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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2118-2127.doi: 10.3724/SP.J.1006.2014.02118


Genetic Diversity of Gulin-niupicha (Camellia sinensis [L.] O. Kuntze) Germplasm Resources

LIU Shao-Jie1,2,CHI Lin2,XIE Wen-Gang1,HAN Nan1,CHEN Ying-Hui2,TANG Qian1,*   

  1. 1College of Horticulture, Sichuan Agricultural University, Ya’an 625014, China?; 2Department of Agriculture, Forestry and Water Conservancy, Chongqing Economic and Trade Vocational School, Chongqing 402100, China
  • Received:2014-04-17 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-01
  • Contact: 唐茜, E-mail: tangqi2008@126.com


Gulin-niupicha (Camellia sinensis)is an specific tea landrace population grown in Jiaozigou, Gulin County, Sichuan Province, China. To protect and make use of the tea germplasm resources effectively, we conducted a study investigating the morphologic characteristics, biochemical components and genetic diversity of Gulin-niupicha germplasm resources in which a total of 76 individuals from seven populations were used to identify the phenotypic and genetic traits by data collection, samples analysis and inter-simple sequence repeats (ISSR) analysis. The results showed that: there were various biological characteristics among the seven populations. Importantly, a majority of Gulin-niupicha were shrubs, with semi-tree rescent form. Their mature leaves were medium in size and elliptic or long elliptic in shape, with green or dark green and the color in tender shoot varied including white, light green, green, dark green and violet-blue. Moreover, in that light of determination of biochemical components, free amino acid content was higher in two populations (AA>4.5%), and tea polyphenols (TP>27%, spring; TP>31%, summer) and catechins (>150 mg g-1, spring; > 190 mg g-1, summer) content were both higher in two populations, while the among of TP/AA ratio of these populations was 3.84~16.95, which indicated that the Gulin-niupicha has a high value in tea plant breeding and deep processing. Consistently, 135 DNA fragments among the 76 tea accessions were amplified using 15 ISSR primers, 122 bands of which were polymorphic (PPB=90.4%). Nei’s gene diversity (H) and Shannon’s information index (I) were 0.254 and 0.392, respectively, indicating that the diversity of the tested tea germplasm is relatively low.

Key words: Gulin-niupicha (Camellia sinensis L.), Germplasm resources, Genetic diversity, ISSR

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