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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 405-414.doi: 10.3724/SP.J.1006.2011.00405

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Marker Genotypes Associated with Elite Combining Ability for Quality Traits in Parents of Hybrid Japonica Rice (Oryza sativa L.)

HUANG Dian-Cheng1,2, LIANG Kui1,**,SUN Cheng1,JIANG Jian-Hua,SHI Ming-Liang3,DAI Jian4,XIE Hui5,ZHAO Kai-Ming1,NGUYEN Phuong-Tung6,MA Wen-Xia1,HONG De-Lin1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 3 Institute of Agricultural Sciences of the Area Along Yangtse of Jiangsu, Rugao 226541, China; 4 Institute of Food Safety Research and Inspection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 5 Tianjin Subcentre of China National Hybrid Rice Research and Development Center, Tianjin 300457, China; 6Agricultural Science Institute of Northern Central Vietnam, Nghe An-Vinh, Vietnam
  • Received:2010-08-30 Revised:2010-12-06 Online:2011-03-12 Published:2011-01-17

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Abstract: The area planting japonica hybrid rice is only 3% of the total area (8.28´106 ha) of japonica rice. One of the reasons why japonica hybrid rice popularized slowly is that grain quality of hybrid was inferior to that of conventional cultivars. In order to improve grain quality combining ability of parental lines more efficiently, SSR marker genotypes of elite combining ability for 10 quality traits, namely, brown rice rate (BRR), milled rice rate (MRR), head rice rate (HRR), grain length (GL), grain width (GW), percentage of chalky grain (PCG), chalkiness degree (CD), gelatinization temperature (GT), gel consistency (GC) and amylose contents (AC), were identified in this study, by analyzing the data of combining ability and SSR markers in six CMS lines and twelve restorer lines, which were genotyped using 115 pairs of SSR primers. A total of 30 SSR marker genotypes were significantly associated with combining ability of the 10 quality traits. Twenty-five of them were marker genotypes for unfavorable combining ability of quality traits. The remaining five marker genotypes were associated with elite combining ability for quality traits of parents. RM263-175/180 and RM444-230/240 were marker genotypes of elite combining ability for HRR, which increased 3.2% and 2.5% of HRR in F1, respectively. RM3-120/150 shortened GL value in F1 by 2.4%. RM444-180/240 increased GW trait value of F1 by 2.1%. RM428-273/294 decreased AC trait value of hybrid rice harvested from F1 plants by 7.0%. Among the SSR marker genotypes associated with combining ability of the 10 quality traits, eight marker genotypes also affected combining ability of yield components simultaneously. RM3-120/150 increased simultaneously total spikelets per plant (TSP) and filled spikelets per plant (FSP) in F1 by 15.9% and 10.9%, respectively. RM1211-150/160 decreased trait values of BRR and MRR in F1 by 0.9% and 1.1%, respectively, and simultaneously increased trait values of TSP and FSP in F1 by 21.8% and 20.4%. RM23-150/160 increased trait values of PCG and CD in F1 by 44.1% and 45.7%, and simultaneously increased daily yield per plant and TSP in F1 by 11.2% and 11.6%, respectively. These results could be used for directing improvement of combining ability of parents for quality traits and yield traits through the marker-assisted selection, and for determining parents for making combinations of hybrid japonica rice in the future.

Key words: Japonica rice, Combining ability, SSR, Grain quality, Marker genotypes

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