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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 43-49.doi: 10.3724/SP.J.1006.2013.00043

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity of Waxy Gene in Chinese Glutinous Maize

YAO Jian-Qiang1,2,BAO Jian-Dong2,3,ZHU Jin-Qing1,GUI Yi-Jie2,SHEN Qiu-Fang2,HU Wei-Min2,FAN Long-Jiang2,*   

  1. 樊龙江, E-mail: fanlj@zju.edu.cn, Tel: 0571-88982730
  • Received:2012-05-04 Revised:2012-08-15 Online:2013-01-12 Published:2012-10-08

Abstract:

Waxy (Wx) gene is the key gene for glutinous trait in many waxy crops including waxy maize. Wx diversity investigation in maize population has important significance for quality breeding and germplasm utility of Chinese waxy maize. In this study, 325 waxy maize germplasm accessions (309 from China, 11 from Thailand and five from South Korea) were used to investigate their wx genotypes and determine their apparent amylose content (AAC). Our results demonstrated that genetic diversity of wx gene in Chinese waxy maize was low, and two mutation types, wx-D7 andwx-D10, account for 96.9% of the 325 accessions. Among these accessions, those from North of China and South Korea were the type of wx-D7 while others from South of China and Thailand were the type of wx-D10; most of landraces were the type of wx-D10 (78.9%) while most commercial hybrids and inbred lines were the type of wx-D7 (88.7% and 86.6%, respectively). AAC estimations indicated that mean values of AAC in commercial hybrids and inbred lines in China were lower than 2.2% while that in landraces was relatively high. Comparing with wx-D7, wx-D10 waxy corn had higher AAC with larger changing range. Geographical distribution and origins of two main wx genotypes and other wx genotypes were discussed also.

Key words: Glutinous maize, Waxy, Genetic diversity, Investigation, Apparent amylose content (AAC)

[1]Fedoroff N, Wessler S, Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell, 1983, 35: 235–242



[2]Hirano H Y, Sano Y. Molecular characterization of the waxy locus of rice (Oryza sativa). Plant Cell Physiol, 1991, 32: 989–997



[3]Wang Z Y, Zheng F Q, Shen G Z, Gao J P, Snustad D P, Li M G, Zhang J L, Hong M M. The amylose content in rice endosperm is related to the posttranscriptional regulation of the waxy gene. Plant J, 1995, 7: 613–622



[4]Hirano H Y, Eiguchi M, Sano Y. A single base change altered the regulation of the waxy gene at the posttranscriptional level during the domestication of rice. Mol Biol Evol, 1998, 15: 978–987



[5]Isshiki M, Morino K, Nakajima M, Okagaki R J, Wessler S R, Izawa T, Shimamoto K. A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5’ splice site of the first intron. Plant J, 1998, 15: 133–138



[6]Olsen K M, Purugganan M D. Molecular evidence on the origin and evolution of glutinous rice. Genetics, 2002, 162: 941–950



[7]McIntyre C L, Drenth J, Gonzalez N, Henzell R G, Jordan D R. Molecular characterization of the waxy locus in sorghum. Genome, 2008, 51: 524–533



[8]Domon E, Fuijita M, Ishikawa N. The insertion/deletion polymorphisms in the waxy gene of barley genetic resources from east Asia. Theor Appl Genet, 2002, 104: 132–138



[9]Patron N J, Smith A M, Fahy B F, Hylton C M, Naldrett M J, Rossnagel B G, Denyer K. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase i with a deletion in the 5'-non-coding region. Plant Physiol, 2002, 130: 190–198



[10]Hunt H V, Denyer K, Packman L C, Jones M K, Howe C J. Molecular basis of the waxy endosperm starch phenotype in broomcorn millet (Panicum miliaceum L.). Mol Biol Evol, 2010, 27: 1478–1494



[11]Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T. Production of waxy (amylose-free) wheats. Mol Gen Genet, 1995, 248: 253-259



[12]Wessler S R, Varagona M J. Molecular basis of mutations at the waxy locus of maize: correlation with the fine structure genetic map. Proc Natl Acad Sci USA, 1985, 82: 4177–4181



[13]Wessler S, Tarpley A, Purugganan M, Spell M, Okagaki R. Filler DNA is associated with spontaneous deletions in maize. Proc Natl Acad Sci USA, 1990, 87: 8731–8735



[14]Marillonnet S, Wessler S R. Retrotransposon insertion into the maize waxy gene results in tissue-specific RNA processing. Plant Cell, 1997, 9: 967–978



[15]Liu J, Rong T Z, Li W C. Mutation loci and intragenic selection marker of the granule-bound starch synthase gene in waxy maize. Mol Breed, 2007, 20: 93–102



[16]Klosgen R B, Gierl A, Schwarzsommer Z, Saedler H. Molecular analysis of the waxy locus of Zea mays. Mol Biol Evol, 1986, 203: 237–244



[17]Fan L J, Quan L Y, Leng X D, Guo X Y, Hu W M, Ruan S L, Ma H S, Zeng M Q. Molecular evidence for post-domestication selection in the waxy gene of Chinese waxy maize. Mol Breed, 2008, 22: 329–338



[18]Fan L J, Bao J D, Wang Y, Yao J Q, Gui Y J, Hu W M, Zhu J Q, Zeng M Q, Li Y, Xu Y B. Post-domestication selection in the maize starch pathway. PLoS One, 2009, 4: e7612



[19]Tian M-L(田孟良), Huang Y-B(黄玉碧), Tan G-X(谭功燮), Liu Y-J(刘永建), Rong T-Z(荣廷昭). Sequence polymorphism of waxy genes in landraces of waxy maize from Southwest China. Acta Agron Sin (作物学报), 2008, 34(5): 729–736 (in Chinese with English abstract)



[20]Okagaki R J, Neuffer M G, Wessler S R. A deletion common to two independently derived waxy mutations of maize. Genetics, 1991, 128: 425–431



[21]Huang Y-B(黄玉碧), Rong T-Z(荣廷昭). Genetic diversity and origin of Chinese waxy maize. Crop (作物杂志), 1998, (suppl): 77–80 (in Chinese)



[22]Zeng M-Q(曾孟潜), Yang T-X(杨太兴), Wang P(王璞). The relative analyses on maize cultivar Menghai Four-Row Wax. Acta Genet Sin (遗传学报), 1981, 8(1): 91-96 (in Chinese with English abstract)



[23]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4325



[24]Shin J H, Kwon S J, Lee J K, Min H K, Kim N S. Genetic diversity of maize kernel starch-synthesis genes with SNAPs. Genome, 2006, 49: 1287–1296



[25]Bao J D, Yao J Q, Zhu J Q, Hu W M, Cai D G, Li Y, Shu QY, Fan L J. Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene. Mol Breed, 2012 (Published online: DOI 10.1007/s11032-012-9754-3)

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