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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 533-538.doi: 10.3724/SP.J.1006.2010.00533

• RESEARCH ACTIVITIES • Previous Articles    

Cytoplasmic Alkalization Mediates Exogenous Nitric Oxide-Induced Stomatal Closure in Vicia faba

ZHAO Shi-Ling,SUN Li-Rong,ZHANG Huan,MA Li-Ya,LU Bao-Shi,HAO Fu-Shun*   

  1. Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng 475004, China
  • Received:2009-07-17 Revised:2009-12-08 Online:2010-03-12 Published:2010-01-22
  • Contact: HAO Fu-Shun, E-mail: haofsh@henu.edu.cn


The effects of exogenous NO on changes in cytosolic pH of guard cells from epidermal fragments and of the pH on NO-induced stomatal closure in Vicia faba were investigated by using a pH specific fluorescence probe SNARF-1-AM and a confocal laser scanning microscopy. The results showed that treatments with 100 μmol L–1 sodium nitroprusside (SNP), a widely used NO donor, led to a significant increase in cytosolic pH (from 6.91 to 7.19, i.e. 0.28 pH unit) and stomatal closure in comparison with the control. Furthermore, SNP-induced cytosolic alkalization and stomatal closure were significantly inhibited by NO scavenger c-PTIO or weak acid butyrate, and promoted by the weak base benzylamine. Whereas two structural analogs of SNP such as Fe(II)CN and Fe(III)CN, which can not release NO, failed to induce guard cell cytoplasmic alkalization and stomatal closure. These findings suggest that cytoplasmic alkalization of guard cells mediates NO-induced stomatal closure. In addition, pH in guard cell vacuoles and cell walls was measured during the cytosolic alkalization. However, no significant changes of pH in the two regions were found, implying that the cytoplasmic protons of guard cells may not go into vacuoles or cell walls during the NO-promoted alkalization in Vicia faba.

Key words: Nitric oxide, pH, Vicia faba, Stomatal closure

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