kinetics of nitrate uptake by different rice genotypes and the effects of ammonium on nitrate uptake at the seedling stage. Sci Agric Sin (中国农业科学), 2003, 36(11): 1306-1311 (in Chinese with English abstract)

[26]Zhou X-H(周晓红), Wang G-X(王国祥), Yang F(杨飞), He W(何伟), Yang Z(杨周). Uptake kinetic characteristics of different ammonium and nitrate by Ipomoea aquatica Forsk. Res Soil Water Conserv (水土保持研究), 2008, 15(5): 84-86 (in Chinese with English abstract)

[27]Zhao Y(赵越), Ma F-M(马凤鸣), Zhang D-Y(张多英). Study on the absorption kinetics of different nitrogen in sugar beet. J Northeast Agric Univ (东北农业大学学报), 2006, 37(3): 294-298 (in Chinese with English abstract)

[28]Forde B G, Clarkson D T. Nitrate and ammonium nutrition of plants: Physiological molecular perspectives. Adv Botl Res, 1999, 30: 1-90

[29]Kronzucker H J, Britto D T, Davenport R J, Tester M A. Ammonium toxicity and the real cost of transport. Trends Plant Sci, 2001, 6: 335-337

[30]Crawford N M, Glass A D M. Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci, 1998, 3: 389-395

[31]Schubert S, Yan Y. Nitrate and ammonium nutration of plants: Effects on acid/base balance and adaptation of root cell plasmalemma H+ ATPase. Zeit schrift fur Pflanzenernahrung und Bodenkunde, 1996, 160: 275-281

[32]Colmer T D, Bloom A J. A comparison of NH4+ and NO3- net fluxes along roots of rice and maize. Plant Cell Environ, 1998, 21: 240-246

[33]Sitt M, Scheible W R. Nitrate acts as a signal to control gene expression, metabolism and biomass allocation. In: Kruger N, Hill S A, Ratcliffe R G, eds. Regulation of primary metabolic pathways in plants. the Netherlands: Kluwer Academic Publishers, 1999. pp 275-306

[34]Takei K, Takahashi T, Sugiyama T, Yamaya T, Sakakibara H. Multiple routes communicating nitrogen availability from roots to shoots: A signal transduction pathway mediated by cytokinin. J Exp Bot, 2000, 53: 971-977

[35]Forde B G. Local and long-range signaling pathways regulating plant responses to nitrate. Annu Rev Plan Biol, 2002, 53: 203-224

[36]Wang X B, Wu P, Xia M, Wu Z C, Chen Q S, Liu F Y. Identification of genes enriched in rice roots of the local nitrate treatment and their expression patterns in split-root treatment. Gene, 2002, 297: 93-102

[37]Wang Y Q, Zhang J J, Zhu G H, Peng X X. Differential expression of proteins in rice leaves cultivated with different forms of nitrogen nutrients. J Plant Physiol Mol Biol, 2006, 32: 403-410

[38]Zhao S-P(赵首萍), Shi W-M(施卫明). Expression patterns of osAMT(1.1-1.3), osAMT3.1 and osAMT 4.1 in rice (Oryza sativa L.). Soil (土壤), 2007, 39(3): 460-464 (in Chinese with English abstract)
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