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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1127-1134.doi: 10.3724/SP.J.1006.2012.01127

• RESEARCH NOTES • Previous Articles     Next Articles

Expression of Potassium Metabolism-Related Gene in Tobacco

XIA Kai1,2,XU Shuang-Hong1,WANG Xiang1,DAI Lin-Jian2,LI Peng-Fei3,LUO Jian-Xing2,QI Shao-Wu2,YANG Qiong2,ZHOU Qing-Ming2,*   

  1. 1 Hunan Tobacco Industrial Co., Ltd, Changsha 410014, China; 2 Hunan Agricultural University, Changsha 410128, China; 2 Wenshan Company of Yunnan Provincial Tobacco Company, Wenshan 663000, China
  • Received:2011-09-21 Revised:2012-01-15 Online:2012-06-12 Published:2012-04-06
  • Contact: 周清明, E-mail: zqm8051@hunau.net, Tel: 0731-84618051 E-mail:xiak0502@hngytobacco.com

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Abstract: The quality of flue-cured tobacco in production is currently limited by low potassium level in leaf. A simple and effective way to alleviate the supply of potassium in soil is screening the potassium-enriched flue-cured tobacco types. In the experiment, the tobacco seedlings were cultured in nutrient solution, and then replanted in sand soil. To investigate the potassium metabolism related gene’s expression and kinetic parameters of potassium uptake and utilization with six different lines including five potassium-enriched genotypes of K2, K3, K5, K7, and K9 and a conventional genotype of K326. The results showed that the tobacco lines K2, K7, and K9 were typical potassium-enriched genotypes, TORK1 and NtTPK1 out of 12 genes performed high expression. The ability of potassium absorption of K3, K5, K9, K7, and K2 was significantly stronger than that of K326. The potassium absorption abilities of potassium -enriched types of K9, K2, K7, K3, and K5 were stronger but their use efficiencies were lower than these of K326 in high potassium environment, and higher in low potassium environment, especially for the genotypes K2, K7, and K9.

Key words: Potassium metabolism, Gene, Expression, Nicotiana tabacum

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