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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 840-847.doi: 10.3724/SP.J.1006.2012.00840

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Evaluation of Genetic Relationship in Ramie Based on RSAP, SRAP, and SSR

ZOU Zi-Zheng1,2,CHEN Jian-Hua1,*,LUAN Ming-Bao1,*,GUO Jin-Xia2,WANG Chao3,WANG Xiao-Fei1,XU Ying1,SUN Zhi-Min1   

  1. 1 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key Laboratory of Stem fiber Biomass and Engineering Microbilogy, Ministry of Agriculture, Changsha 420105, China; 2 Yiyang Medical School, Yiyang 413000, China; 3 School of Biological Science and Technology, Central South University, Changsha 410205, China
  • Received:2011-10-13 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 陈建华, E-mail: cjhbt@sina.com, Tel: 13908494958; 栾明宝, E-mail: luanmingbao2002@126.com, Tel:0731-88998518 E-mail:zouzizheng@hotmail.com

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Abstract: Ramie [Boehmeria nivea (L.) Gaud.] researches in molecular biology lags behind, there is a necessary to develop molecular markers in the genetic relationship analysis of ramie. In this paper, three molecular markers-RSAP, SRAP and SSR were used to cluster 16 Ramie germplasm. The result showed that each pair of primers could provide polymorphic sites and the site number was SRAP>RSAP>>SSR. Both results clustered based on SRAP and RSAP showed high consistency with that clustering based on combination of SRAP, RSAP and SSR, and the highly significant correlation coefficients were given. However, results of clustering based on SSR marker were quite different from the results based on combined markers. The rank order of similarities between the clustering results based on the molecular marker and phenotype was SRAP+RSAP+SSR>SRAP>RSAP>>SSR. For the study of genetic relationship among ramie germplasm, the efficiency of SRAP marker was slightly higher than that of RSAP marker, but that of SSR was low. Based on RSAP marker could efficiently display the polymorphism and genetic relationship among ramie varieties. Using the combined markers of RSAP&SRAP&SSR could be to reveal the genetic relationship of germplasm.

Key words: Ramie, RSAP, SRAP, SSR, Relationship

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