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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 223-230.doi: 10.3724/SP.J.1006.2012.00223

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transferability of Mungbean Genomic-SSR Markers in Other Vigna Species

ZHONG Min, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, WANG Xiao-Bao   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China?
  • Received:2011-07-07 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-01
  • Contact: 程须珍, E-mail: chengxz@caas.net.cn, Tel: 010-62180535

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Abstract: Cowpea (Vigna unguiculata L. Walp.), mungbean (V. radiata L. Wilczek), rice bean [V. umbellate (Thunb.) Ohwi & Ohashi] and adzuki bean (V. angularis Willd. Ohwi and Ohashi) are major food legumes in China. At present, SSR markers for genetic analysis of these legumes are much limited. Transferability analysis of primers has the vital significance to reduce their development cost and improve their development efficiency. In this study, 1 205 SSRs primers were tested for their transferability and polymorphism by PCR amplification with the genomic DNA of four Vigna species, cowpea, adzuki bean, mungbean and rice bean. The results indicated that the transferability rate of mungbean genomic-SSR in cowpea, adzuki bean and rice bean was 50.0%, 73.3%, and 81.6%, and the ratio of polymorphism SSR primers in these crops was 4.1%, 1.7%, and 1.5%, whereas 32.0% in mungbean. A total of 469 mungbean genomic-SSR primers were detected to be highly transferable among different species of Vigna. The transferability of mungbean genomic-SSR was higher in adzuki bean and rice bean than in cowpea. These transferable markers are useful for further genetic and breeding studies in these species.

Key words: Genus Vigna, SSR, Transferability

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